摘要:

In this paper we report that intraperitoneal administration of type II collagen in a soluble form protects DBA/1 mice against collagen-induced arthritis (CIA) on subsequent arthritogenic challenge with soluble type II collagen in adjuvant. The degree of arthritis suppression, which is expressed in terms of reduced incidence of arthritis, delayed onset and reduced anti-collagen antibody titres, depends on the dose and timing of the pre-immunization collagen injection. In order to elucidate the rôles of CD4+and CD8+T lymphocyte subsets in arthritis resistance we administered monoclonal antibodies (mAb) to these antigenic determinants around the time of immunization with soluble type II collagen. Anti-CD4 mAb caused abrogation of arthritis resistance while anti-CD8 mAb was less effective. However, administration of anti-CD8 mAb two weeks after pre-immunization with soluble collagen was very effective in reversing arthritis resistance. From these findings we conclude that CD4+and CD8+T cells are involved in resistance to arthritis though the relative importance of each subset changes during the course of the process leading to resistance to CIA.

ISSN:0891-6934    

DOI:10.3109/08916938909014700

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

This study describes an assay for the detection of cytotoxicity for thyroid cells in serum of patients with autoimmune thyroiditis. Quantative measurement may be performed by DNA or [3H] leucine incorporation determinations. The cytotoxic effect is localized in the gamma-globulin fraction, and is complement-mediated. It is thyroid specific i.e. it is not observed with fibroblasts and patients with other autoimmune diseases (patients with lupus erythematosis or glomerulonephritis) do not have cytotoxic antibodies directed against thyroid cells. The thyroid cytotoxicity is related to the presence of antimicrosomal antibodies and the effect of circulating antibodies is inhibited by human thyroid peroxidase. These results strengthen the possible implication of circulating antithyroid peroxidase antibodies in thyroid damage observed in autoimmune thyroiditis.

ISSN:0891-6934    

DOI:10.3109/08916938909014701

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

In both thyroid autoimmune diseases Graves' and Hashimoto's thyroiditis, the epithelial thyroid follicular cells (TFC) have been shown to express HLA class II molecules, and can restimulate autoreactive T cells cloned from the diseased tissue. This aberrant class II expression is important in the mechanism of perpetuation of the disease process, therefore we have compared the effect of interferonγ(IFNγ) and tumour necrosis factor (TNFα) on the HLA-DRαmRNA expression of thyroid follicular cells derived from Graves' disease (GD) and a non autoimmune disease, non toxic goitre (NTG).Our results indicate that TNFαsynergises with IFNγin the induction of HLA class II mRNA. There was no consistent difference in DRαmRNA expression between the GD and NTG thyroid follicular cell preparations in response to induction by a combination of these lymphokines at various concentrations. Our data suggest that the differences in the level of expression of class II molecules observedin vivoin Graves' disease and non toxic goitre, which is much higher in the former, is probably due to local release of lymphokines by infiltrating T lymphocytes, although other factors may be involved.

ISSN:0891-6934    

DOI:10.3109/08916938909014702

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

T lymphocytes present in thyroid infiltrates of 6 patients with Hashimoto's thyroiditis (HT) and of 4 patients with Graves' disease (GD) were analyzed at clonal level and their profiles of mitogen-induced lymphokine secretion were characterized. Production of interleukin-2 (IL-2), interleukin-4 (IL-4), interferon-gamma (IFN-γ) was measured in culture supernatants of a total number of 332 T cell clones (TCC) from HT, of 269 TCC from GD infiltrates and of 266 control TCC derived from normal lymphoid tissues. No significant difference was found in the ability to produce IL-2 between TCC from HT or GD infiltrates and control TCC. The proportion of HT-or GD-derived TCC able to produce IL-4 was extremely low (4 and 5%, respectively) in comparison with controls (19%). In contrast, the proportion of interferon-gamma (IFN-γ)-producing (IFN-P) TCC derived from either HT (87%) or GD (80%) infiltrates was much higher (p<0.0005) than that found in controls (59%). In addition, most of IFN-P TCC from either HT or GD usually released higher amounts (p<0.002) of IFN-γthan did control clones. No significant difference was found between GD infiltrates and controls in the proportions of TCC able to secrete TNF-α(39% and 47%, respectively), whereas the proportion of TNF-α-producing (TNF-P) TCC derived from HT (78%) was significantly higher (p<0.0001). In addition, most of both CD8 and CD4 TCC from HT released higher amounts of TNF-αthan did TNF-P clones from controls or GD. These data suggest that T cells present in autoimmune thyroid infiltrates share a number of functions, such as high production of IFN-γ, but differ with regard to their ability to secrete TNF-α, which is peculiar of most T cells present in the thyroid of HT patients.

ISSN:0891-6934    

DOI:10.3109/08916938909014703

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

The binding of human lupus anti-DNA antibodies and murine anti-mycobacterial antibodies to human cortical brain tissue sections was assessed by the indirect immunofluorescent technique.Prior adsorption of the reactive monoclonal antibodies on nuclear extracts, ss-DNA, synthetic polynucleotide polymers, histones, mycobacterial glycolipids, and bovine brain extracts abrogated the monoclonal antibodies' binding to the brain. Intermediate blocking activity was conferred also by ribonucleic components as RNP, Ro(SSA), and La(SSB). Specificity to neuronal tissue was demonstrated by failure of non-neuronal tissue extracts to abolish antibodies' reaction to the cortical tissue sections in competition assays. The anti-TB and anti-DNA antibodies seemed to compete on their binding to a common neuronal membranal epitope. These results indicate that mycobacteria share antigens with DNA and human brain tissue.Furthermore, these data support the concept that anti-DNA antibodies may play a pathogenetic role in SLE patients with neuropsychiatric involvement via crossing of a“leaky”blood brain barrier and attachment to brain tissue components.

ISSN:0891-6934    

DOI:10.3109/08916938909014704

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

摘要:

Mitochondrial antigens were purified from rat liver and characterized by immunoblotting. Sera from 19 well defined patients with primary biliary cirrhosis (PBC) reacted with two mitochondrial polypeptides of 68 Kd and 45 Kd. Antibodies to these antigens were not detected in any of the sera of patients with cirrhosis of the liver, chronic active hepatitis or other autoimmune diseases. The two polypeptides were derived from the soluble fraction of the mitochrondrial matrix.An enzyme-linked immunosorbent assay (ELISA) employing these rat liver mitochondrial antigens is described. Positive results were obtained with all except one PBC sera (95%), five out of 47 patients with cirrhosis (11%), one out of 20 patients with chronic active hepatitis (5%), and two out of 19 patients with various autoimmune disorders (11%). The titers detected in PBC were markedly higher than those recorded in patients with other liver and autoimmune diseases. Strong correlation was found between immunoblotting and the ELISA in determining antimitochondrial antibodies.The ELISA presented is easily performed and seems to be a useful diagnostic tool for antimitochondrial antibodies in patients with PBC.

ISSN:0891-6934    

DOI:10.3109/08916938909014705

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

ISSN:0891-6934    

DOI:10.3109/08916938909014706

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor

ISSN:0891-6934    

DOI:10.3109/08916938909014707

出版商:Taylor&Francis    

年代:1989

数据来源: Taylor