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Verotoxin-Binding in Human Renal Sections

 

作者: C.A. Lingwood,  

 

期刊: Nephron  (Karger Available online 1994)
卷期: Volume 66, issue 1  

页码: 21-28

 

ISSN:1660-8151

 

年代: 1994

 

DOI:10.1159/000187761

 

出版商: S. Karger AG

 

关键词: Globotriaosyl ceramide;Renal glomerulus;Hemolytic uremic syndrome;Fluorescence microscopy

 

数据来源: Karger

 

摘要:

Gastrointestinal infection with verotoxin-producing Escherichia coli (VTEC) has been strongly implicated in the etiology of the hemolytic uremic syndrome (HUS), the leading cause of pediatric acute renal failure. The binding of fluorescein-conjugated VT1 overlaid on to frozen human renal sections has been examined. Sections from biopsies of infants aged < 2 years were compared with those from adult autopsies. VT primarily stained distal convoluted tubules, particularly those adjacent to glomeruli, and collecting ducts. VT-binding was detected within the infant glomerulus but not the adult. Binding of the toxin was removed when the section was pretreated with α-galactosidase, confirming the receptor-binding specificity for globotriaosyl ceramide (galα1-4galβ1-4 glucosyl-ceramide), the glycolipid receptor for VT. These studies may suggest that differential localization of this glycolipid in the pediatric renal glomerulus is a risk factor for the development of HUS following infection with VT

 

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