THE EUROTRANSPLANT HLA‐DRB OLIGONUCLEOTIDE TYPING SET
作者:
M.J. Giphart,
W. Verduijn,
期刊:
International Journal of Immunogenetics
(WILEY Available online 1991)
卷期:
Volume 18,
issue 1‐2
页码: 57-68
ISSN:1744-3121
年代: 1991
DOI:10.1111/j.1744-313X.1991.tb00006.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
SUMMARYSeveral sequence‐specific oligonucleotides (SSO) were designed based upon available sequences of HLA‐DRB 1 genes which are associated with HLA‐DR serotypes. Targets for these SSO were polymerase chain reaction (PCR) products using primers specific for the second exon of DRB genes. In view of practicality, a hybridization system was developed which was independent of temperature or composition of the hybridization buffers, hence uniformly applicable to all SSO, yet allowing discrimination based upon one nucleotide mismatch only. The SSO typing results were compared with HLA serological typing and a high correlation was observed
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