ISSN:0098-1532    

DOI:10.1002/mpo.2950020302

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1976

数据来源: WILEY

ISSN:0098-1532    

DOI:10.1002/mpo.2950020303

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1976

数据来源: WILEY

摘要:

AbstractDNA‐dependent RNA polymerase II has been purified from lymphocytes of patients with chronic lymphocytic leukemia (CLL). Form II polymerase and Escherichia coli RNA polymerase have been used to study the transcription of human DNA and chromatin. The hybridization kinetics of the transcripts of DNA and chromatin by the human polymerase are quite different; chromatin transcripts hybridized to DNA at a much slower rate than DNA transcripts, whereas the transcripts of DNA and chromatin by bacterial polymerase have similar hybridization kinetic

ISSN:0098-1532    

DOI:10.1002/mpo.2950020304

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1976

数据来源: WILEY

摘要:

AbstractMonolayer cultures of Chinese hamster ovary (CHO) cells take up the photo‐decomposition products of DIC more readily than DIC itself. Dimethylamine, an immediate product of this degradative pathway, can ultimately become associated with the DNA, RNA, and protein of the cells as demonstrated by selective enzymatic degradation of macromolecules and isopycnic centrifugation. The relevance of these observations to mechanism studies of DIC is discusse

ISSN:0098-1532    

DOI:10.1002/mpo.2950020305

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1976

数据来源: WILEY

摘要:

AbstractRecent studies of the structure of messenger RNA have demonstrated the existence of untranslated sequences of the 3′ and 5′ end of the messages. In addition analysis of transcription in vitro has indicated that the nucleotide sequence U6 purine may be part of a transcription termination signal in prokaryotes. Recently it has been possible to determine the sequence of extensive portions of the DNA of SV40 virus. This article reviews the analogies between certain of these sequences and sequences available from prokaryotic messengers and DNAs. Unusual structures, including blocks of AT‐rich and GC‐rich segment sections and symmetric regions in the DNA near the origin of DNA replication, have been demonstrated and the distribution of stretches of 6 or more deoxyadenylic acids in the DNA of SV40 is consistent with some rho for these sequences in animal cells, either as terminators of transcription or as sites where degradation of transcripts is initiated or sites related to the selective rejection or degradation of tran

ISSN:0098-1532    

DOI:10.1002/mpo.2950020306

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1976

数据来源: WILEY

摘要:

Abstract5‐Azacytidine 5′‐triphosphate (5‐aza‐CTP) inhibited the phosphorylation of uridine, cytidine, and 5‐azacytidine (5‐aza‐C) in a reaction catalyzed by uridine‐cytidine kinase. The inhibition appeared to be competitive with respect to the adenosine 5′‐triphosphate and noncompetitive with respect to nucleoside substrates. 5‐aza‐CTP was a potent inhibitor of 5‐aza‐C phosphorylation but a weak inhibitor of uridine and cytidine phosphorylation. These results suggest that the feedback inhibition of uridine‐cytidine kinase by 5‐aza‐CTP may limit the amount of intracellular nucleotide

ISSN:0098-1532    

DOI:10.1002/mpo.2950020307

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1976

数据来源: WILEY

摘要:

AbstractA hamster fetal cell clone has been developed for in vitro chemotherapeutic studies as well as in an in vivo fibrosarcoma model system. Highly reproducible quantitative in vitro chemotherapeutic data can be obtained with this cell line within 5 days, and as few as 102cells produce rapidly growing fibrosarcomas when injected subcutaneously into adult hamsters. We found using these cells in vitro that 1‐β‐D‐arabinofuranosylcytosine (ara‐C) can antagonize the effect of 5‐azacytidine (aza‐C) if given simultaneously or if aza‐C treatment is preceded by a 2‐hr exposure to ara‐c. Using the same cell line as in vivo model for chemotherapy it was also shown that ara‐C and cyclocytidine significantly inhibited tumor growth. This hamster cell line may be quite useful as an in vitro/in vivo model system for the study of cancer che

ISSN:0098-1532    

DOI:10.1002/mpo.2950020308

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1976

数据来源: WILEY

摘要:

AbstractThe C1300 murine neuroblastoma system has been studied to determine its relevance as a chemotherapy model to the human disease. Studies using combination therapy revealed that BCNU/cyclophosphamide combination therapy increased the median lifespan 300% in A/J mice bearing the C1300 tumor. Cyclophosphamide/imidazole carboxamide and adriamycin/imidazole carboxamide combinations were less active, increasing median lifespans 189% and 144%, respectively. Vincristine/bleomycin were inactive even when the schedule was adjusted to coincide with the time of maximum mitoses following vincristine injections. These results suggest the BCNU/cyclophosphamide combination chemotherapy may be effective in human disease.

ISSN:0098-1532    

DOI:10.1002/mpo.2950020309

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1976

数据来源: WILEY

摘要:

AbstractThe cytotoxicity of many antitumor agents exhibits cell‐cycle phase specificity. Using a human lymphoma cell line synchronized by thymidine block, we have investigated the differential uptake of radioactive 1‐β‐D‐arabinofuranosylcytosine (ara‐C) and prednisolone in various phases of the cycle. Uptake of ara‐C is highest during early S and declines steadily throughout the rest of the cycle. In comparison, prednisolone demonstrates no significant age‐dependent difference in uptake although its cytotoxicity is cell c

ISSN:0098-1532    

DOI:10.1002/mpo.2950020310

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1976

数据来源: WILEY

摘要:

AbstractThree courses of intensive consolidation therapy were administered to 13 patients with acute leukemia after they achieved complete remission. The patients were randomly allocated to receive their therapy in or out of a protected environment unit and with or without antibiotic prophylaxis. The results suggest that the protected‐environment—prophylactic‐antibiotic program may have protected the patients from infection, although the numbers in each group are too small for meaningful comparison. However, early consolidation therapy was not beneficial, since the duration of remission and survival of these patients was less than that of a group of comparable patients who received only conventional maintenance th

ISSN:0098-1532    

DOI:10.1002/mpo.2950020311

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1976

数据来源: WILEY