ISSN:0891-6934    

DOI:10.3109/08916938909029137

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

The results reported here show that we have experimentally produced xenogeneic anti-idiotypic antibodies to rat autoantibodies specific for the renal GBM and one of its components, laminin. Cross-reactive idiotypes have been detected by anti-idiotypic antibodies (anti-Id) on autoantibodies to the GBM (anti-GBM) from rats of different strains, confirming the results obtained in other autoantibody systems. During the course of studies aimed at determining whether anti-Id were directed to the paratope of anti-GBM antibodies, we have observed the presence of anti-GBM (and anti-laminin) antibodies in rabbit sera with anti-Id. Affinity chromatography experiments suggest that anti-GBM reactions detected with our anti-Id sera may be caused by a heterogeneous combination of anti-Id. Thus, Ab2α(and/or Ab2γ, all reacting with Ab1), Ab2α(epibodies, that bind to both Ab1 and GBM) and Ab3 (similar to Abl and therefore, reacting with GBM) may be present in our anti-Id sera. It has been suggested that antibodies displaying epibody properties may be involved in the mutual regulation of autoreactive clones and represent an important component of the autoimmune process

ISSN:0891-6934    

DOI:10.3109/08916938909029138

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

Sjogren's syndrome (Ss) is an ideal model to study the pathogenesis of both autoimmunity and malignancy. It occurs as an organ specific autoimmune disease, alone or in association with almost every other autoimmune disorder, as a systemic disorder, and finally it can evolve to B-cell-lymphoid malignancy. The most consistent finding in the syndrome, the B-cell-hyperreactivity, follows the same steps of evolution. It starts as polyclonal, but not random, since the autoantibody profile correlates with the disease subgroups and the systemic manifestations and it seems to be controlled by the MHC gene composition. Further, in the systemic form of the disease it presents as a poly-oligo-mono-clonal process and ends up to monoclonal (IgMk) B-lymphoid malignancy. Studies on the T-immunoregulatory subsets and function can not explain this B-cell hyperreactivity. The initial trigger is unknown. Estrogens, known as immunoenhancers possibly promote the B-cell hyperreactivity and certain genes controlling HLA class-II MHC molecules may represent susceptibility factors for the development of the disease.The discovery of lymphokines and particularly the B-cell growth and differentiation factors as well as the rapid development of the retro-virology field may give answers pertinent to the pathogenesis of Ss and to B-cell lymphoid malignancy

ISSN:0891-6934    

DOI:10.3109/08916938909029139

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

The present paper describes a mechanism responsible for the induction of inducer-phase suppressor cells effective to suppress the autoimmune response to rat male accessory glands (RAG). In fact, we reported here that marked suppression of delayed type hypersensitivity (DTH) reaction and humoral response to chemically modified rat male accessory glands (MRAG) can be obtained when previously to be immunized with MRAG in complete Freund's adjuvant (CFA) syngeneic rats were pretreated with peritoneal cells (PC) coupled with a purified fraction of RAG (containing the autoantigen). The involvement of MRAG-specific inducer-phase suppressor cells was demonstrated by adoptive transfer experiments of spleen mononuclear cells from unresponsive donors to normal syngeneic rats 24 h prior to immunization of the recipients with MRAG-CFA.The PC used to treat the animals show a large proportion of non-specific sterase positive, Ox-41 bearing macrophage like cells. Moreover, the antigen-coupled PC able to trigger the suppressor cells showed the presence of the autoantigen of RAG on their surface. The role of the antigen presenting cells in the induction of MRAG-specific inducer-phase suppressor cells is discussed

ISSN:0891-6934    

DOI:10.3109/08916938909029140

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

The presentation of self-antigens to circulating T cells is a critical, precipitating event in the induction of autoimmune injury in parenchymal organs. Epithelia expressing these self-antigens are thought to release such moieties for reprocessing by traditional antigen-presenting cells within the lymphoid system. We now demonstrate, however, that some epithelium possess novel functional mechanisms for presenting their own antigens to a responsive, syngeneic T cell repertoire. The presentation of these self-antigens occurs in the context of MHC class II molecules and depends on CD4 associative-recognition determinants. Our findings strongly suggest that organ epithelium may directly activate cell-mediated events to produce autoimmunity through self-recognition

ISSN:0891-6934    

DOI:10.3109/08916938909029141

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

Sera from 22 untreated patients with recently diagnosed Graves' disease (GD) were screened in an immunocytochemical tissue assay for presumptive pituitary IgG autoantibodies, as defined by the presence of immunoreaction with rat and swine pituitary cell types. Forty four patients with Hashimoto's thyroiditis (HT) and 97 healthy subjects were also studied. Anti-pituitary antibodies were found in 14 of the 22 GD sera (64%). Of these, 6 sera reacted with cytoplasmic components of growth hormone (GH) cells, 3 with prolactin (PRL) cells, and 5 with both GH and PRL cells. Yet, none of the immunoreactive sera reacted with human GH, bovine PRL or TSH in dot-blot assays and absorption studies. Anti-pituitary antibodies also occurred in 4 of the 44 HT patients (9.1%) and in 9 of the 97 healthy subjects (9.2%). The frequency of sera revealing anti-pituitary antibodies was significantly higher in patients with GD compared to the groups of HT patients (P<0.00005), and healthy subjects (P<0.00005). Healthy subjects and patients with HT had a similar frequency of anti-pituitary antibodies (P = 1.0000). These data demonstrate that in thyroid autoimmune conditions antibodies reactive with cytoplasmic components of pituitary GH/PRL cells, may be present in sera from patients with GD. The pathological importance of this observation is at present unknown.

