摘要:

SUMMARYA new kit for measuring total serum triiodothyronine (T3) by radioimmunoassay (‘T3 RIA’; Radiochemical Centre, Amersham) was evaluated using sera from 1114 patients and normal controls. The kit performed reliably with intraassay and inter‐assay variability figures of 3.9% and 9.3%, respectively, at‘medium’concentrations of T3. A T3 measurement could be obtained conveniently within 18 h after overnight incubation at room temperature. There were no critical steps dependent on time or temperature.Serum T3 values showed no significant sex difference. There was no significant change in mean serum T3 between the ages of 15 and 69 years, but it fell by 0.15 nmol/1 for every 5 years beyond the age of 70. Mean serum T3 (±SD) for 335 normal euthyroid subjects aged 15 ‐69 years was 2.11 ±0.46 nmol/1 (range: mean±2 SD= 1.19‐3.03 nmol/1).64% of pregnant euthyroid women and 12% of those taking oral contraceptives had elevated serum T3 levels, as did all hyperthyroid patients, apart from one with T4 toxicosis. Overlapping T3 values from hyperthyroid patients and euthyroid subjects with elevated concentrations of thyroid binding proteins could be separated completely by two correction techniques which related total serum T3 to the corresponding T3 resin uptake test, viz an‘augmented free T3 index, or a map plot of T3 vs T3 resin uptake. A 25% incidence of T3 toxicosis was observed. One hyperthyroid patient with T4 toxicosis, and seven euthyroid patients with‘biochemical T4 toxicosis’were investigated. 73% of moderately and 30% of severely hypothyroid patients had normal serum T3 levels. This overlap was not reduced by applying correction techniques.Our studies demonstrate the value of serum T3 measurements in screening for and diagnosing hyperthyroidism. As T3 measurements become more widely available, it would seem reasonable to subclassify hyperthyroid patients into three types: those with‘T3/4 toxicosis, T3 to

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1977.tb03313.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

SUMMARY1. Results of tests for the diagnosis of Cushing's syndrome of various aetiologies are discussed for twenty‐five patients in whom the pathology was established by operation or autopsy.2. Control values for the urinary excretion of free Cortisol, 17‐OHCS, Porter‐Silber chromogens (P‐SC) and 17‐OS and plasma levels of P‐SC are compared with those for normal subjects.3. The results indicated that urinary values are within the normal range for some patients with Cushing's syndrome.4. Plasma levels of P‐SC in the morning were within the normal range for the majority and elevated for the rest.5. Some patients showed day‐night variation of plasma P‐SC but evening values were above the normal range.6. The expected response for low dosage dexamethasone was found in all patients tested but unexpected responses followed high dosage in some.7. Plasma 11‐OHCS in the five patients tested failed to respond to insulin induced hypoglycaemia.8. Metyrapone administration and corticotrophin infusion tests had limited usefulness in establishing the aetiology of the disease. The 17‐OHCS excretion became raised in the response to corticotrophin and the elevation was prolonged beyond

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1977.tb03314.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

SUMMARYA familial type I hyperlipoproteinaemia is described in three members of a family of eleven; on the basis of LPL activity and HDL content of plasma three other members of the family have been diagnosed to be heterozygotes without other disturbances in their lipid spectrum. The distribution of this lipid disorder is in accordance with an autosomal recessive inheritance pattern. In this family a second hereditary condition, thyroxine binding globulin deficiency, was found in addition to the hyperlipoproteinaemia. The inheritance of this condition appears to be as an autosomal dominant. An interrelated inheritance pattern of both conditions could not be proved, but both traits may be located on the same chromosome at some distance from another to allow recombination.

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1977.tb03315.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

SUMMARYThyroid‐stimulating immunoglobulins (TSI) have been detected by receptor assay in the sera of 43% of patients with ophthalmic Graves’disease. Comparison of the receptor assay studies with thyroid function tests indicated that in several patients the antibodies detected by receptor assay were biologically inactive. In other patients, thyroid function appeared to be under TSI control with hyperthyroidism prevented by autoimmune destruction of the thyr

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1977.tb03316.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

SUMMARYTen endocrinologically normal men with secondary sexual impotence were given 500 μg LHRH subcutaneously every 8 h. After 4 weeks treatment the LH response to 500 μg LHRH was reduced from a peak of 35.7 ± 5.2 to 16.8 ± 3.5 mu/ml(P<0.01) and the FSH response from 4.2 ± 0.93 to 2.39 ± 0.4 mu/ml(P<0.01). Circulating total testosterone, oestradiol, prolactin and sex hormone binding globulin showed no significant changes. Whether this inability of the pituitary to maintain its response to LHRH is peculiar to impotent men requires further

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1977.tb03317.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

SUMMARYSerum melatonin was measured by radioimmunoassay at 4‐hourly intervals for 24 h in five normal men, and shown to exhibit nyctohemeral variations. The highest concentrations of serum melatonin occurred at 02.00 hours and the lowest concentrations at 14.00 hours. The synthetic enzymes for melatonin, serotonin‐N‐acetyl transferase (SNAT) and hydroxyindole‐O‐methyltransferase (HIOMT) were measured in post‐mortem pineal organs from thirty‐six men and women, and the activity of both enzymes was directly related to the time of death of the subject, resulting in a similar nyctohemeral curve as that observed with serum melatonin. The SNAT and HIOMT enzymes were stable when measured in pineal organs held under the usual post‐mortem conditions.These observations indicate a nyctohemeral rhythm in human pineal function and suggests that any clinical interpretation of pineal dysfunction in disease states should take into consideration thi

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1977.tb03318.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

SUMMARYThyroid hormone affects both developmental and metabolic processes. It has a relatively specific effect on the synthesis of a number of enzymes and other proteins. The fundamental cellular mechanism of action seems to be at the level of genetic regulation. It involves interaction with nuclear receptors, leading to an activation of the protein synthesizing machinery. How binding to receptors is coupled to genetic activation is completely unknown. At least part of the metabolic effects of thyroid hormone could be mediated through an interaction with mitochondria and cell membrane, and with some enzymatic systems such as adenylcyclase.

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1977.tb03319.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Book review in this ArticlesMolecular Endocrinology of the Steroid Hormones. By D. SCHULSTER, S. BURSTEIN and B. A. COOKE.The Endocrine Function of the Human Ovary. Edited by V. H. T. JAMES, M. SERIO and G. GIUSTI. Academic Press Inc. (London) Ltd.Hormone Assays and their Clinical Application, 4th edn. Edited by JOHN A. LORAINE and E. TREVOR BELL. Churchill Livingstone. EdinburghDiseases of the Thyroid. By D. C. EVERED.

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1977.tb03320.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

ISSN:0300-0664    

DOI:10.1111/j.1365-2265.1977.tb03321.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY