ISSN:0025-729X    

DOI:10.5694/j.1326-5377.1993.tb137690.x

出版商:Wiley    

年代:1993

数据来源: WILEY

ISSN:0025-729X    

DOI:10.5694/j.1326-5377.1993.tb137691.x

出版商:Wiley    

年代:1993

数据来源: WILEY

ISSN:0025-729X    

DOI:10.5694/j.1326-5377.1993.tb137692.x

出版商:Wiley    

年代:1993

数据来源: WILEY

摘要:

ObjectiveHuman T‐lymphotropic virus type I (HTLV‐I) is known to be endemic among Northern Territory (NT) Aborigines, therefore evidence was sought of HTLV‐I infection in NT blood donors.DesignSamples were screened for HTLV‐I antibodies using the Serodia HTLV‐I particle‐agglutination assay. Repeatedly reactive sera were tested by western blot. Viro‐ logical and molecular investigations were also performed.SeraAliquots from all 11 121 blood donations collected between June 1991 and August 1992.ResultsFour (0.036% of total) blood donations, each from different donors, were repeatedly reactive by particle‐agglutination assay. One (0.009%) sample, from a 52‐year‐old non‐Aboriginal man with no verified risk factors, was confirmed as HTLV‐I seropositive by western blot. A viral isolate and a 431 base pair polymerase chain reaction product from theenvgene were obtained from a culture of his peripheral blood mononuclear cells. Sequencing of the polymerase chain reaction product demonstrates that this isolate is a prototype strain and not the variant identified among Aborigines. The remaining three repeatedly reactive donors, including the positive donor's wife, were western blot indeterminate.ConclusionsThere is a low prevalence of HTLV‐I carriage among blood donors in the NT, and presumably in other States. However, most repeatedly reactive donations prove to be western blot indeterminate, therefore additional tests are required to detect or exclude HTLV‐I infection. Although universal screening of donations would virtually eliminate HTLV‐I transmission by transfusion, it has disadvantages, including financial cost.

ISSN:0025-729X    

DOI:10.5694/j.1326-5377.1993.tb137693.x

出版商:Wiley    

年代:1993

数据来源: WILEY

ISSN:0025-729X    

DOI:10.5694/j.1326-5377.1993.tb121989.x

出版商:Wiley    

年代:1993

数据来源: WILEY

摘要:

ObjectiveTo survey the Aboriginal community of the Northern Territory for antibodies to human T‐lymphotropic virus type I (HTLV‐I) and to describe the distribution of the virus.DesignA sero‐epidemiological study using the Serodia particle‐agglutination assay, indirect immunofluorescence and western blot. Evidence of HTLV‐I‐related diseases was sought through clinicians, and by searching the cancer register and medical records.SeraSamples from 1897 Aborigines, including 1569 sera received by the Royal Darwin Hospital Pathology Department for syphilis serology between March and July 1988. Most of the specimens were from public health surveys and antenatal screening.ResultsNinety‐four samples (5.0%) were positive by the particle‐agglutination assay method but only 36 (1.9%) were positive by both particle‐agglutination assay and indirect immunofluorescence. After confirmation by western blot, the seroprevalence of HTLV‐I was 1.7% (95% confidence interval, 1.2‐2.3%). Western blot positivity was higher in samples from the "Cattle Country" and Alice Springs regions (i.e., 4.7% and 13.9% respectively).ConclusionHTLV‐I is endemic among Aborigines in inland Australia. These serological findings are supported by the recognition of two cases of adult T‐cell leukaemia/lymphoma in this population.

ISSN:0025-729X    

DOI:10.5694/j.1326-5377.1993.tb137694.x

出版商:Wiley    

年代:1993

数据来源: WILEY

ISSN:0025-729X    

DOI:10.5694/j.1326-5377.1993.tb121991.x

出版商:Wiley    

年代:1993

数据来源: WILEY

ISSN:0025-729X    

DOI:10.5694/j.1326-5377.1993.tb121992.x

出版商:Wiley    

年代:1993

数据来源: WILEY

ISSN:0025-729X    

DOI:10.5694/j.1326-5377.1993.tb121993.x

出版商:Wiley    

年代:1993

数据来源: WILEY

ISSN:0025-729X    

DOI:10.5694/j.1326-5377.1993.tb121994.x

出版商:Wiley    

年代:1993

数据来源: WILEY