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| 1. |
Assignment of C0X6A1 to 6p21 and a pseudogene (C0X6A1P) to 1p31.1 by in situ hybridization and somatic cell hybrids |
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Cytogenetic and Genome Research,
Volume 77,
Issue 3-4,
1997,
Page 167-168
Y. Hey,
N. Hoggard,
E. Burt,
L.A. James,
J.M. Varley,
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PDF (264KB)
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ISSN:1424-8581
DOI:10.1159/000134565
出版商:S. Karger AG
年代:1997
数据来源: Karger
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| 2. |
Human type I cytokeratin genes are a compact cluster |
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Cytogenetic and Genome Research,
Volume 77,
Issue 3-4,
1997,
Page 169-174
N. Ceratto,
C. Dobkin,
M. Carter,
E. Jenkins,
X.-L. Yao,
J.-J. Cassiman,
M.S. Aly,
P. Bosco,
R. Leube,
L. Langbein,
S. Feo,
V. Romano,
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摘要:
A YAC clone (211F11) containing approximately 0.5 Mb of human DNA was isolated from a human genomic library by PCR-based screening with cytokeratin (KRT) 13-specific primers. The YAC clone was mapped by FISH to the long arm of chromosome 17 (17q12→q21), a region to which several other type I KRT genes had been mapped previously. We now show by Southern blot hybridization and PFGE analyses that KRT13, 14, 15, and 16 are all contained within YAC clone 211F11. Long-range restriction mapping analysis of clone 211F11 and of two smaller YAC clones that were also isolated with KRT13-specific primers, suggests that KRT13, 14, 15, 16 and their linked type I genes KRT17 and 19, are contained in less than 150 kb of genomic DNA. According to our reconstruction it then appears that at least six type I KRT genes are arranged in a highly compact cluster. The three YACs reported in this study represent a new tool to dissect the molecular structure of the locus of the human type I KRT gene
ISSN:1424-8581
DOI:10.1159/000134566
出版商:S. Karger AG
年代:1997
数据来源: Karger
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| 3. |
A FISH probe specific for the telomeric region of 6p |
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Cytogenetic and Genome Research,
Volume 77,
Issue 3-4,
1997,
Page 175-175
G. Mirza,
A.F. Davies,
J. Ragoussis,
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PDF (108KB)
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ISSN:1424-8581
DOI:10.1159/000134567
出版商:S. Karger AG
年代:1997
数据来源: Karger
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| 4. |
Assignment of Acetyl-CoA Carboxylase-β (ACACB) to human chromosome band 12q24.1 by in situ hybridization |
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Cytogenetic and Genome Research,
Volume 77,
Issue 3-4,
1997,
Page 176-177
C.K. Ullrich,
J. Widmer,
J.P. Park,
T.K. Mohandas,
L.A. Witters,
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PDF (176KB)
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ISSN:1424-8581
DOI:10.1159/000134568
出版商:S. Karger AG
年代:1997
数据来源: Karger
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| 5. |
Assignment of the germ cell cyritestin gene 2 (CYRN2) to human chromosome 16q12.1 by fluorescence in situ hybridization to 5-azacytidine induced, partially decondensed chromosomes |
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Cytogenetic and Genome Research,
Volume 77,
Issue 3-4,
1997,
Page 178-179
M.R. Koehler,
G. von Beust,
W. Engel,
M. Schmid,
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PDF (210KB)
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ISSN:1424-8581
DOI:10.1159/000134569
出版商:S. Karger AG
年代:1997
数据来源: Karger
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| 6. |
Assignment of the mouse heme oxygenase genes: heme oxygenase-1 (Hmox1)to chromosome 10 band C1 and heme oxygenase-2 (Hmox2)to chromosome 16 band B1 |
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Cytogenetic and Genome Research,
Volume 77,
Issue 3-4,
1997,
Page 180-181
F. Saito-Ohara,
T. Ikeuchi,
M. Matsumoto,
S. Kurata,
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PDF (180KB)
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ISSN:1424-8581
DOI:10.1159/000134570
出版商:S. Karger AG
年代:1997
数据来源: Karger
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| 7. |
Minibrain (MNBH) is a single copy gene mapping to human chromosome 21q22.2 |
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Cytogenetic and Genome Research,
Volume 77,
Issue 3-4,
1997,
Page 182-184
J. Guimera,
M. Pritchard,
M. Nadal,
X. Estivill,
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PDF (537KB)
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摘要:
We have identified a human homologue of the Drosophila mnb gene (MNBH) on chromosome 21, while another study mapped an EST clone (R38268) with similarity to minibrain to human chromosome 1. This report describes the mapping of MNBH to a single locus on human chromosome 21q22.2 by FISH and Southern blotting. Comparison of the similarities between the two sequences and mnb demonstrates that MNBH on chromosome 21 is the true human homologue of Drosophila mnb.
