摘要:

We investigated interleukin 6 (IL-6) mRNA expression in peripheral blood T-cells obtained from 36 patients with IgA nephropathy (IgAN), 36 patients with other glomerulonephritides and 24 healthy age-matched controls. The majority of patients with IgAN had increased IL-6 mRNA expression by their T cells; no IL-6 mRNA was detected in T cells obtained from patients with other glomerulonephritides or normal controls. A positive correlation was noted between the IL-6 mRNA level and quantity of protein excretion in the urine, histopathological findings, and renal function. However, there was no significant correlation between IL-6 mRNA expression and the IgA-immune complex titer, serum IgA level or blood pressure. mRNA levels in T cells obtained from patients with grade HI or IV renal histopathological findings were significantly higher than in those with grade I or II histopathology. In addition, mRNA levels in T cells obtained from patients with more than 1.0g/day proteinuria were markedly higher than those with less than 1.0 g/day proteinuria. We also studied the clinical course of 11 patients with IgAN during hospitalization. The IL-6 mRNA levels in these patients decreased gradually, as did proteinuria, after treatment. These studies suggest that abnormally regulated IL-6 mRNA expression in peripheral blood T cells may be associated with disease activity in IgAN.

ISSN:0891-6934    

DOI:10.3109/08916939309019924

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

We have recently identified in SLE sera antibodies against phosphotyrosine. They were also detected in normal sera and gammaglobulin preparations, suggesting that they belong to natural autoantibodies.In this paper, the occurrence of anti-idiotypic antibodies against anti-phosphotyrosine antibodies, in the above mentioned samples, is investigated. In order to identify these anti-idiotypic antibodies ELISA, immunoprecipitation and immunoblotting are performed.Our data demonstrate the presence of anti-idiotypic antibodies specific to anti-phosphotyrosine antibodies in SLE sera as well as in normal sera, suggesting that these anti-idiotypic antibodies are also auto-anti-idiotypic antibodies. The densitometry of immunoblots reveals significantly higher levels of anti-idiotypic antibodies in SLE sera. Based on the competition inhibition studies we conclude that some of these anti-idiotypic antibodies belong to beta/gamma type.

ISSN:0891-6934    

DOI:10.3109/08916939309019925

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

DNA binds to cell-surface proteins on human and murine leukocytes and induces secretion of the cytokine interleukin 6 (IL-6). Cell-surface DNA binding molecules have been shown to serve as target antigens for the production of autoantibodies in patients with systemic lupus erythemalosus (SLE), and in lupus-prone mice. Recent studies have demonstrated that a subset of anti-anti-DNA antibodies, isolated from patients with SLE, are idiotypically related to antibodies reactive with a cell-surface DNA binding molecule.We now report that immunization of normal mice with a murine monoclonal anti-DNA antibody induces an anti-idiotypic response which has reactivity with a cell-surface DNA binding molecule. An anti-idiotypic anti-DNA monoclonal antibody (LB17) was isolated from the spleen of an immunized mouse. This monoclonal antibody blocked the binding of DNA to murine splenocytes and mimicked the functional effect of DNA by stimulating the secretion of IL-6. These experiments provide further evidence for an idiotypic connectivity between antibodies to cell-surface DNA binding proteins and anti-DNA antibodies. It is hypothesized that this idiotypic system is part of the network of natural autoantibodies and that its perturbation may give rise to pathogenic antibodies.

ISSN:0891-6934    

DOI:10.3109/08916939309019926

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

A model of autoimmunity to rat male accessory glands (RAG) was recently developed by intraperitoneal administration of three doses of native RAG associated with liposomes. In this work we analysed the effects of gangliosides in the cellular response to RAG when they were intraperitoneally administrated prior to the second dose of liposome-associated RAG. Results show that the ganglioside treatment could modify an established DTH response. Also, gangliosides markedly reduced the number of Ia antigen-positive peritoneal exudated cells (PEC). However, they modified neither the processing of liposomes through PEC nor their viability. Moreover, we obtained cellular response by transferring PEC from immunized donors into naive receptors.

ISSN:0891-6934    

DOI:10.3109/08916939309019927

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

Experimental myasthenia gravis (EMG) was elicited in female AO rats, 8–12 weeks of age, by injection of 100μg/rat Torpedo marmorata acetylcholine receptor (AChR)-protein incorporated in CFA. Bordetella pertussis, 24×109microorganisms, rat, was injected simultaneously as additional adjuvant. Rats were sacrificed on the day of appearance of the clinical signs of EMG, and thymuses were used for histological analysis using stereologic method, and thymocyte subsets were estimated by flow cytometry. Two and three colour fluorescence was applied to determine DN (CD4-CD8-), DP (CD4+CD8+), SP-CD4+(CD4+CD8-) and SP-CD8+(CD4-CD8+) subsets, as well as thymocytes expressing TCRα/β. Rats immunized with BSA and rats injected with saline were used as controls. From 56 rats immunized with AChR-protein, 44 rats developed the disease, between day 7 and 11 after immunization. Severity of disease varied from + to + + +. Stereologic analysis of tissue sections revealed a highly significant reduction of thymic cortex and hypertrophy of medulla in EMG thymuses. Similar, but very slight changes were observed in thymuses of rats immunized with BSA. Percentages of DN, SP-CD4+, and SP-CD8+subpopulations were significantly increased, while the percentage of DP population showed a marked decrease. These preliminary data suggest an alteration of thymocyte maturational events. Whether these changes could be responsible for the initiation of autoimmunity, or are occurring as a secondary phenomenon, after EMG was already established following the injection of cross reactive antigen, is a matter for discussion.