ISSN:0891-6934    

DOI:10.3109/08916938909029142

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

The stimulation of T cells is accompanied by the appearance of a number of activation antigens not found on resting T cells. We have studied the expression of one group of such antigens, the HLA class II antigens (DR, DQ and DP), following stimulation with PHA and PMA. Immunofluorescence studies using monoclonal antibodies indicated that cell surface class II determinants were detectable as little as 30 minutes following stimulation, and that their levels remained constant for about 24 hours, after which they began to increase. Studies on the molecular level demonstrated an increase in the steady state levels of mRNA for DRβby 15 minutes. Immunoblot analysis of resting T cell lysates using a monoclonal antibody reactive with DR detected a polypeptide of∼28 KDa, which agrees with the known molecular mass of the light chain of class II molecules on SDS-PAGE, suggesting that the rapid surface expression might have been due to the presence of pre-formed class II polypeptides. These results demonstrate 1) that T cells defined as resting by conventional criteria contain class II polypeptides and 2) that increases in steady state levels of class II mRNA is a very early event which precedes the initiation of DNA synthesis

ISSN:0891-6934    

DOI:10.3109/08916938909029143

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

An ELISA spot assay was used to quantitate the number of autoantibody-secreting B cells in the peripheral blood of patients with systemic lupus erythematosus. Patients with active disease had 20 fold more anti-DNA, 4 fold more anti-actin and 3 fold more anti-myosin secreting lymphocytes than controls but normal numbers of anti-cardiolipin and anti-transferrin secreting B cells. 60% of SLE patients had increased numbers of B cells reactive with multiple autoantigens. These data suggest that B cell activation in SLE may be influenced by both antigen-specific and antigen-independent factors.

ISSN:0891-6934    

DOI:10.3109/08916938909029144

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

Nicotinamide which is an inhibitor of poly (ADPR) synthetase and precursor of NAD has been observed to prevent diabetes in some experimental models possibly by protecting beta cells. To determine whether nicotinamide could cure or prevent type 1 diabetes, we administered large doses (0.5 g/Kg/d) to BB rats. When used in the 45 days following diagnosis nicotinamide failed to bring remission. As a preventive treatment, nicotinamide administered between the 40th and 90th day of age, alone or in association with desferrioxamine did not significantly lower the incidence of diabetes (23% and 30.8% respectively vs. 56.6%). When used earlier, immediately after weaning, nicotinamide did not affect the incidence of diabetes in this model (62.5%). The degree of protection was not comparable with that obtained with cyclosporin A (15% of diabetic animals). Histology study of the pancreas from the animals killed either immediately or 1 year after treatment revealed no endocrine tumor. These findings suggest that in BB rats nicotinamide has little or no effect on the course of autoimmune diabetes mellitus thus dampening the high hopes for this drug in the treatment of human diabetes.

ISSN:0891-6934    

DOI:10.3109/08916938909029145

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

Peripheral blood B lymphocytes of multiple sclerosis (MS) patients and control persons were transformed with Epstein-Barr virus. Antibody production of transformed cells against isolated human myelin was investigated by enzyme-linked immunosorbent assay (ELISA). Cells producing reactive antibodies were cloned and propagated to produce monoclonal antibodies (mAbs). These mAbs did also react with acetone fixed frozen sections of normal human white matter, as determined by indirect immunofiuorescence staining. Some of the mAbs derived from MS patients and a control person with a central nervous system cyste agglutinated liposomes made from lipids of a chloroform/methanol extract of human myelin, whereas mAbs derived from four glioma patients were negative in these tests. The reactive antibodies were investigated further using agglutination tests with liposomes made from pure auxiliary lipids (cholesterol and lecithin) or containing in addition either galactocerebroside, sulfatide or a mixture of bovine brain gangliosides. The great majority of myelin liposome agglutinating antibodies reacted with all types of liposomes, including those made from pure auxiliary lipids. Investigations by ELISA suggest that phospholipids are the reactive components, at least for some of these mAbs. Some antibodies reacted with liposomes containing galactocerebroside or sulfatide, others only with sulfatide containing liposomes. Antibodies showing these specificities were only obtained from MS patients.

ISSN:0891-6934    

DOI:10.3109/08916938909029146

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor