摘要:

AbstractMeasles virus can replicate in cultures of both infantile and fetal human thymic epithelial cells. Virus‐induced cytopathology including syncytium formation was first evident around 24 hr after viral inoculation of these cultures. At the same time, the cultures began to lose their characteristic thymus‐like organizational structure. Viral antigens were detected in infected cells by indirect immunofluorescence, and the presence of progeny virions was demonstrated in culture flu

ISSN:0146-6615    

DOI:10.1002/jmv.1890270112

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractThe specificity and neutralizing activity of antibodies against the major herpes simplex virus type 1 (HSV‐1) glycoproteins were tested in serum samples of patients with a history of HSV‐1 infection. By preabsorption of sera to preparations of native and denatured HSV‐1 proteins, followed by immunoblotting and microneutralization, it was shown that the majority of neutralizing antibodies are directed against denaturation‐sensitive epitopes. Furthermore, preabsorption of sera to proteins of viral ts and deletion mutants revealed that antibodies specific for gB, gC, and gE had a low neutralizing activity. These results suggest a major role of anti‐gD in neutralization of viral infectivity. In addition, it was shown that antibodies directed against the gB monomer were distinct from antibodies against the gB homodimers. The latter, however, did not reveal any measurable neutralizing

ISSN:0146-6615    

DOI:10.1002/jmv.1890270113

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractCytolytic recognition of CMV‐infected syngeneic fetal guinea pig cells by maternal peripheral blood mononuclear cells (PBMC) was suppressed late in pregnancies of uninfected guinea pig breeders with50% fetal wastage exhibited only partial suppression of cytolytic activity against CMV‐infected fetal cells. Primary CMV infection of dams extending into early pregnancy induced augmented cytolysis of CMV‐infected fetal cells, but not MA104 NK cell targets, throughout gestation and resulted in 70% loss of conceptus. Decreased suppression of cytolytic activity against CMV‐infected fetal cells in uninfected pregnancy was also associated with runting of newborn pups, which was not as severe as that observed in congenitally CMV‐exposed or CMV‐infected pups. Congenitally infected pups were affected more than their exposed but uninfected litter mates. Lack of suppression of cytolysis of CMV‐infected syngeneic fetal cells, whether spontaneous or CMV‐infection‐induced, appears to be associated with poor

ISSN:0146-6615    

DOI:10.1002/jmv.1890270114

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractTen sera from healthy blood donors positive by enzyme‐linked immunoadsorbent assay (ELISA) were studied by immunoblot assay using natural and recombinant proteins. They interacted only with p17 or p24 proteins but were nonreactive with a recombinant protein (RP 50), which carries antigenic determinants to p17 and p24. Reactions were not blocked by preincubation of sera with genetically engineered p17 and p24 or purified viral p24, indicating that some new epitopes were formed during the Western blot procedure. Recombinantgag‐encoded protein is required for confirmation of human immunodeficiency virus (HIV) seropositiv

ISSN:0146-6615    

DOI:10.1002/jmv.1890270115

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractIn a patient who followed the typical clinical course of fulminant hepatitis attributable to “sporadic” non‐A, non‐B (NANB) hepatitis and who finally received treatment by orthotopic liver grafting, three, apparently separate, viruslike agents (26, 45, and 80 nm) and cytoplasmic, reticular tubular structures (CTS) were identified in collapsed and regenerating areas of liver using electron microscopy. The 80‐nm particles present within vacuoles, together with the finding of intranuclear rods in association with the smaller particles (26 nm), are similar to those found in the nuclei of cells infected with several different arboviruses. The third type of particle, existing as 45‐nm spheres and rods, is similar in morphology only to some form of polyoma virus, which, hitherto, has not been reported as affecting the liver. Unlike typical polyoma virus, replication of the virus “cores” (25–26 nm) was extranuclear and appeared to be occurring in vacuoles. Although analysis for serological markers against a representative panel for arboviruses, flaviviruses, phleboviruses, arenavirus, and nairovirus was negative, an insect vector was implicated in the

ISSN:0146-6615    

DOI:10.1002/jmv.1890270116

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

ISSN:0146-6615    

DOI:10.1002/jmv.1890270101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY