摘要:

We report here a cytogenetic and molecular analysis of two cases of T-cell leukemia with t(14;14) (q11.2;q32). Through in situ hybridization and Southern blotting, using radioactively labeled immunoglobulin heavy chain (IGH) and α T-cell receptor (TCRA) gene probes, we found in both tumors that the loci of both IGH and TCRA were rearranged. Molecular analysis of the t(14; 14) clearly demonstrated that in some tumors rearrangements of the IGH and TCRA genes are associated with interchromosomal exchanges that result in specific chromosome translocations that confer a proliferative advantage and predisposition to leukemic transformation. The implication of these rearrangements for normal and neoplastic T-cell development is discussed

ISSN:1424-8581    

DOI:10.1159/000132918

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Prolactin receptor (PRLR) and growth hormone receptor (GHR) are encoded by members of a gene family containing regions of identical sequences. To determine their chromosomal locations, cDNA probes for these genes were used. Analysis of hybridization to several somatic cell hybrids, together with hybridization in situ to metaphase chromosomes, resulted in the assignment of the loci for both receptors to human chromosome 5 in the region p13→p14. Thus, these proteins may be encoded by a cluster of related genes that evolved from a common ancestral gene, in a manner consonant with that of their ligand

ISSN:1424-8581    

DOI:10.1159/000132919

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Human IFI-15K and 6–16 genes are transcriptionally stimulated by interferons, double-stranded RNA, and viruses. By screening a cDNA library with oligodeoxynucleotide probes, we ave isolated complete copies corresponding to these two genes. These cDNA clones allowed us to localize the IFI-15K and 6–16 genes on human chromosome 1 by somatic cell hybridizat

ISSN:1424-8581    

DOI:10.1159/000132920

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Two human serine protease genes have been cloned. One corresponds to CTLAl, the human equivalent of the mouse cytotoxic cell protease gene Ctla-1, and the other is novel. Both genes were localized to 14q 11.2→q 12 by in situ hybridization. This result confirms the assignment of human CTLAl to 14q11.2→q12 and provides new mapping data for another human serine protease gene located in the same chromosome reg

ISSN:1424-8581    

DOI:10.1159/000132921

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

A panel of hybrid clones segregating rat chromo somes in a mouse background was used to determine the chromo somal localization of three genes specifically expressed in hepato cytes. The phenylalanine hydroxylase, tyrosine aminotransferase, and pyruvate kinase genes were assigned to rat chromosomes 7, 19, and 2, respectively.

ISSN:1424-8581    

DOI:10.1159/000132922

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Gene loci for phosphoglycolate phosphatase and hydroxyacyl glutathione hydrolase were localized within human chromosome band 16pl3.3. This result was determined by the electrophoretic detection of enzymes from a human × mouse somatic cell panel, the members of which carried portions of human chromosome 16 with precisely defined breakpoints

ISSN:1424-8581    

DOI:10.1159/000132923

出版商:S. Karger AG    

年代:1990

数据来源: Karger