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| 1. |
Report of the Second International Workshop on Human Chromosome 2 Mapping 1992 |
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Cytogenetic and Genome Research,
Volume 64,
Issue 2,
1993,
Page 69-92
Nigel K. Spurr,
Susan Naylor,
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ISSN:1424-8581
DOI:10.1159/000133564
出版商:S. Karger AG
年代:1993
数据来源: Karger
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| 2. |
Report of the Second International Workshop on Human Chromosome 9 Mapping 1993 |
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Cytogenetic and Genome Research,
Volume 64,
Issue 2,
1993,
Page 93-121
David J. Kwiatkowski,
Sue Povey,
Jonathan L. Haines,
Margaret Pericak-Vance,
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PDF (6987KB)
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ISSN:1424-8581
DOI:10.1159/000133566
出版商:S. Karger AG
年代:1993
数据来源: Karger
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| 3. |
A mouse stock with 38 chromosomes derived from the reciprocal translocation T(7;15)33Ad |
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Cytogenetic and Genome Research,
Volume 64,
Issue 2,
1993,
Page 122-127
G. Schriever-Schwemmer,
I.-D. Adler,
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PDF (1153KB)
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摘要:
A reciprocal translocation, T(7;15)33Ad, with presumed breakpoints in bands 7A1 and 15F3 was induced in late spermatids by injecting male (102/E1 × C3H/E1)F! mice five times with acrylamide (50 mg/kg body weight). Outcrosses of the original semisterile T(7; 15) female generated three males monosomic for the short marker 715 [Ms(715)] among a total of 15 males. The Ms(715) males sired small litters and had reduced testes weights. From inter se matings of Ms(715) animals, nullisomic progeny for chromosome 715 were obtained and mated to produce a breeding stock of mice with 38 chromosomes. For comparison, mice carrying the reciprocal translocation T(4;8), with similarly located breakpoints, were also analyzed. Fluorescent in situ hybridization (FISH) with major and minor satellite DNA probes and a telomeric DNA probe was utilized. The observed FISH signals suggest that in chromosomes 7 and 8 the breaks occurred within the pericentric heterochromatic block immediately below the centromere and in chromosomes 15 and 4 at a point near the distal telomeres. The long markers 157 and 48 are tandem fusion chromosomes. The short markers 715 and 84 also showed all appropriate FISH signals for intact chromosomes. The loss of the small chromosome 715 was compatible with survival, suggesting that no essential genes are located on the small reciprocal translocation product. The development of this tandem fusion stock is described as a laboratory example of one possible step in karyotypic evolution
ISSN:1424-8581
DOI:10.1159/000133567
出版商:S. Karger AG
年代:1993
数据来源: Karger
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| 4. |
Identification and characterization of a cDNA, which is highly homologous to the ribonucleoprotein gene, from a locus (D1OS102) closely linked to MEN2 (multiple endocrine neoplasia type 2) |
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Cytogenetic and Genome Research,
Volume 64,
Issue 2,
1993,
Page 128-130
S. Takiguchi,
T. Tokino,
T. Imai,
A. Tanigami,
K. Koyama,
Y. Nakamura,
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PDF (687KB)
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摘要:
We previously reported a nearly 1 -Mb contig of cosmid and YAC clones at D10S102, a locus that is tightly linked to the gene responsible for multiple endocrine neoplasia type 2 (MEN2A). Subclones of 159 genomic EcoRlfragments in the contig were tested on “zoo blot” to detect DNA sequences conserved in various species. Nine fragments found to contain conserved sequences were used as probes for screening cDNA libraries. One of these probes, y300-pL50, recognized a cDNA from a human fetal brain library. The cDNA encoded a protein consisting of 270 amino acids, whose predicted sequence has a high degree of homology to heterogeneous nuclear ribonuclear protein. This expressed sequence may be a candidate for the gene responsible for ME
ISSN:1424-8581
DOI:10.1159/000133568
出版商:S. Karger AG
年代:1993
数据来源: Karger
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