摘要:

AbstractA human T lymphoid cell line was established by cell hybridization technique from peripheral blood leucocytes of a patient with Sezary syndrome. The cells beared the surface antigens of human T lymphocyte specificity as demonstrated by immune cytolysis tests, but did not form E rosettes with sheep red blood cells. Isozyme patterns of enzymes in this line such as lactate dehydrogenase, glucose 6‐phosphate dehydrogenase and esterase were of human type. The line had 79 chromosomes in modal number. This case supports the proposal that the production of tetraploids is favourable for establishment of cell line

ISSN:0021-9541    

DOI:10.1002/jcp.1040940112

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractThe unknown biochemical role of arginase in epidermal metabolism was probed by examining the association of elevated arginase activty with epidermal hyperplasia and hyperkeratinization. Epidermal hyperplasia as induced experimentally by topical application of 1‐decanol to the right side of male hairless mice while the contralateral side served as control. Arginase activity, incorporation of3H‐thymidine into DNA, DNA and protein content were measured in the separated control and experimental epidermis six hours and on days 1 through 5 and 7 after 1‐decanol application. After six hours, the epidermis appears damaged histologically, and DNA synthesis is inhibited. By day 1, incoporation of3H‐thymidine into DNA is elevated and a new hyperplastic epidermis has formed beneath the original epidermis. Epidermal arginase is elevated two through seven days after 1‐decanol application and always is associated with continuing epidermal hyperplasia. The stimulation of DNA synthesis, which parallels the induction of epidermal hyperplasia by 1‐decanol, precedes the induction of epidermal arginase activity. An attempt to relate these results with polyamine synthesis and other metabolic eve

ISSN:0021-9541    

DOI:10.1002/jcp.1040940113

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

摘要:

AbstractSome properties of the enzyme phosphoglucose isomerase (PGI) from the foetal lung fibroblast strain MRC‐5 have been investigated throughout the in vitro lifespan of this cell strain.No significant age‐related alterations in specific activity or thermostability of PGI could be detected. Titration of enzymatic activity with antibody directed against purified PGI showed no detectable differences in PGI from extracts of early passage cells compared with enzyme from senescent cells.The effect of p‐fluorophenylalanine incorporation on PGI was examined in early passage fibroblasts. Thermostability studies showed increased heat lability of PGI from analogue treated cells when compared with enzyme from control cells at the same passage. However, no inactive PGI protein could be detected by antiserum titration in extracts from analogue‐treated cells.The results indicate that no significant amount of altered or inactive PGI is produced in ageing fibr

ISSN:0021-9541    

DOI:10.1002/jcp.1040940114

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY

ISSN:0021-9541    

DOI:10.1002/jcp.1040940101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1978

数据来源: WILEY