ISSN:1424-8581
DOI:10.1159/000134571
出版商:S. Karger AG
年代:1997
数据来源: Karger
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| 8. |
Isolation and mapping of a human zinc finger gene (ZNF188) homologous to ZNF187, a serum-response-element binding protein |
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Cytogenetic and Genome Research,
Volume 77,
Issue 3-4,
1997,
Page 185-189
S. Ishikawa,
M. Kai,
Y. Takei,
K. Okui,
T. Takahashi,
M. Suzuki,
M. Ogawa,
Y. Nakamura,
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PDF (831KB)
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摘要:
From a human pancreas cDNA library we isolated and characterized a novel zinc finger gene encoding a protein homologous to ZNF187, a serum response element-binding protein. The full-length cDNA contained an open reading frame of 1,686 nucleotides encoding a predicted 562-amino-acid peptide that included an ATP-GTP binding site and seven C2H2 zinc finger domains. The consensus sequence of the C2H2 domains (CX2CX3FX5LX2HX3H) is common in the SRE-binding region present in Drosophila Krüppel proteins. An alternatively spliced form of the transcript found in the cDNA library lacked both the ATP-GTP binding site and any C2H2 zinc finger domains. We localized this gene (ZNF188) to chromosome band 7q22.1→q22.3 by fluorescence in situ hybridizati
ISSN:1424-8581
DOI:10.1159/000134572
出版商:S. Karger AG
年代:1997
数据来源: Karger
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| 9. |
Assignment of the L32 ribosomal protein gene (RPL32) to human chromosome 3q13.3→q21 by in situ hybridization |
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Cytogenetic and Genome Research,
Volume 77,
Issue 3-4,
1997,
Page 190-191
N.V. Vorobieva,
M.L. Filipenko,
G.G. Karpova,
N.P. Mertvetsov,
A.S. Graphodatsky,
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PDF (227KB)
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ISSN:1424-8581
DOI:10.1159/000134573
出版商:S. Karger AG
年代:1997
数据来源: Karger
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| 10. |
Microdissection-mediated selection of chromosome region-specific cDNAs |
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Cytogenetic and Genome Research,
Volume 77,
Issue 3-4,
1997,
Page 192-196
Y. Yokoyama,
K. Ohsugi,
T. Kozaki,
N. Sakuragawa,
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PDF (956KB)
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摘要:
K562 is a cell line with two acrocentric marker chromosomes containing abnormally banded regions (ABRs), derived from a Ph-positive chronic myelogenous leukemia (CML) patient. Using reverse and forward chromosome painting FISH analysis, we found that 9q34, 13q31, and 22q11 regions co-amplified in the ABRs-bearing acrocentric marker chromosomes of K562. Utilizing the ABRs of the cell line as target DNA for cDNA selection, we established a simple procedure for chromosome region-specific cDNA isolation. After first strand cDNA synthesis from fetal brain mRNAs, short fragment cDNAs (sf-cDNAs) were synthesized with a two-step amplification system by use of our modified Degenerate Oligonucleotide Primed Shuttle Polymerase Chain Reaction (DOP-Shuttle-PCR) method. The sf-cDNAs were hybridized onto RNase A treated metaphases from K562, and the ABRs were microdissected and reamplified with DOP-Shuttle-PCR primer-II. The reamplified sf-cDNAs were cloned into a pBluescript vector. Twenty randomly chosen clones were sequenced and classified into 8 groups. Three out of the 8 grouped clones had been mapped to the long arm of chromosome 22 (22q11), whereas the other 5 were novel cDNAs. Quantitative Southern blot analysis indicated that 7 out of the 8 grouped clones (87.5%) were derived from the co-amplified regions.
ISSN:1424-8581
DOI:10.1159/000134574
出版商:S. Karger AG
年代:1997
数据来源: Karger
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