摘要:

Gastrin-secreting G cells and enterochromaffin cells have been examined in the duodenum of the gerbil-Meriones unguiculatus. G cells examined by immunoperoxidase and immunofluorescence methods were seen primarily located in the mucosa lining the villi and rarely in the glands of Lieberkühn. They were flask-shaped cells that extended from the basal lamina to the mucosal surface. Immunoreactive gastrin was localized all over in the cytoplasm but was especially concentrated in the juxtanuclear and subnuclear regions. Enterochromaffin cells were identified by a serotonin-specific formaldehyde-induced fluorescence method. These cells were also primarily located in the duodenal villi, were flask-shaped and revealed an intense yellow fluorescence that was especially concentrated in the subnuclear cytoplasm. At the ultrastructural level the enterochromaffin cells showed numerous pleomorphic electron-dense secretory granules especially located in the subnuclear cytoplasm. Microfilament bundles were often observed, especially in the perinuclear region

ISSN:1422-6405    

DOI:10.1159/000145501

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Guinea pig thyroid tissue was incubated in a tissue culture system in the presence of various TSH concentrations and for various time periods. The morphokinetic response was examined with the electron microscope. The qualitatively finer aspects of the TSH-caused sequence of events are described and compared with in vivo results. After in vitro stimulation the general changes of cell membranes and of the cytocavitary network are very similar to those in vivo. On the other hand, there are differences with regard to intensity and duration of the structural response to TSH and additionally degenerative changes of organelles are found which might be explained by incubational factors. The role of the mitochondria as a source of energy is demonstrated by typical qualitative and quantitative alterations. An interesting finding is the degradation of mitochondria within colloid droplets, a mechanism which is suggested to represent a modified autophagy. Intracellular follicles are occasionally found during in vitro experiments. The formal genesis of this structure and its possible role is discussed. The present study indicates that this incubation model provides a useful tool for further analyses of thyroid disorders.

ISSN:1422-6405    

DOI:10.1159/000145502

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The primary purpose of this study is to present a description of the morphology of the middle ear in the rhesus monkey (Macaca mulatta) based on original dissections. The results presented document the morphologic similarities of this structure in the rhesus monkey and man, and further define the anatomic parameters necessary for using the rhesus monkey to model the human system.

ISSN:1422-6405    

DOI:10.1159/000145503

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Increase in the amount of secretary material (interreceptor matrix) surrounding the visual cells was observed after laser treatment. At the same time, an increase in labelled uridine uptake by the pigment epithelium was detected. The increase uptake was highest 4 h after laser treatment.

ISSN:1422-6405    

DOI:10.1159/000145504

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The outer capsule of rat superior cervical ganglia is usually removed before in vitro studies are initiated. An ultrastructural study was undertaken to determine if any morphological disruption results as a consequence. Intact ganglia were fixed by either aldehyde immersion or perfusion. Desheathed ganglia were immersed in aldehyde fixative immediately following the removal of the capsule or after a 5-hour incubation period in a modified Krebs-Ringer solution. Ganglionic transmission, monitored by observing the compound postganglionic action potential evoked by periodic preganglionic nerve stimulation during the incubation period, was not diminished. Some minor disruption of mitochondrial cristae was observed in neurons but ganglionic morphology was generally well preserved. These findings support the use of desheathed rat ganglia as a valid in vitro model.

ISSN:1422-6405    

DOI:10.1159/000145505

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The distributions of β-galactosidase and β-glucuronidase in the forebrain of the hedgehog are quite similar to the distribution of phospholipids. Both enzymes and the phospholipids are mainly localized in the fibrous components. Their pattern of distribution is also identical to myelin staining. The functional significance of enzymes in lipid and myelin metabolism is discusse

ISSN:1422-6405    

DOI:10.1159/000145506

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Histological, histochemical fluorescence, and electron microscopic techniques have been utilized to demonstrate the specific distribution of the noradrenergic sympathetic postganglionic fibers to the guinea pig left atrium and ventricle. The majority of these nerves entered the base of the heart. Paravascular bundles of unmyelinated sympathetic postganglionic fibers traversed the left atrioventricular sulcus. From these paravascular plexuses axons accompanied the arteries via periarterial plexuses to the deeper layers of the heart wall. The vascular tree was extremely complex. In the left atrium a relatively abundant noradrenergic innervation was seen within the interstitial connective tissue surrounding the cardiac muscle. In the left ventricle, most of the noradrenergic innervation was located in the tunica adventitia of all the major coronary arterial branches before they entered the cardiac muscle. These larger branches of the coronary arteries were predominantly innervated by the sympathetic postganglionic fibers. Apparently it is from these perivascular nerve plexuses that the left ventricular muscle receives the majority of its noradrenergic innervation.

ISSN:1422-6405    

DOI:10.1159/000145507

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The vomeronasal neuroepithelium of the vampire bat, Desmodus rotundus, and the frugivorous bats, Carollia perspicillata and Artibeus jamaicensis, was examined at the light-microscopic and ultrastructural levels. The neuroepithelium of these species is basically similar in morphology to that of other mammals. However, hitherto unknown differences were noted in regard to the configuration of the apical poles of cells, in the occurrence of solitary cilia, dislocated sensory cells, brush cells, cell contacts and the occurrence and fine structure of basal cells. The morphology of the vomeronasal neuroepithelium of all three species is discussed from a comparative point of view in regard to chemoreception.

ISSN:1422-6405    

DOI:10.1159/000145508

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The structure of the human ovarian ligament was studied with light and electron microscopy, with emphasis on neural components. The ligament is primarily composed of smooth muscle arranged in an outer oblique and an inner longitudinal layer. The smooth muscle cells are connected by two types of intercellular junctional complexes, close contacts and intermediate junctions. The intercellular junctions may serve to coordinate smooth muscle activity and synchronize contractions of the ligament. Nerves are abundant and accompany the vascular supply. Specialized encapsulated neural structures previously reported were not observed. Acetylcholinesterase-positive nerves are more numerous than adrenergic nerves. Both types of nerves are associated with vascular smooth muscle. We conclude that these nerves either traverse the ligament or terminate in the ligament and are vasomotor.

ISSN:1422-6405    

DOI:10.1159/000145509

出版商:S. Karger AG    

年代:1982

数据来源: Karger