Selective Determination of Cytochromecby Capillary Zone Electrophoresis with Amperometric Detection at a L-Cysteine-Modified Gold Microdisk Array Electrode
作者:
Wenrui Jin,
Qianfeng Weng,
Jianren Wu,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 4
页码: 753-769
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708006422
出版商: Taylor & Francis Group
关键词: Capillary zone electrophoresis;Electrochemical detection;Cytochromec;Modified electrode
数据来源: Taylor
摘要:
Capillary zone electrophoresis was employed for the analysis of cytochromecusing an end-column amperometric detector with a L-cysteine-modified gold microdisk array electrode. The optimum conditions of separation and detection are 0.04 mol/L KH2PO4- 0.06 mol/L Na2HPO4for the buffer solution, 8 kV for the separation voltage, 4 kV and 10 s for the injection voltage and the injection time, and −0.12 V vs. SCE for the detection potential. When the fused-silica capillary modified by ethylene glycol was used, the adsorption of cytochromecon the surface of the capillary wall could be overcome. The limit of detection is 3 × 10−5mol/L or 62 fmol (S/N = 2). The method was applied to determining cytochromecin pig heart.
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