摘要:

Cytogenetic analyses of bone marrow and gonadal cells in a male mouse, which appeared to be normal, revealed mosaicism in both tissues. Three chromosome complements, 39, X, 40, XY, and 41, XYY, were found in both bone marrow and spermatogonia, while only the last two complements were found in spermatocytes. In this mouse, unlike in the human, the XYY cells showed a proliferative advantage over the XY cells. In XYY cells at diakinesis/metaphase I the gonosomes showed all possible types of association, and a pairing advantage of the X chromosome was clearly demonstrated. The fertility of the mouse was not determined. However, since the epididymal sperm count was reduced by only 55% and the incidence of sperm head abnormality was near normal, it is not evident that the mouse was sterile.

ISSN:1424-8581    

DOI:10.1159/000132046

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

The methods of somatic cell genetics and molecular hybridization were applied to a panel of mouse × rat hepatocyte hybrids segregating rat chromosomes to assign the rat genes coding for two serum proteins, albumin and α-fetoprotein (Alb and Afp). The molecular hybridization of DNAs from different hybrids with cloned DNA probes showed that all the hybrid clones possessing the rat Alb gene and expressing it also retained the rat Afp locus, which is not expressed in these hybrids. So the Alb and Afp genes are syntenic in the rat, as in the mouse. Furthermore, the cytogenetic analysis allowed the assignment of these two loci to rat chromosome 1

ISSN:1424-8581    

DOI:10.1159/000132047

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

The structural gene coding for human arylsulfatase B, ARSB, is assigned to 5p11→5qter by analysis of somatic cell hybrids isolated from two separate fusions of human fibroblasts carrying a translocation involving chromosome 5 with the Chinese hamster cell line a

ISSN:1424-8581    

DOI:10.1159/000132048

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

Retinoblastoma (Rb) cells may be more sensitive to X-irradiation than normal cells when assayed for clonogenic survival, suggesting that such sensitivity can be used as a trait for detecting RBI gene carriers. We studied induction of chromosome damage in lymphocytes from patients with Rb by the radiomimetic antineoplastic antibiotic, bleomycin (BL). Lymphocytes were treated in G2 for 4 h at four concentrations of the drug (0.0, 0.3, 0.9, and 2.7 µg/ml), and chromosome damage was evaluated. No difference in levels of spontaneous or induced damage was detected among hereditary Rb, non-hereditary Rb, and normal lymphocytes. The role of X-ray treatment in the induction of second tumors in Rb patients is discussed

ISSN:1424-8581    

DOI:10.1159/000132049

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

Human haptoglobin (Hp) is a protein that binds free hemoglobin and circulates in plasma of vertebrates as a tetrachain (αβ)2 structure. This study maps HPA and HPB, the genes encoding the Hp α and β chains to human chromosome band 16q22 by in situ hybridizat

ISSN:1424-8581    

DOI:10.1159/000132050

出版商:S. Karger AG    

年代:1984

数据来源: Karger

ISSN:1424-8581    

DOI:10.1159/000132051

出版商:S. Karger AG    

年代:1984

数据来源: Karger

ISSN:1424-8581    

DOI:10.1159/000132052

出版商:S. Karger AG    

年代:1984

数据来源: Karger