ISSN:1424-8581    

DOI:10.1159/000133982

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

We have sub localized to the region between 1p22 and lp33 a total of 14 yeast artificial chromosomes previously assigned to a broader area of human chromosome 1p. Our purpose was to map DNA sequences that could be used for the molecular characterization of the two common fragile sites present in bands 1p31.2 and 1p32, the expression of which is increased in patients with neuroblastomas.

ISSN:1424-8581    

DOI:10.1159/000133983

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

While constructing a contig in the human chromosome region 3q27, we identified two YAC clones that were positive for the polymorphic marker D3S1602. One of these clones was also positive for a sequence-tagged site derived from the kininogen (KNG) gene. Because of the known evolutionary and structural relationship of KNG to other members of the cystatin gene superfamily, we tested the physical linkage of the genes encoding alpha-2HS-glycoprotein (AHSG), KNG, and histidine-rich glycoprotein (HRG), all of which were previously mapped to the long arm of chromosome 3. Our results show the colocalization of the three genes in two independent, partially overlapping YAC clones. The genomic inserts of the two clones were 1 Mb and 1.3 Mb in size, indicating that the three genes map within 1 Mb of DNA. The largest YAC was also positive for the polymorphic marker D3S1262, substantiating previously reported data of genetic linkage between this marker and HRG. Fluorescence in situ hybridization localized the two YAC clones to chromosome band 3q27.

ISSN:1424-8581    

DOI:10.1159/000133984

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Cytogenetic investigation of three species of the genus Gymnophthalmus (the unisexual G. underwoodi, G. leucomystax and Gymnophthalmus sp.n., a new, undescribed redtailed species) documented three different karyotypes, all with 2n = 44. Chromosomes from fibroblast cultures were studied after routine Giemsa staining, CBG-banding, RBG-banding, and Ag-NOR staining. The unisexual species showed a very distinctive karyotype from the one previously described that, paradoxically, corresponded to the predicted karyotype of one of its parental species. Our chromosome data unequivocally show that there are two parthenogenetic species with two different mechanisms of origin under the name underwoodi.

ISSN:1424-8581    

DOI:10.1159/000133985

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

We describe the generation of large-fragment microclone libraries from the chromosomal breakpoint of a reciprocal balanced translocation linked to schizophrenia. The abnormality was visible under the phase-contrast microscope, allowing direct dissection from unstained, unhanded met-phases. Two separate microdissection experiments yielded 443 and 672 recombinants, respectively. Following complete EcoRl digestion, inserts with an average size of 0.3 kb (range, 0.2–3 kb) were obtained in the first experiment and 1.5 kb (range, 0.15–6.5 kb) in the second. FISH analysis of pooled clones “painted” back onto the derivative chromosome and assignment of microclones to somatic cell hybrids confirmed the fidelity of the method. Microdissection of chromosome regions identified by karyotype rearrangements in unstained, unhanded metaphases is a potentially powerful tool for positional

ISSN:1424-8581    

DOI:10.1159/000133986

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Using fluorescence in situ hybridization techniques with either human or hamster genomic DNA probes, we studied the origin of micronuclei in two-cell hybrid embryos obtained from hamster oocytes and γ-irradiated human spermatozoa. Our study demonstrates that over 99 % of micro nuclei hybridize with human DNA probes and not with hamster DNA, revealing their human origin. Thus, the micronucleus test represents a good method to evaluate genetic damage in human germ cells, since it is simpler and faster than sperm chromosome studies

ISSN:1424-8581    

DOI:10.1159/000133987

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Dehydroepiandrosterone (DHEA) sulfotransferase (ST) catalyzes the sulfate conjugation of DHEA and other steroid compounds. The human gene for DHEA ST (STD) was mapped by the polymerase chain reaction to chromosome 19 using human × rodent somatic cell hybrid panels. Fluorescence in situ hybridization was then used to localize the STD gene to the region 19q13.3

ISSN:1424-8581    

DOI:10.1159/000133988

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

The murine invasion-inducing Tiaml gene maps to the distal end of chromosome 16, 3.8 cM centromeric of the Ets2 gene. TIAM1, the human homolog of Tiaml, maps to the syntenic region (q22) on human chromosome 21. The gene order in 21q22 is cen–TIAM1–AML1–ERG–E

ISSN:1424-8581    

DOI:10.1159/000133989

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Human protein tyrosine phosphatase, receptortype, zeta (PTPRZ; also denoted HPTPζ or RPTPβ) has a large extracellular region with the N-terminal carbonic anhydrase-like domain and a cytoplasmic region with two tandemly located protein tyrosine phosphatase domains. One of the characteristics of PTPRZ is its preferential expression in the central nervous system. We localized the human PTPRZ gene to chromosome band 7q31.3 by somatic cell hybrid mapping and fluorescence in situ hybridizatio

ISSN:1424-8581    

DOI:10.1159/000133990

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Using fluorescence in situ hybridization method, the gene encoding the protein phosphatase type 1α catalytic subunit (PPP1CA) was localized to human chromosome band 11q13, rat chromosome band 1q43, and mouse chromosome band 7E3-F2. These results suggest that PPP1CA is a member of asyntenic group

ISSN:1424-8581    

DOI:10.1159/000133991

出版商:S. Karger AG    

年代:1995

数据来源: Karger