ISSN:0891-6934    

DOI:10.3109/08916939309019928

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

For some time now, microbial agents have been implicated in the etiology of autoimmune diseases, including insulin dependent diabetes mellitus (IDDM). Recent studies, however, have revealed that exposure of genetically diabetes-susceptible animals to certain microbes or microbial agents at an early age prevents the induction and progression of disease. This suggests that microbes may act to modulate the immunological status or immune repertoire of an individual genetically programmed for IDDM away from an autoimmune response. Immunization with microbial agents at an early age may offer an important new direction for the immunotherapy of IDDM.

ISSN:0891-6934    

DOI:10.3109/08916939309019929

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

FK 506 is a new immunosuppressive agent with a similar molecular action to cyclosporin A. We have investigated immunohistochemical changes in lesional biopsies of seven patients with severe recalcitrant chronic plaque psoriasis receiving systemic FK 506 therapy. Within 4 weeks of start of treatment, there was a striking reduction in psoriasis area and severity index (mean reduction 87.4%), accompanied by marked reductions in dermal and epidermal CD4+and CD8+cells. Investigation of biopsies obtained 4–8 weeks after start of treatment revealed a significant fall in the numbers of activated mononuclear cells expressing CD25 (IL-2 receptorα-chain), HLA-DR, or CDlla (lymphocyte function-associated antigen-1, LFA-lαchain). In contrast, the number of epidermal CD1+(Langerhans) cells increased in response to FK 506 therapy. Study of leukocyte adhesion-related epitopes in active disease revealed strong expression of CD54 (intercellular adhesion molecule-1, ICAM-1) and E-selectin (previously known as endothelial leukocyte adhesion molecule-1) both on microvascular endothelial cells and of ICAM-1 on infiltrating mononuclear cells; ICAM-1 was also expressed weakly on epidermal keratinocytes. Vascular cell adhesion molecule-1 (VCAM-1) was either absent or expressed rarely on vascular endothelium. In response to FK 506 treatment, both ICAM-1 and E-selectin expression on blood vessels was reduced consistently but nevertheless persisted, even in individuals exhibiting total clearance of psoriatic lesions. The results are consistent with interference by FK 506 with production of pro-inflammatory cytokines, autocrine growth factors and the expression of adhesion molecules which are thought to regulate key interactions between keratinocytes, leukocytes and vascular endothelial cells in the pathogenesis of psoriasis.

ISSN:0891-6934    

DOI:10.3109/08916939309019930

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

Segregation analysis has suggested that the inheritance of thyroid autoantibodies (to thyroglobulin and to thyroid peroxidase) is a dominant Mendelian trait.In this study we describe an attempt to find the chromosomal location(s) of gene(s) responsible for thyroid autoantibody production. We have examined a number of restriction length polymorphisms (RFLPs) and highly polymorphic markers (mini- and microsatellite) for genetic linkage with thyroid autoantibodies using a panel of 16 families with autoimmune thyroid disease. None of the markers used in this study gave evidence of linkage, however minisatellite markers (MSI, MS31, MS32, MS43a, M851, G3) for TPO antibody, minisatellite markers (MSI, MS32, MS43a, MS51, G3) for Tg antibody, and all microsatellite markers used, provided evidence for exclusion of genetic linkage.

ISSN:0891-6934    

DOI:10.3109/08916939309019931

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

The MRL mouse strain spontaneously produces antinuclear autoantibodies that recognize DNA and the small nuclear ribonucleoprotein (snRNP) antigens. The monoclonal antibody 2.73 was derived from the lupus prone MRL/n line and is reactive with the 70K protein of the Ul snRNP particle. The epitope recognized by 2.73 was characterized by peptide and inhibition ELISA analysis. Several arginine/aspartic acid (RD) repeats of varying lengths are found in the carboxyl terminus of the 70K protein and are responsible for immunoreactivity with 2.73. We investigated the contribution of charge and found that the immunoreactivity of 2.73 and the 70K protein is specific for the RD repeats. The presentation of the epitope may also contribute to the epitopes immunoreactivity with the 2.73 mouse monoclonal autoantibody.

ISSN:0891-6934    

DOI:10.3109/08916939309019932

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

ISSN:0891-6934    

DOI:10.3109/08916939309019933

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor