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1. |
Ultrastructure of the Endolymphatic Sac in the Mouse |
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Cells Tissues Organs,
Volume 141,
Issue 3,
1991,
Page 193-198
H. Furuta,
N. Mori,
M. Fujita,
S. Sakai,
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摘要:
The ultrastructure of the endolymphatic sac (ES) in the mouse was examined by light and electron microscopy. This organ was divided into three parts: proximal, intermediate and distal. In the proximal portion of the ES, the epithelium consisted of thin squamous cells. The epithelial cells had acquired basolateral processes, numerous small vesicles and well-developed Golgi apparatus. In the intermediate portion, the epithelium consisted of columnar or cuboidal cells. The epithelial cells could be classified into two types: type I and type II. The type I cells had abundant microvilli, pinocytotic vesicles, vacuoles, multivesicular bodies, lysosomes and mitochondria. The type II cells had fewer numbers of these organelles. A few free-floating cells could be observed in the lumen of this intermediate portion, most of which were macrophages. In the distal portion, the epithelium consisted of squamous or cuboidal cells. The epithelial cells had a few cytoplasmic organelles. In the ultrastructural study, each portion of the mouse ES was found to have a very distinct morphological feature. It was suggested that each of these three portions has a different function.
ISSN:1422-6405
DOI:10.1159/000147121
出版商:S. Karger AG
年代:1991
数据来源: Karger
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2. |
Morphology and Histochemistry of the Myotendineal Junction of the Rat Calf Muscles |
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Cells Tissues Organs,
Volume 141,
Issue 3,
1991,
Page 199-205
M. Kvist,
L. Jozsa,
P. Kannus,
J. Isola,
T. Vieno,
M. Järvinen,
M. Lehto,
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摘要:
The macromolecular composition and morphometry of the myotendineal junction (MTJ) of slow-twitch (type 1) and fast-twitch (type 2) muscle fibers were studied in gastrocnemius-soleus-Achilles unit of the rat. Proteoglycans and glycosaminoglycans, type III collagen, fibronectin and laminin could be detected at the myotendineal junction. Due to the membrane folding finger-like processes were seen at the MTJ. The processes of type 1 fibers were greater in size. However, due to the subdivisions the processes of type 2 muscle fibers had a significantly greater surface length per muscle cell diameter than type 1 fibers. The myotendineal endings of both fiber types had a characteristic basal lamina, which was about three times thicker than in the longitudinal site of the same muscle cells. The basal lamina of type 1 fibers at the MTJ was significantly thicker than that of type 2 fibers.
ISSN:1422-6405
DOI:10.1159/000147122
出版商:S. Karger AG
年代:1991
数据来源: Karger
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3. |
A Bilateral Maxillofacial Trunk in Man: An Extraordinary Anomaly of the Carotid System of Arteries |
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Cells Tissues Organs,
Volume 141,
Issue 3,
1991,
Page 206-211
M.L. Pretterklieber,
E.B. Krammer,
R. Mayr,
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摘要:
In a Caucasian male, the maxillary artery (M) bilaterally arose with the facial artery anteromedially from the external carotid artery. On the right side, the M entered the infratemporal fossa between the neck of the mandible and the medial pterygoid muscle, wereas the left M pierced the medial pterygoid muscle, first being covered by the muscle and the angle of the mandible. On both sides, the M ran deep to the inferior head of the lateral pterygoid muscle and the buccal nerve. The right M lay deep to the inferior alveolar, but superficial to the lingual nerve, whereas the left inferior alveolar and lingual nerves had formed two roots, thus encircling the left M. The ascending palatine artery was replaced on both sides by palatine branches of theascending pharyngeal artery. Since a bilaterial maxillofacial trunk with topographical relations as described herein has not been previously reported in man, the embryology and comparative anatomy of this variation are discussed.
ISSN:1422-6405
DOI:10.1159/000147123
出版商:S. Karger AG
年代:1991
数据来源: Karger
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4. |
Ultrastructural Study on a Variety of Non-Neural Cells Immunoreactive for Nerve Growth Factor Receptor in Developing Rats |
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Cells Tissues Organs,
Volume 141,
Issue 3,
1991,
Page 212-219
O. Amano,
H. Abe,
H. Kondo,
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摘要:
The occurrence of nerve growth factor receptor (NGFR) immunoreactivity was newly demonstrated in mesenchymal cells of lung bud and lateral palatine processes. Nerve fibers and enclosing Schwann cells with or without immunoreaction were present only in the periphery of the immunoreactive mesenchymal cell aggregation but were not found within them. The NGFR-immunoreactive cells in immature skeletal muscles were revealed by immunoelectron microscopy to be perimysial cells without myofilament bundles, but not myoblastic cells. NGFR immunoreactivity was detected in noninnervated epithelial cells performing the invagination such as epithelial cells of the renal glomerulus and the lens placode. The immunoreactivity was also expressed in the innermost cells of the theca cell layer of the postnatal ovary and they contained no lipid droplets. In postnatal lymphatic tissues, NGFR-immunoreactive cells were identified as the interdigitating cells which were located in the internodular and deep cortex of the lymph nodes, in the marginal zone of the splenic white pulp, and in the medulla of the thymus. These findings suggest a potentially more widespread involvement of nerve growth factor and NGFR in the program of ontogeny than expected.
ISSN:1422-6405
DOI:10.1159/000147124
出版商:S. Karger AG
年代:1991
数据来源: Karger
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5. |
Microcirculation of the Extra-Ocular Muscles of Rats |
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Cells Tissues Organs,
Volume 141,
Issue 3,
1991,
Page 220-224
L. Pannarale,
P. Onori,
E. Gaudio,
M. Ripani,
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摘要:
Specific researches, employing corrosion casts, were performed on different skeletal muscles, but not on extra-ocular muscles (EOMs). The microvascular bed of EOMs was studied by the corrosion cast technique in the rat. Two histologically and physiologically different layers were present in the EOMs. On the whole the capillary pattern of EOMs was less dense than in the other skeletal muscles. The EOM orbital layer turned out to have a higher number of transverse anastomoses and tuning-fork divisions than the global layer had. These different microcirculatory patterns can be related with the physiological function and the anatomical situation of the EOMs.
ISSN:1422-6405
DOI:10.1159/000147125
出版商:S. Karger AG
年代:1991
数据来源: Karger
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6. |
Postnatal Development of the Interstitial Cells (Palisade Cells) of the Pars Intermedia in the Cat Pituitary Gland. An Immunocytochemical and Ultrastructural Study |
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Cells Tissues Organs,
Volume 141,
Issue 3,
1991,
Page 225-232
J.F. Marin,
J. Boya,
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摘要:
The postnatal development of the interstitial agranulated cells (so-called palisade cells) of the pars intermedia in the cat was investigated immunocytochemically and at the ultrastructural level. Since the first postnatal days a strong vimentin immunoreactivity and a weaker S-100 protein immunoreactivity were detected in the marginal cells lining the pituitary cleft and in the interstitial bipolar cells located within the pars intermedia. No glial fibrillary acidic protein cells have been found in the pars intermedia of any of the animals studied. This immunocytochemical pattern was maintained throughout the postnatal development. Ultrastructurally these cells showed a vast number of cytoplasmic filaments and well-developed junctional complexes. Secretory granules were never seen. In older animals they lined microcavities and microchannels where they project microvilli and present pinocytotic vesicles on their apical surface. No transitional forms between these cells and granulated secretory cells were found. There is a large number of axons and synaptic endings in contact with the granulated secretory cells. From our findings we guess that palisade cells are not a glial derivative, but they may share a common origin with secretory granulated cells.
ISSN:1422-6405
DOI:10.1159/000147126
出版商:S. Karger AG
年代:1991
数据来源: Karger
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7. |
Anatomische Aspekte der Arteria inferior posterior cerebelli für die Chirurgie der hinteren Schädelgrube |
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Cells Tissues Organs,
Volume 141,
Issue 3,
1991,
Page 233-238
T. Koperna,
G. Kopriva,
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摘要:
As the arteria inferior posterior cerebelli sometimes causes surprises of grave consequence at surgical approaches, its origin and course had to be described in detail. For this aim, we especially considered the local relationships of this artery with the medulla oblongata and the cranial nerves of the posterior fossa. Branches of the a. inferior posterior cerebelli supplying the medulla oblongata have been noticed in relation to the arterial supply of the medulla oblongata by corresponding vessels and the aa. spinales lateralis and posterior with special reference to all the possible collateralizations.
