ISSN:0891-6934    

DOI:10.3109/08916939009002973

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

摘要:

The binding and functional activities of platelet-binding hybridoma autoantibodies from SLE patients were compared with those derived from normal individuals. Twenty-nine SLE-derived hybridoma antibodies and 20 normal-derived hybridoma antibodies were analyzed for binding to glutaraldehyde fixed platelets, dDNA and phospholipids, and for lupus anticoagulant activity. Twenty-four of the 29 SLE-derived antibodies and 9 of the 20 normal-derived antibodies showed one or more activities in these assays. Of the 24 SLE-derived antibodies, 8 (33.3%) were reactive in only one assay (monospecific), while the other 16 (66.7%) had more than one of these activities (polyspecific). In contrast, none (0%) of the 9 normal-derived antibodies with known activities were monospecific, while all 9 (100%) showed polyspecificity. Statistical analyses demonstrated that there was no correlation of anti-DNA activity with anti-platelet and most anti-phospholipid activities for the SLE-derived antibodies, and strong positive correlations between these reactivities for the normal-derived antibodies. Similarly, differences were observed in Western blotting analyses; SLE-derived antibodies bound more specifically to individual platelet proteins than normal-derived antibodies. Moreover, in chromium-51 release assays, all of the SLE-derived platelet-binding antibodies were cytotoxic to platelets, while none of the normal-derived platelet-binding antibodies showed significant cytotoxicity. Our results suggest that hybridoma platelet-binding autoantibodies derived from SLE patients exhibit greater antigen specificity and functional activity than similar antibodies derived from normal individuals.

ISSN:0891-6934    

DOI:10.3109/08916939009002974

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

摘要:

In this study we have measured the level of anti poly (ADP-ribose) antibodies in the sera of a number of patients with SLE and their relatives, patients with a wide variety of other autoimmune and infectious diseases, and a group of normal healthy controls.It was found that these antibodies were not disease specific but were present in nine out of thirteen groups tested in significant numbers.The levels of anti poly (ADP-ribose) antibodies and anti DNA antibodies in SLE patients bled serially were also measured. The level of these antibodies fluctuated in parallel in many of these patients, although the anti poly (ADP-ribose) antibodies reflected disease activity more accurately in some.

ISSN:0891-6934    

DOI:10.3109/08916939009002975

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

摘要:

HLA-DP+T cells in peripheral blood from 23 patients with systemic lupus erythematosus (SLE) were examined using two-colour flow cytometry analysis. A marked increase of HLA-DP+T cells was observed in patients with SLE (20.5–98.7%; 59.8±20.8%) in comparison to normal subjects (1.3–20.6%; 11.1±7.2%), and the ratio of these cells greatly exceeded that of the HLA-DR+ T cells (6.5–49.1%; 21.5±12.7%). This high frequency of HLA-DP+ T cells in patients with active SLE decreased with predonisolone therapy. When the lymphocytes from normal subjects were stimulated with PHAin vitro, HLA-DP+T cells increased from 1.8 to 59.2%. Therefore, it appears that the HLA-DP antigen expression on T cells is a practical marker for monitoring changes in the proportion of activated T cells in patients with SLE during the course of therapy.

ISSN:0891-6934    

DOI:10.3109/08916939009002976

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

摘要:

The directin vitroeffect of interleukin-6 (IL-6) on pancreatic beta-cells was studied using isolated Lewis rat islets (25/ml/well) precultured for 7 days and then incubated with or without human recombinant 1L-6 (rIL-6) or purified human natural IL-6 (nlL-6). Both sources of IL-6 stimulated insulin secretion over a period of 6 days (P<0.01). whereas the levels of insulin within the islets were unaffected. At concentrations above 1.5ng/ml, rIL-6 almost doubled the content of insulin in the supernatants. At an intermediate concentration, 0.5ng/ml, rIL-6 preserved insulin secretion by islets cocultured with 2ng/ml of human recombinant interleukin lβ(rIL-Iβ) which otherwise inhibited insulin secretion to 60% of islets cultured in medium alone. Electron microscopic studies showed that rIL-6, 1.5ng/ml, caused beta-cell specific degenerative changes similar to those previously described after treatment with IL-1β; i.e. appearance of opaque intracytoplasmic bodies, autophage vacuoles and signs of mitochondrial degeneration. We conclude that human IL-6 stimulates insulin production and secretionin virroand induces similar ultrastructural changes in beta-cells as does IL-Iβ. IL-6 may be an endogenous mediator of some of the effects on beta-cells ascribed to IL-1.

ISSN:0891-6934    

DOI:10.3109/08916939009002977

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

摘要:

The injection of lymphoid cells from adult Ipr mice into normal and athymic congenic mice does not transfer the Ipr (lymphoproliferation) syndrome1, We studied whether this phenomenon could be due to abnormal homing.The lymphoid cells from Ipr donors do not show a marked deficiency of migration to lymphoid organs in comparison with cells from Wild donors and a T-cell lymphona BL/VL3. The lymphoid organs of Ipr recipients do not show an intrinsic abnormality as homing sites for lymphoid cells.The data reveal some minor migration preferences: the Ipr cells migrate better than Wild cells into Ipr lymph nodes (including athymic Ipr hosts), whereas Wild cells migrate slightly better than Ipr cells into Wild lymph nodes.In spite of such minor preferences, Wild cells can efficiently migrate into Ipr lymphoid organs, as well as Ipr cells into Wild lymphoid organs. Thus, the lack of transfer of lymphadenopathy in Wild recipients cannot be attributed to an alteration of Ipr cell homing.

ISSN:0891-6934    

DOI:10.3109/08916939009002978

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

摘要:

We have studied the clinical significance of cy to toxic antibodies against human eye muscle cells in patients with thyroid-associated ophthalmopathy (TAO). Eye muscle reactive antibodies were measured in an antibody-dependent cell-mediated cytotoxity (ADCC) assay.A positive test was defined as % specific Iysis greater than the upper limit of normal, taken as the mean plus two standard deviations for normal subjects tested concurrently. As parameters of the severity of the ophthalmopathy we measured the degree of proptosis (mm), level of intraocular pressure (IOP) (mmHg) and American Thyroid Association classes (0–6). ADCC tests were positive in 21 out of 42 patients with TAO and in 8 out of 14 patients with Graves' disease without evident eye disease but in none of 12 normal subjects tested. In patients with TAO mean (±SE) IOP was significantly greater than that in patients with Graves' disease without apparent eye involvement for the primary position and for all gaze positions. There were significant positive correlations between levels of eye muscle reactive cytotoxic antibodies and the severity of the eye disease quantitated as American Thyroid Association classes 0–6, the IOP in the primary position and on downgaze, but not with the degree of proptosis. These results suggest that cytotoxic antibodies, as detected in ADCC, may play a role in the eye muscle damage of TAO and that their measurement may provide a useful clinical test.

ISSN:0891-6934    

DOI:10.3109/08916939009002979

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

ISSN:0891-6934    

DOI:10.3109/08916939009002980

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

ISSN:0891-6934    

DOI:10.3109/08916939009002981

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

ISSN:0891-6934    

DOI:10.3109/08916939009002982

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor