Engineering Antibiotic Producers to Overcome the Limitations of Classical Strain Improvement Programs
作者:
LalRup,
KhannaRichie,
KaurHardeep,
KhannaMonisha,
DhingraNidhi,
LalSukanya,
HeinzKarl,
EichenlaubRudolf,
GhoshP. K.,
期刊:
Critical Reviews in Microbiology
(Taylor Available online 1996)
卷期:
Volume 22,
issue 4
页码: 201-255
ISSN:1040-841X
年代: 1996
DOI:10.3109/10408419609105481
出版商: Taylor&Francis
关键词: Streptomyces lincolnensis;isopenicillin-N-synthase;pcbC;anthracycline;Streptomyces violaceorüber;DAOCS/DAOCH;shikimate;rifamycins;Amycolatopsis mediterranei
数据来源: Taylor
摘要:
AbstractImprovement of the antibiotic yield of industrial strains is invariably the main target of industry-oriented research. The approaches used in the past were rational selection, extensive mutagenesis, and biochemical screening. These approaches have their limitations, which are likely to be overcome by the judicious application of recombinant DNA techniques. Efficient cloning vectors and transformation systems have now become available even for antibiotic producers that were previously difficult to manipulate genetically. The genes responsible for antibiotic biosynthesis can now be easily isolated and manipulated. In the first half of this review article, the limitations of classical strain improvement programs and the development of recombinant DNA techniques for cloning and analyzing genes responsible for antibiotic biosynthesis are discussed. The second half of this article addresses some of the major achievements, including the development of genetically engineered microbes, especially with reference toβ-lactams, anthracyclines, and rifamycins.
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