ISSN:0014-3022    

DOI:10.1159/000115221

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

By analysis of a kinetic model of the behavior of 2-deoxyglucose and glucose in brain, an operational equation has been derived that allows the computation of the rates of glucose utilization simultaneously in all structures of the central nervous system on the basis of readily measurable variables. Localization in the central nervous system is achieved by a quantitative autoradiographic technique that provides direct visualization of the relative rates of glucose utilization in all parts of the central nervous system.

ISSN:0014-3022    

DOI:10.1159/000115222

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

In case of quick physiological variations or in pathological conditions, it is impossible to measure the deoxyglucose consumption by means of the deoxyglucose technique and we propose a method for the study of the deoxyglucose uptake by the cerebral tissue. It is simultaneously measured with the blood flow, 3 min following the intravenous administration of deoxyglucose (2-deoxyglucose-14C). Hypercapnia induces an increase in the flow but does not modify the deoxyglucose uptake and moderate hypoxia results in a decrease in the deoxyglucose uptake without modifying the flow. In pathological conditions such as ischemia or stricture, the blood flow and deoxyglucose uptake variations are not parallel. These results show that the 2-deoxyglucose uptake does not follow the blood flow and that it depends on the cell activity. It is therefore possible to use this method when the consumption studies cannot be implemented.

ISSN:0014-3022    

DOI:10.1159/000115223

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

The problems of extending the deoxyglucose method for insects to electron microscopical resolution are discussed. It is calculated that several curies of 3H-deoxyglucose per kilogram body weight may be needed for identification of activity in ultra-thin sections. Oral application may be superior to injection. Incorporation of experimental and control stimulus in the same animal seems appropriate. Freeze-drying may turn out to be the best method of preparing the tissue for plastic embedding. The problem of cutting on a water surface is discussed. It is demonstrated that freeze-drying of Drosophila results in structural preservation sufficient for cell identification in the cervical connective. Autoradiography of ultra-thin sections has not yet been carried out.

ISSN:0014-3022    

DOI:10.1159/000115224

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

Light and high resolution radioautography were performed on cat and guinea pig inner ear after an in vivo administration of tritiated deoxyglucose and conventional histological treatments. In the guinea pig cochlea a diffuse radioautographic reaction product appeared on all the neurosensory and surrounding structures, with a more intense labelling of stria vascularis and Reissner’s membrane. In the cat vestibular organ a diffuse reaction was also noted, with an intense clear-cut labelling of some dark cells at the base of the lateral crista and on some cells limiting the endolymphatic space, opposite the utricular macula. At the EM level, the silver grain distribution preferentially appeared to be localized on the cytoplasmic glycogen granules. In addition, some sensory cells were densely marked, the silver grains neither occurring on the nucleus nor on the surrounding nerve calyx, and rarely upon the mitochondria. These data indicate that using classical histological treatments, it is possible to retain a part of the radioactive molecules around the injection site. The possible physiological meaning of the selective labelling of some cellular and subcellular compartments is discusse

ISSN:0014-3022    

DOI:10.1159/000115225

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

Local cerebral glucose utilization (LCGU) was determined in 2- to 8-day-old beagle dogs by the quantitative 14C-deoxyglucose autoradiographic method. In physiologically controlled puppies (BP 66 ± 3 mm Hg; paO2 71 ± 2 mm Hg; paCO2 35 mm Hg, pH 7.39 ± 0.04; hematocrit 39 ± 2%), the 2-deoxyglucose (2-DG) autoradiographs and quantitative data reveal a characteristic pattern of regional energy metabolic needs in the normal newborn brain: highest glucose consumption values are found in brain stem nuclei (inferior olivary nucleus 19 ± 4 µmol/100g/min; vestibular nucleus 26 ± 5; red nucleus 15 ± 2) and in selected deep cerebral structures (subthalamic nucleus 18 ± 4; ventrolateral thalamic nucleus 12 ± 2,...) whereas consistently relative lower glucose consumption is found in the cerebral cortex (mean 7 ± 1). This data is in agreement with local cerebral blood flow studies in the newborn puppy. A characteristic functional anatomy of the newborn dog brain is demonstrated. The value of 0.558 ± 0.031 for the lumped constant was used in the calculation of LCGU. In puppies previously asphyxiated for 2.5–3.5 min 1–2 days prior to the 2-DG procedure, no significant effect upon regional metabolism w

ISSN:0014-3022    

DOI:10.1159/000115226

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

The 2-deoxy-D-[14C]-glucose method for the quantitative determination of local cerebral glucose utilization has been applied to the mouse. The experiments were conducted in awake freely moving animals. Local cerebral glucose utilization was found to vary widely throughout the brain with the lowest values in white matter and the highest in gray matter. The most metabolically active structures were those involved in auditory function or in general motor activity. All the values were lower than those observed previously in the conscious rat. The use of free-moving animals may be of importance since it allows the study of physiological or pathological behavioral states.

ISSN:0014-3022    

DOI:10.1159/000115227

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

Using the autoradiographic deoxyglucose method, local consumption of glucose has been visualized during convulsive seizures induced by systemic administration of kainic acid, bicuculline or metrazol. A striking correlation was found between the time course of the electrographic and metabolic alterations in limbic structures following kainic acid administration. In addition, pathological alterations were almost exclusively observed in structures in which there had been a rise in metabolism. In contrast, following bicuculline or metrazol a rise in metabolism was conspicuous primarily in the cerebellum, vestibular nuclei and neocortex.

ISSN:0014-3022    

DOI:10.1159/000115228

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

Using the quantitative 14C-2-deoxyglucose method to measure glucose utilisation in discrete areas of the brain, we have shown that chloral hydrate anaesthesia causes a marked depression of cerebral metabolism in all grey matter areas measured. Moreover, chloral hydrate anaesthesia can reduce, abolish or reverse the action of apomorphine upon glucose utilisation from that seen in conscious animals.

ISSN:0014-3022    

DOI:10.1159/000115229

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

The 2-deoxyglucose uptake method studied by autoradiography was used to determine the modification produced by a thirst-dependent state. The density of the subfornical organ, the suprachiasmatic area and the nucleus medianus was higher in dehydrated than in normohydrated animals. These areas are certainly involved in the detection of dehydration and in thirst.

ISSN:0014-3022    

DOI:10.1159/000115230

出版商:S. Karger AG    

年代:1981

数据来源: Karger