摘要:

AbstractWe have designed an in vitro assay to assess the influence of tumor subpopulation interactions on drug response. The assay is based upon inhibition of growth of 1 mm3‐pieces of tumor embedded in a collagen gel matrix. Tumor growth is quantitated by planimetry of each colony's image, formed with a split image tracing device attached to an inverted microscope. That expansion of the colonies in collagen gel represents growth through cell replication was demonstrated by releasing and counting cell nuclei. Outgrowths from pieces of tumors produced by a series of mouse mammary tumor subpopulation lines expanded in collagen gel at a rate characteristic of each cell line: the growth rate of tumor pieces was similar to that of the corresponding tumor line embedded as a cell bolus of cultured cells, indicating that growth of pieces of tumor is due to the tumor cells rather than to stromal components. When two cell lines were grown together in collagen cultures, interactions affecting growth rate were observed. Both tumor pieces and cell boluses from cultured cells of the relatively homogeneous cell lines displayed similar, characteristic sensitivities to adriamycin (ADR) in the collagen gel assay. Advantages of the collagen assay over cloning assays are (1) preservation of potential cellular interactions which may be important in assessing tumor drug sensitivity; (2) maximization of growth of all cell populations within the tumor, as compared to growth in agar; and (3) reflection of the zonal distribution of different subpopulations within tumors; and (4) simulation of the three‐dimensional growth architecture found in v

ISSN:0021-9541    

DOI:10.1002/jcp.1041210413

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0021-9541    

DOI:10.1002/jcp.1041210414

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0021-9541    

DOI:10.1002/jcp.1041210415

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0021-9541    

DOI:10.1002/jcp.1041210416

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractIn cells transformed by avian sarcoma viruses or stimulated by growth factors, certain polypeptides become phosphorylated at tyrosine residues. It is not known if these cellular polypeptides are phosphorylated directly by the tyrosine‐kinase activities which are associated with the viral transforming proteins and with growth factor receptors. It is also not clear if phosphorylation of these polypeptides is required for viral transformation or the response to growth factors. We describe here some observations which bear on these questions and discuss possible future approache

ISSN:0021-9541    

DOI:10.1002/jcp.1041210417

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0021-9541    

DOI:10.1002/jcp.1041210418

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractChromosome abnormalities in five solid tumors which occur predominantly in children and young adults are described. Four of the tumors are associated with rearrangements involving particular chromosome segments: deletion 13q14 in retinoblastoma, deletion 11p13 in Wilms' tumor, deletions/rearrangements of 1p (distal to band 1p31) in neuroblastoma, and translocation 22/autosome in Ewing sarcoma. Though aneuploidy for a number of chromosomes may occur in the fifth tumor, rhabdomyosarcoma, it has yet to be associated with a consistent marker arrangement. A general classification of chromosome abnormalities in cancer is presented. Possible roles for the gene changes produced by each class of chromosome abnormality in the development of cancer are discussed.

ISSN:0021-9541    

DOI:10.1002/jcp.1041210419

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractChromosomal translocations are found to be a characteristic feature of Burkitt lymphomas. Similar translocations are found in mouse plasmacytomas and both diseases involve interchanges between one of the immunoglobulin loci and DNA in the vicinity of themycgene. The structure of themycgene has been elucidated from studies on translocated versions of the gene. Activation of themycgene may play a role in transformation by promoting growth of the cells bearing the rearranged chromosomes.

ISSN:0021-9541    

DOI:10.1002/jcp.1041210420

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0021-9541    

DOI:10.1002/jcp.1041210421

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0021-9541    

DOI:10.1002/jcp.1041210422

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY