摘要:

A simplified radioimmunoassay is described for routine determination of testosterone in plasma, using antiserum to a testosterone‐3‐conjugate, and3H‐testosterone as the sole radio‐ligand. The method avoids chromatography by using the plasma sex‐steroid binding globulins to effect a partial purification. The radioimmunological method is compared with a specific, chromatographic, competitive protein binding method. Testosterone concentrations in normal subjects are 7.84±2.38 (SD) ng/ml in men, and 0.81±0.17 (SD) ng/

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1974.tb02810.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Plasma aldosterone, plasma cortisol, plasma renin activity and the serum concentrations of sodium and potassium were determined at short time‐intervals in four supine anephric patients. Two patients were studied the night before and the night following haemodialysis. In both patients increases in plasma aldosterone occurred without concomitant alterations in serum sodium and serum potassium while plasma renin activity was uniformly undetectable. However, the observed fluctuations in plasma aldosterone were markedly more pronounced the night following haemodialysis. Under the latter conditions, typical secretory episodes of aldosterone occurred. The onset of these secretory episodes was not associated with simultaneous increases in plasma cortisol, whereas before haemodialysis changes in plasma aldosterone were paralleled by those in plasma cortisol. Except for the peak values of the secretory episodes of aldosterone, plasma aldosterone was markedly higher the night before than the night following haemodialysis. These differences between pre‐ and post‐haemodialysis aldosterone values correlated well with serum potassium, which was approximately 1 mEq/1 lower the night following haemodialysis. Two patients were studied the night following haemodialysis under normal conditions and with suppression of ACTH secretion by dexamethasone. Under both conditions, a typical episodic secretion of aldosterone was observed. In both patients plasma ACTH was below the lower limit of detectability (<20 pg/ml) under dexamethasone medication. It was concluded from our experiments that: (1) the differences between pre‐ and post‐haemodialysis aldosterone values reflect a direct influence of potassium on the secretion of aldosterone; (2) the fluctuations in plasma aldosterone which were observed the night before haemodialysis were mostly probably mediated through ACTH; and (3) an unknown factor had caused episodic secretion of aldosterone the night following haem

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1974.tb02811.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

The disappearance of LH‐releasing hormone (LH‐RH) administered i.v. together with inulin was measured by radioimmunoassay. The half‐time disappearance of 3.6 min for LH‐RH was significantly shorter than the half‐time of 14.2 min found for the inulin. On the assumption that diffusion and distribution into the extracellular space and excretion are quantitatively similar for LH‐RH and inulin, the part of the half‐life of LH‐RH due to metabolic breakdown can be calculated to be about 4.8 min. Peak LH and FSH values were found to occur later. The results suggest that most of the immunoreactive LH‐RH is no longer present in the blood at the time of maximal gonadotropin release and that this is because of rapid metabolism as well as diffus

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1974.tb02812.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

A combined test with LHRH and TRH was investigated in the normal female subject during the menstrual cycle. LH and FSH responses were not affected by raised prolactin or TSH levels after TRH.No correlation was seen between either basal levels or responses of prolactin and TSH after TRH, and no difference in responses on days 4 or 24 were observed. The increments in prolactin and TSH were significantly greater in female than in male subjects.Although FSH responses to LHRH+TRH were not significantly different, LH responses on day 24 were greater than on day 4. A significant linear correlation between FSH and LH responses to LHRH was seen.The results indicate that prolactin and TSH responses to TRH are greater in female than male subjects and that changes in LH and FSH after LHRH do affect these responses. Normal ranges for hormone responses after LHRH and TRH are defined.

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1974.tb02813.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Serum levels of LH‐RH, LH and FSH were measured by radioimmunoassay at frequent intervals in four young men following the intranasal administration of 2 mg synthetic LH‐RH. The initial rise in serum LH‐RH was seen at 2.5 min and the peak was reached at 15 min. There was a rapid response in serum LH, the peak occurring between 30 and 45 min, while FSH levels did not change significantly. It was calculated that 1.25% of the dose was absorbed. The intranasal administration of LH‐RH appears to have considerable therapeutic po

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1974.tb02814.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

In normal man, synthetic linear somatostatin (growth hormone‐release inhibiting hormone) inhibits the growth hormone response to insulin induced hypoglycaemia, but has no influence on plasma levels of cortisol, prolactin, TSH and FS

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1974.tb02815.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

An unusual case of testicular feminization is reported, characterized by a 47 XYY karyotype in blood and gonads. Plasma testosterone varied between 509 and 557 ng/100 ml. Testosterone production was normal at 6.18 mg/day. There was very little response to human chorionic gonadotrophin stimulation. Gonadectomy revealed small testes, only one of which was shown to be metabolically active.

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1974.tb02816.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Urinary testosterone levels were measured in twenty‐eight hirsute women and ten control subjects before and after the administration of corticotrophin. Twenty‐four patients had elevated basal urinary testosterone levels, and of these, nineteen showed an abnormal response to corticotrophin stimulation. Ten hirsute women from the latter group of nineteen, and eight control subjects, were further studied by measurement of the urinary pregnanetriol excretion before and following the concurrent administration of corticotrophin and metyrapone. An abnormal increment in pregnanetriol excretion was observed in eight out of the ten hirsute patients. It is suggested that this is evidence that an adrenal abnormality may be responsible for certain cases of idiopathic hirsutism, which may be operative at the 21‐hydroxylase

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1974.tb02817.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

3H‐oestradiol was injected subcutaneously into patients 2 hr prior to hysterectomy. The amount of labelled hormone present at operation in fractions of uterine endometrium and in other tissues was measured.Patients pretreated with tamoxifen (I.C.I. 46,474) had lower levels of3H‐oestradiol in the nuclei of the uterine endometrium and of total labelled material in the myometrium than did untreated patients.In vitro, tamoxifen also inhibited the uptake of3H‐oestradiol into the nuclei of human uterine endometrium during tissue incubations and its binding to the cytosol receptors.Fibroids also showed active oestradiol uptakein

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1974.tb02818.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

A specific, accurate, precise and simple radioimmunoassay for thyroxine (T4) in small volumes of unextracted serum is described. It discriminates well between levels found in different states of thyroid function. Levels in euthyroid subjects range between 4.3 and 11.2 μg/100 ml (mean 7.7); in untreated hyperthyroid subjects, 13.2‐25.6 μg/100 ml (mean 19.2); and in untreated hypothyroid subjects undetectable to 2.6 μg/100 ml (mean 1.4). There is satisfactory correlation with competitive protein binding and protein bound iodine methods. Because of its advantages over previous techniques, it is suggested that T4radioimmunoassay will become the routine method of assessing thyroid status.The measurement of serum thyroxine (T4) is the single most important test in the routine laboratory assessment of thyroid status. Ideally the T4assay should be specific, cheap, simple and have a high sample capacity. Thyroxine is determined most commonly in serum extracts by competitive protein binding (CPB) methods (Murphy&Pattee, 1964; Ekinset al., 1969) or, indirectly, by estimation of serum protein bound iodine (PBI). Both techniques require the processing of relatively large volumes of serum before assay. The PBI method has the added disadvantage of measuring iodine‐containing compounds other than thyroxine (Acland, 1971).We report here a specific, precise and simple radioimmunoassay for thyroxine in unextracted human serum and its application to the assessment of thyroid status. It has significant advantages over CPB, PBI and previous radioimmunoassay m

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1974.tb02819.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY