摘要:

Our previous assignment of the gene loci HBB, HRASl, INS, PTH, LDHA, and CAT to owl monkey chromosome 19 of karyotype VI (K-VI) indicated a putative homology of this owl monkey chromosome with the short arm of human chromosome 11 (HSA 11p). To investigate further the extent of shared homolgy, we localized in the owl monkey complement two genes known to be on HSA 11q. Segregation analysis of ETS1 and THY1 homologous DNA in three karyotypically different panels of rodent × owl monkey somatic cell hybrids provided evidence for the syntenic assignment of these loci to homologous chromosomes of three owl monkey karyotypes, namely, chromosomes 4 (K-VI), 3 (K-II), and 5 (K-V). The results indicate a disruption of syntenic gene loci on the distal portion of HSA 11q from 11p during primate evolution

ISSN:1424-8581    

DOI:10.1159/000132618

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

We report the amplification of the putative oncogene INTl in two of four retinoblastomas. In one case, the INTl signal was amplified 10 to 15-fold, in the other 100-fold. In both cases there were signs of increased tumor aggressiveness with invasion of the choroid and development of metastases. The two cases without INTl amplification had neither metastases nor locally invasive growth. Our findings indicate that INTl amplification may be a feature of increased malignant potential in retinoblastomas.

ISSN:1424-8581    

DOI:10.1159/000132619

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

The pink salmon (Oncorhynchus gorbuscha) has a rigid two year life cycle so that populations spawning on the even years do not hybridize with populations spawning on the odd years. Examination of the chromosomes of two populations from an even year (1986) and four populations from an odd year (1987) showed that all individuals from the even year populations had a diploid number’of 52, considered the normal number for the species, while a high frequency of individuals in each of the odd year populations sampled from Washington State to Alaska were translocation heterozygotes with a diploid chromosome number of 53. The chromosome involved in the translocation was the seventh metacentric pair containing the NOR (nucleolus organizer region) adjacent to the centromere. In two populations a simple fission of this chromosome has produced individuals with chromosomes with two acrocentrics replacing the metacentric chromosome, with the larger acrocentric having the NOR adjacent to the centromere on the long arm. In the other two populations individuals with 53 and 54 chromosomes were found in which the acrocentric with the NOR has undergone an inversion so that the NOR is now on the short arm of a small submetacentric chromosome. In one population all of the individuals with 53 and chromosomes were of this type, while in the other case both forms were found. Because these two populations are adjacent to each other in the middle of the range sampled, the rearranged chromosome probably had a single origi

ISSN:1424-8581    

DOI:10.1159/000132620

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

The mitotic and meiotic chromosomes of four male East-Asiatic mice, Apodemus peninsulae, having three to seven chromosomes in addition to the standard karyotype (2n = 48), were investigated. B-chromosomes were represented by medium-sized metacentric and dotlike chromosomes. Mosaicism of bone marrow cells due to a numerical variation of accessory chromosomes was established for the males examined. Capacity of B-chromosomes to- form axial elements and synaptonemal cornplexes in meiotic prophase I was revealed by electron microscopy. The occurrence of univalents of different morphology, bivalents, and multivalents, corresponding to B-chromosomes, was demonstrated. An increase in the number of B-chromosomes was found in spermatocytes at zygotene-pachytene relative to the number in bone marrow cells, which may be evidence of B-chromosome accumulation in the germ cell line of the East-Asiatic mouse.

ISSN:1424-8581    

DOI:10.1159/000132621

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

Fluorescent in situ hybridization provides a fast method for detection of specific nucleic acid sequences. We have used high-resolution, single-color fluorescent in situ hybridization with a combination centromeric-telomeric DNA probe, specific for chromosome 1, to investigate the feasibility of simultaneous assessment of numerical and structural chromosome aberrations. The K562 leukemia cell line served as a model.

ISSN:1424-8581    

DOI:10.1159/000132622

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

In situ hybridization was used to localize a cDNA probe of the acetyl-CoA carboxylase gene to human metaphase chromosomes. A significant proportion of the grains were situated over chromosome band 17q21. In situ hybridization to a t(6; 17)(p25;q21.33) confirmed the location of the gene proximal to 17q21.33.

ISSN:1424-8581    

DOI:10.1159/000132623

出版商:S. Karger AG    

年代:1988

数据来源: Karger

ISSN:1424-8581    

DOI:10.1159/000132624

出版商:S. Karger AG    

年代:1988

数据来源: Karger