A Fiber Optic Biosensor for Uric Acid Based on Immobilized Enzymes
作者:
Zhilong Gong,
Zhujun Zhang,
期刊:
Analytical Letters
(Taylor Available online 1996)
卷期:
Volume 29,
issue 5
页码: 695-709
ISSN:0003-2719
年代: 1996
DOI:10.1080/00032719608001777
出版商: Taylor & Francis Group
关键词: Uricase;Thiamine;Fluorescence;Horseradish peroxidase
数据来源: Taylor
摘要:
A simplified enzyme based fiber optic uric acid biosensor is reported. It is constructed by coimmobilizing uricase and horseradish peroxidase [HRP] to bovine albumin via glutaraldehyde. A new fluorimetric substrate, thiamine, is used to indicate the sensing process. Under optimized conditions, the linear range of uric acid concentration is 0.5 to 5.0 ug/mL with a correlation coefficient of 0.997, and the detection limit is 0.15 ug/mL with excellent reversibility and stability. The sensor can be used at least two months at room temperature. It is possibly to determine uric acid directly in serum and urine samples with satisfactory results.
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