摘要:

Cell-free supernatant of cultures fromMycoplasma arthritidis(MAS) functions as an extremely potent T-cell mitogen for human and murine lymphocytes. The T-cell response is dependent on the presence of accessory cells, presenting the intact E, molecule on the cell surface. Until now, pure MAS protein has not been available. We developed a new multi-step method for MAS purification. The main steps in this protocol are ammonium sulfate precipitation, anion exchange and hydroxyapatite chromatography followed by gel filtration. With this efficient protocol we obtained fractions of extremely potent mitogenic properties, the purification rate was about 5×105. Although this protease-sensitive mitogenic activity was highly enriched. we failed to detect the protein by sensitive staining methods of SDS-PAGE. In previous studies, we showed that MAS induces the synthesis of interferon gamma in human and murine lymphocyte cultures. Here we demonstrate that MAS induces interleukin-6 (IL-6) in murine bone-marrow derived macrophage cultures. Since IL-6 is also induced by endotoxin, we used C3H/HeJ mice, which are known to be LPS-nonresponders, in all our studies.

ISSN:0891-6934    

DOI:10.3109/08916939009087591

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

ISSN:0891-6934    

DOI:10.3109/08916939009087592

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor