摘要:

We have analysed the postnatal development of the nuclear sizes of the granular cells of the dentate gyrus in 5- to 190-day-old male mice. The study was performed in three topographic levels: rostral, intermediate and caudal. Three subdivisions were analysed in each level: suprapyramidal blade, infrapyramidal blade and the transition between them, the angular zone. Additionally, each of these subdivisions was measured in its external and internal layer, separately. Three gradients of postnatal karyometric development can be described: external-to-internal, suprapyramidal-to-infrapyramidal, and caudal-to-rostral, indicating that the external, suprapyramidal and caudal cells show higher karyometric sizes than the other subdivisions. These gradients are related to the ontogenetic gradients of these neurons.

ISSN:1422-6405    

DOI:10.1159/000147300

出版商:S. Karger AG    

年代:1992

数据来源: Karger

摘要:

The immunohistochemical localization of proteins Gi1 (plus Gi3). Gi2 and Go was studied in the olfactory epithelium and the main olfactory bulb of rats, using purified antibodies to the respective α subunits and βγ subunits of these G proteins. In the olfactory epithelium, only a restricted population of olfactory cells was immunopositive for Gi2α, but others were not. The immunoreactivity for Gi1α/Gi3α was not observed. The olfactory epithelium was immunopositive for both Goα and βγ, but its apical surface was immunopositive only for βγ. In the main olfactory bulb, all layers were intensely immunopositive for Goα and βγ but weakly for Gi2α. In contrast to the negative or weak immunostainings in the olfactory nerve fiber layer and glomeruli, the molecular and the internal granular layers were intensely immunopositive for Gi1α/Gi3α. These findings suggest the functional difference among Gi1α/Gi3, Gi2 and Go in the signal transduction in the

ISSN:1422-6405    

DOI:10.1159/000147301

出版商:S. Karger AG    

年代:1992

数据来源: Karger

摘要:

The microvascularization of the bile duct was studied in 20 adult guinea pigs (Cavia porcellus) using microvascular corrosion casts in the scanning electron microscope. The main supplying and draining vessels are located in the adventitial layer; from there they form the subepithelial capillary network. The micro-angioarchitecture of the common bile duct is reminiscent of that of the cystic and hepatic ducts. The diameter of these ducts is 1,000 µm on average. The micro-vascular arrangement in the bile duct suggests a probable involvement of the capillaries in the absorption of bile hormones

ISSN:1422-6405    

DOI:10.1159/000147302

出版商:S. Karger AG    

年代:1992

数据来源: Karger

摘要:

Computer three-dimensional reconstruction of serial sections is currently an active area of research. In this paper we combine computer graphics, image processing and biomedical techniques to reconstruct a stereo model of intraglandular lymphatic vessels, veins, arteries and ducts from serial microsections of the human submandibular gland.

ISSN:1422-6405    

DOI:10.1159/000147303

出版商:S. Karger AG    

年代:1992

数据来源: Karger

摘要:

A sperm antigen corresponding to baboon sperm monoclonal antibody 1A9 was localized in the testis and ejaculated sperm in this animal, using the immunofluorescence technique and immunogold labelling. Immunohistochemical studies of the baboon testis showed that the antigenic determinant was localized in the late spermatid cells and spermatozoa close to the seminiferous tubules. Immuno-fluorescence studies indicate that the protein was localized on the acrosome region of ejaculated baboon sperm. At the electron-microscopic level, gold particles indicative of the presence of this determinant recognized by 1A9 monoclonal antibody were detected on the inner acrosomal region of ejaculated baboon sperm.

ISSN:1422-6405    

DOI:10.1159/000147304

出版商:S. Karger AG    

年代:1992

数据来源: Karger

摘要:

The ultrastructure of the parathyroid glands of phosphate-treated golden hamsters exposed to a 5-G environment was studied. In the phosphate-treated animals exposed to a hypergravity environment, the Golgi complexes associated with numerous prosecretory granules, and the enlarged intercellular spaces containing floccular or finely particulate material showed a significant increase compared to those of the control, centrifuged, and phosphate-treated groups, and the cisternae of the granular endoplasmic reticulum showed a significant increase compared to those of the control and phosphate-treated groups. In addition, numerous secretory granules were situated close to the plasma membrane of chief cells in the phosphate-treated animals exposed to a hypergravity environment. These findings suggest that the synthesis, and to a greater extent the release of secretory granules may be markedly stimulated, in the parathyroid glands of phosphate-treated animals exposed to a hypergravity environment.

ISSN:1422-6405    

DOI:10.1159/000147305

出版商:S. Karger AG    

年代:1992

数据来源: Karger