摘要:

The number of ribosomal DNA (rDNA)-containing chromosomes and the amount of total rDNA vary widely among primate species. In spite of numerical variations, the mean number of ribosomal (rRNA) genes that participate in nucleolar organization is approximately the same.

ISSN:1424-8581    

DOI:10.1159/000132121

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

The high-resolution RBA-banding pattern of bovine chromosomes (Bos taurus L.), together with a diagrammatic representation, are presented. The haploid set of prometaphase chromosomes shows 521 bands, 183 of which are positive, 257 negative, and 81 variable. Compared to early metaphase chromosomes, the prometaphase chromosomes show 50% more bands, resulting in a more accurate description of the banding pattern of individual chromosomes of this species.

ISSN:1424-8581    

DOI:10.1159/000132122

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

We have used in situ hybridization techniques to determine the subchromosomal location of the mouse cKi-ras protooncogene. The data confirm the assignment of cKi-ras to mouse chromosome 6 and provide evidence that the gene maps to the distal portion of this chromosome, in bands 6F3→G

ISSN:1424-8581    

DOI:10.1159/000132123

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Muntjac prophase and metaphase chromosomes were G-banded following methotrexate-mediated synchronization of peripheral lymphocytes. Bands and subbands were characterized from prophase through metaphase, and the progression of band patterns from late prophase to mid-metaphase was analyzed. Extended prophase chromosomes exhibited more bands and subbands, a number of which became fused with each other, giving rise to fewer and thicker bands in the condensed metaphase chromosomes. It appeared that the dark bands condensed relatively more than the light bands. Precise delineation of the bands and subbands on extended prophase chromosomes and the usage of a proposed banding pattern nomenclature should aid in better detection and localization of induced chromosomal rearrangements with this extremely useful experimental material.

ISSN:1424-8581    

DOI:10.1159/000132124

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Human chromosomes were separated by a dual laser FACS sorter and their DNA hybridized with a thyroglobulin gene probe. A strong hybridization signal was obtained with DNA from chromosome 8. A panel of mouse-rat cell hybrids was used to determine the chromosomal localization of the rat thyroglobulin gene by the Southern blotting method. Comparison of the cytogenetic data with the hybridization signals obtained with the rat thyroglobulin probe allowed assignment of this gene to rat chromosome 7. It is concluded that the synteny relationship between the thyroglobulin gene and the c-myc oncogene has been conserved in rat and man.

ISSN:1424-8581    

DOI:10.1159/000132125

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Cells of the Chinese hamster line EM9 exhibit an elevated SCE frequency and a decreased proliferative response in bromodeoxyuridine (BrdU)-containing medium. Here, these phenotypic features were examined experimentally using polyethylene glycol fusion of EM9 and normal human cells. EM9/human hybrids exhibited both normal SCE frequencies and normal proliferative responses in BrdU-containing medium, suggesting that SCE elevation and BrdU hypersensitivity in EM9 have a common molecular basis.

ISSN:1424-8581    

DOI:10.1159/000132126

出版商:S. Karger AG    

年代:1985

数据来源: Karger

ISSN:1424-8581    

DOI:10.1159/000132127

出版商:S. Karger AG    

年代:1985

数据来源: Karger