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Microculture of single protoplasts ofBrassica napus

 

作者: G. Spangenberg,   H.‐U. Koop,   R. Lichter,   H. G. Schweiger,  

 

期刊: Physiologia Plantarum  （WILEY Available online 1986）
卷期: Volume 66, issue 1  

页码: 1-8

 

ISSN:0031-9317

 

年代: 1986

 

DOI:10.1111/j.1399-3054.1986.tb01223.x

 

出版商: Blackwell Publishing Ltd

 

关键词: Callus formation;hypocotyl protoplasts;individual culture;rapeseed

 

数据来源: WILEY

 

摘要:

Protoplasts ofBrassica napusL. were cultured individually in a microdroplet system using a synthetic medium with survival rates of more than 70% and division frequencies of up to 65%. Microcallus formation occurred at frequencies of up to 50%. Factors affecting the survival and division of individually cultured protoplasts, such as composition and volume of culture medium, pH, buffering system, osmolarity and genotype, were analyzed.

 

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