摘要:

First cleavage divisions were analyzed, after chromosome banding, in 321 zygotes recovered from superovulated, outbred ICR mice 33–35 h after injection of human chorionic gonadotropin. With 72.1% of all zygotes at metaphase and late prophase analyzed, there was 87% diploidy, 3.6% trisomy, 2.5% monosomy, 0.4% structural rearrangement, and 0.93% triploidy. Aneuploid zygotes in which there were 37 or 38 chromosomes accounted for 6.5% of the population with both haploid complements analyzable. In 45 diploid zygotes in which only one complement was analyzable, there was 93.4% haploidy, 2.2% disomy, and 4.4% hypoploidy. The chromosome most often involved in aneuploidy was the 19. The parental origin of the chromosome anomalies was determined on the basis of differential condensation of the chromosomes. The source of trisomy was shown to be the male in three and the female in five of the eight cases determined. This indicates a paternal contribution to trisomy and a female nondisjunction frequency of 1.5% (five of 321). For monosomy where the origin was identified in five of the cases, the male complement was implicated in three zygotes. The single break was seen in a sperm-derived chromosome. Two of the three triploids were dispermic and one was digynic. The outbred Swiss mouse might be a useful model for studying factors responsible for the induction of cytogenetic anomalies in early development of mammal

ISSN:1424-8581    

DOI:10.1159/000131837

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

We have used a cloned EcoRl1.5 kb fragment from the human a2(I) collagen gene (COL1A2) as a probe for detecting this gene in human-mouse somatic cell hybrids. Using hybrids containing an X/7 translocation, we have localized this gene to 7pter→7q2

ISSN:1424-8581    

DOI:10.1159/000131838

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Phosphoserine phosphatase (PSP) catalyzes the hydrolysis of phosphoserine to serine. PSP expression has been examined in human-mouse somatic cell hybrids retaining different combination of human chromosomes. Human PSP is expressed only when the pter→q22 segment of the human 7 and its enzyme marker qβ-glucuronidase (GUSB) are retained in cell hybrids. The structural gene, PSP,is therefore assigned to this region of the human

ISSN:1424-8581    

DOI:10.1159/000131839

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Human phosphoserine phosphatase (E.C. 3.1.3.3) (PSP), a phosphohydrolase, participates in the biosynthesis of serine from carbohydrates (Moro-Furlani et al., 1980). Starch gel electrophoresis has identified isoenzymes and rare allelic variants. More common electrophoretic variation is thought to be due to secondary nongenetic modification. Using starch gel electrophoresis of interspecific somatic cell hybrids, we have been able to assign the gene for this enzyme to human chromosome 7.

ISSN:1424-8581    

DOI:10.1159/000131840

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

There is substantial evidence that many XX males arise from an X/Y interchange, so that a terminal Xp segment carrying the Xg locus, but not the neighboring steroid sulfatase (STS) locus, is replaced by part of the Y chromosome. We show here that one of the two X chromosomes of an XX male with low intracellular levels of STS does not express the STS gene.

ISSN:1424-8581    

DOI:10.1159/000131841

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

Glucocorticoid-resistant variants of L cells were characterized by somatic cell hybridization. Neither of the variants had a clearly dominant phenotype in hybrids with a glucocorticoid-sensitive fibroblast line. Hybrids of one of the resistant variants and a resistant fibroblast line showed complementation — the hybrid clones were sensitive to dexamethasone. Complementation, however, disappeared after continued culture of the hybrid cell

ISSN:1424-8581    

DOI:10.1159/000131842

出版商:S. Karger AG    

年代:1983

数据来源: Karger

摘要:

In a study requiring rapid diagnosis of the chromosomal sex of mice, standard staining methods for mouse chromosomes were evaluated for speed, simplicity, and the quality of staining. None of the techniques previously used for mouse chromosomes were adequate. However, a DAPI-distamycin A method for human karyotyping was successfully adapted for this purpose.

ISSN:1424-8581    

DOI:10.1159/000131843

出版商:S. Karger AG    

年代:1983

数据来源: Karger

ISSN:1424-8581    

DOI:10.1159/000131844

出版商:S. Karger AG    

年代:1983

数据来源: Karger