Effect of dipyridamole on vascular responses of porcine ciliary arteries
作者:
MeyerPeter,
FlammerJosef,
LücherThomas F.,
期刊:
Current Eye Research
(Taylor Available online 1996)
卷期:
Volume 15,
issue 4
页码: 387-393
ISSN:0271-3683
年代: 1996
DOI:10.3109/02713689608995829
出版商: Taylor&Francis
关键词: ciliary arteries;dipyridamole;ditric oxide;prosta-cyclin;endothelin-1;pig;vasodilation
数据来源: Taylor
摘要:
This study investigated the effects of dipyridamole in isolated porcine ciliary arteries (diameter 200–250μm).Isolated porcine ciliary arteries were suspended in myograph chambers filled with modified Krebs-Ringer solution (37°C; 95% O2/5% CO2) for isometric tension recording.Dipyridamole induced concentration-dependent relaxations of porcine ciliary arteries with endothelium precontracted with thromboxane analogue U-46619 (10-6M), KC1 (50 mM), or endothelin-1 (10-8M). Removal of the endothelium of the ciliary vessels and preincubation of the arteries with L-NAME (10-SM), or indomethacin (10-5M), or the combination of the two drugs significantly reduced the relaxation to dipyridamole (p = 0.002–0.03). Similar vascular responses could be observed in a time-dependant analysis of the effect of a single concentration dipyridamole (10-4M). The stimulator of CAMP forskolin also caused relaxations. Endothelin-1 (10-12-10-7M) and U-46619 (1010-10−6M) induced potent contractions of porcine ciliary arteries. Preincubation with dipyridamole (10−5M) reduced contractions to endothelin-1 as compared to control (p<0.004), while contractions to U-46619 were only slightly affected under these conditions (n.s.).These findings demonstrate that dipyridamole is a vasodilator in porcine ciliary arteries. Endothelial nitric oxide and prosta-cyclin contribute importantly to the effects of dipyridamole. Further studies are required to show whether these properties of dipyridamole may also occurin vivoand offer clinical use in patients with ocular vasospasms and other ophthalmic vascular dysfunctions.
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