ISSN:1422-6405
DOI:10.1159/000147127
出版商:S. Karger AG
年代:1991
数据来源: Karger
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8. |
Relationships between Bone and Skin Atrophies during Aging |
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Cells Tissues Organs,
Volume 141,
Issue 3,
1991,
Page 239-244
D. Chappard,
Ch. Alexandre,
J.-M. Robert,
G. Riffat,
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摘要:
Bone mineral density (BMD) was measured in 133 female subjects (age: 61.7 ± 16.3 years) by dual energy X-ray absorptiometry (DEXA). Vertebral bone mineral density (BMD; L1–L4) and BMD of the whole upper femoral extremity were taken into account. In addition, skinfold thickness was measured with a callipers on the dorsum of the nondominant hand. A significant negative correlation was found between skinfold thickness and age (r = –0.623, p < 0.0001). Both vertebral and femoral BMD decreased with age and the slopes were similar to those observed by other authors. Skinfold thickness was significantly correlated with vertebral (r = 0.364, p < 0.0001) and femoral BMD (r = 0.486 p < 0.0001). Skin and bone are connective tissues whose extracellular matrix mainly contains type I collagen. It is postulated that age-related skin atrophy and bone atrophy have a common genetic mechanism. Skinfold thickness measurement may help in defining the women at risk for osteoporotic bone fractures who should be referred for a DEXA examina
ISSN:1422-6405
DOI:10.1159/000147128
出版商:S. Karger AG
年代:1991
数据来源: Karger
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9. |
Changes in Lectin-Binding Patterns during Late Fetal Development of the Rat Colon |
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Cells Tissues Organs,
Volume 141,
Issue 3,
1991,
Page 245-250
R.R. Hill,
G. de Bruyn,
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摘要:
Lectin-binding and histochemical studies were integrated with a morphological description of colon development in rat fetuses to determine whether changes in glycoprotein sugars could be identified with stages of colon organogenesis. At 16 days gestation the colon consisted of a minute lumen surrounded by 3–5 layers of cuboidal stratified epithelium, a basement membrane and undifferentiated mesenchyme. As development proceeded, epithelial spaces eventually fused with the main lumen, and fingers of mesenchyme, with a basement membrane and epithelial mantle, formed the walls of crypts lined with simple to stratified columnar cells. Goblet cells and mucin production appeared only on the 20th-21st day of gestation. Mesenchyme differentiation to a circular muscle band, the prospective tunica muscularis, occurred on days 17–18, and vascularization of the lamina propria was first detected on day 19. Wheat germ agglutinin (WGA) lectin bound avidly to sugar residues in the mesenchyme matrix from day 16 but weakened as the tissue differentiated, particularly into smooth muscle. Alcian blue staining of the matrix, also weakening with time, confirmed the sialic acid nature of binding sites for WGA on matrix acid mucopolysaccharides. Under controlled conditions, WGA also detected N-acetylglucosamine-binding sites on brush borders as they developed on the apical surface of primitive enterocytes. Ulex lectin bound to fucose residues of Alcian-blue-positive, PAS-positive mucin from the first appearance of goblet cells. Concanavalin A and glycine max lectins bound only to blood group substances on both nucleated red cells between days 16 and 19 and anucleate red cells from day 19. The study shows that significant changes occur in the structure of glycosylated molecules in mesenchyme and epithelium, during the brief period of intense organogenesis of the rodent co
ISSN:1422-6405
DOI:10.1159/000147129
出版商:S. Karger AG
年代:1991
数据来源: Karger
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10. |
Growth Allometry of the Myocardium in Human Embryos (from Stages 15 to 23) |
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Cells Tissues Organs,
Volume 141,
Issue 3,
1991,
Page 251-256
C.A. Mandarim-de-Lacerda,
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摘要:
The relative growth of the myocardium was studied in 27 staged human embryos (Carnegie stages). The volume of the myocardium was determined for each embryo according to Cavalieri’s principle (by using point-counting planimetry to determine the area of the profiles of the myocardium). The volume of the myocardium (variable Y) was correlated to embryonic crown-rump length (variable X in millimeters) and age (in days). The bivariate allometric equation was used as Y = aXb. The scatterplot was discontinuous, presenting two trends during the postsomitic period. The first part was composed of embryos staged from stages 15 to 20, and the second part by embryos staged from stages 21 to 23. The breakpoint between these different trends was found at the level of stage 20 (embryo of 22 mm in crown-rump length and age nearly of 52 days). From stages 15 to 20, the growth rate of the myocardium was allometrically negative. On the other hand, from stages 21 to 23 this growth rate was moderately allometrically positive. These differences in growth of the myocardium were analyzed and, at least partially, might be due to the functional circulatory increase in the peripheral vascular bed in correlation to the cardiac hemodynamic demand required at the end of the embryonic period prope
ISSN:1422-6405
DOI:10.1159/000147130
出版商:S. Karger AG
年代:1991
数据来源: Karger
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