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Feulgen Banding in Unfixed Metaphase Chromosomes of Some Plant Species

 

作者:

 

期刊: Caryologia  (Taylor Available online 1981)
卷期: Volume 34, issue 1  

页码: 83-88

 

ISSN:0008-7114

 

年代: 1981

 

DOI:10.1080/00087114.1981.10796875

 

出版商: Taylor & Francis

 

数据来源: Taylor

 

摘要:

Chromosomes of unfixed root tips hydrolysed for 7 minutes and stained by the Feulgen method show band patterns paralleling in reverse style those shown by Q-staining inScilla sibirica, Ornithogalum montanum, Vicia fabaandBrimeura amethistina: Q-bands of reduced fluorescence appear deeply stained inScilla sibirica, while brightly fluorescing Q-bands appear as colourless gaps inOrnithogalum montanum, Vicia faba and Brimeura amethistina.Whether such phenomenon is caused by condensation or loose packing of DNA at band level or simply to differential reactivity of DNA to the Feulgen stain is briefly discussed.

 

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