Permanent Stained Preparations of Synovial Fluid for Detection of Calcium Compounds Using Alizarin Red S
作者:
LazcanoOscar,
BilbaoJorge,
BeissnerRobert S.,
VandiverMartha,
LiChin Yang,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 1
页码: 14-20
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209109999
出版商: Taylor&Francis
关键词: synovial fluid;calcium compounds;alizarin red S;calcium pyrophosphate dihydrate;calcium oxalate
数据来源: Taylor
摘要:
Permanent preparations of air dried synovial fluids were prepared by staining calcium compounds with alizarin red S stain; each slide was coverslipped with Permount. Variables studied were: (a) concentration of the solution of alizarin red S, (b) pH of staining solution, (c) time of incubation in staining solution and aqueous and ethanolic content of staining solution. The staining effect of each solution was tested on calcium pyrophosphate dihydrate, calcium oxalate, apatite and monosodium urate (MSU). Of all the solutions, best results were obtained with 0.25% alizarin red S in 50% ethanol at pH 7.0 for 30 min. With this solution, the calcium-containing compounds were well stained. MSU did not stain and still preserved negative birefringence on polarizaton. Fixation of smears with ethanol served a double purpose: It fixed the slides without dissolving or removing MSU or the calcium compounds, yet it did dissolve five corticosteroids commonly used for intra-articular injection which may interfere with interpretation of compensated polarized light microscopy of synovial fluids.
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