SummaryThe limitations and pitfalls of methods of amylase determination in biological fluids are discussed. Amyloclastic technics arc going to be more and more widely performed. They are easier, more sensitive than saccharogenic technics.The various methods of amylase determination are to be compared and standardized in order to avoid discrepancies of the results. Clinicians should be aware of the technical pitfalls which may introduce fallacious interpretations.As far as the pancreatic iso-amylase is concerned, further research is necessary before clinical applications.