ISSN:1424-8581    

DOI:10.1159/000134027

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

The three loci NRAS, NGFB, and CD2 map toband 1p13.2 and CD2 and NGFB to human chromosome band 1p13. Using fluorescence in situ hybridisation (FISH) to simultaneously DAPI-banded metaphase chromosomes, we have further refined the localisation of these three genes to specific subbands. NRAS localises to sub-1p13.1. Also, with the use of multicolour FISH, we have determined the order and orientation of the three loci in relation to the centromere. The orders cen-CD2-NGFB-NRAS.

ISSN:1424-8581    

DOI:10.1159/000134028

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

The porcine gene for α1-acid glycoprotein (ORM1) was localized to chromosome region 1q210→ q212 by fluorescence in situ hybridization. From this result and published gene mapping data, it was concluded that the syntenic group comprised of ORM1, IFNA1, and GRP78 in the human genome is also conserved in the p

ISSN:1424-8581    

DOI:10.1159/000134029

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

The gene loci for human CEBPD (CCAAT enhancer binding protein, δ chain) and FGFR1 (fibroblast growth factor receptor) have been identified within two genetically mapped cosmids by sequence homology between rare cutter site regions and data base sequences for these loci. Cell hybrid and fluorescence in situ hybridization mapping places both of these loci within the chromosome region 8p11.2→p11

ISSN:1424-8581    

DOI:10.1159/000134030

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

A clone carrying an open reading frame coding for a novel zinc finger protein of the Krüppel family was isolated from a bovine genomic library and designated ZNF164 (zinc finger protein 164). Partial sequencing revealed that it contained at least 13 zinc finger motifs preceded by a lysine-rich region of 60 amino acids. The ZNF164 protein shared -60% similarity with several zinc finger proteins but did not appear to be orthologous with a previously identified gene. Using fluorescence in situ hybridization, the ZNF164 gene was mapped to bovine chromosome band 17q24

ISSN:1424-8581    

DOI:10.1159/000134031

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

S-adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in polyamine biosynthesis. The human genome contains at least two loci for the AdoMetDC gene (AMD), one of which (AMD1) has previously been mapped to chromosome 6 and the other (AMD2) to the X chromosome. The locus on chromosome 6 is the transcriptionally active gene. We now report characterization of the AMD2 locus (GenBank Accession No. U02035) on the X chromosome, which contains sequences that cross-hybridize with human AdoMetDC cDNA. This DNA lacks all of the introns present in AMD1 and has numerous mutations in the protein-coding region. Its overall nucleotide sequence identity with AdoMetDC cDNA is about 90%. AMD2 is therefore a processed pseudogene, which, because of multiple mutations, cannot be translated to an active AdoMetDC enzyme, even if it were transcribed. Chromosomal loci for human AMD sequences were determined by in situ hybridization to metaphase chromosomes, with genomic DNAs from the active gene and the pseudogene loci as probes. AMD1 was localized to chromosome region 6q21→q22 and AMD2 to band Xq2

ISSN:1424-8581    

DOI:10.1159/000134032

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

The mouse cDNA encoding the major protein of the outer dense fibers in sperm tails was isolated by reverse transcription of testicular RNA and amplification with sequence-specific primers. Sequencing of a genomic clone obtained by inverse PCR yielded the 5’ untranslated region. The transcription starting point was verified by primer extension. The putative proteins encoded by Odfl in mouse and by ODF1 in rat and man are very similar. A total of 15 amino acids in the C-terminal region were deleted in the mouse protein, compared with the rat protein. Through in situ hybridization to metaphase chromosomes, the Odfl gene was localized to mouse chromosome 15 region B2-C. The chromosomal localization of the Odfl gene extends the hitherto known linkage group consisting of MYC (Myc), PVT1 (Pvt1), GPT (Gpt), and TG (Tg) common to human chromosome 8 and mouse chromosome 15 in the proximal direction of both chromosomes. The linkage group now extends from band q24 to band q22 of human chromosome 8 and from region D2-E to region B2-C of mouse chromosome 1

ISSN:1424-8581    

DOI:10.1159/000134033

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

We have used chromosome 18-specific painting probes to analyze early stages of oogenesis in two human trisomy 18 fetuses. At leptotene, a diffuse, nonlinear chromosomal fluorescence was detected as one (27%), two (42%), or three (31 %) signals in 534 cells. The variation in size of these signals implies the possibility of associations between homologs prior to zygotene. At pachytene, about 75 % (339/453) of the cells had a trivalent configuration, and almost half of these cells exhibited almost complete triple synapses. Approximately 24% of the pachytene cells demonstrated a bivalent:univalent configuration, and 1 % exhibited complete asynapsis. Our data imply that triple synapses may be a regular feature of meiosis involving multivalents.

ISSN:1424-8581    

DOI:10.1159/000134034

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

A cDNA representing the high-affinity gonadotropin-releasing hormone (GnRH) receptor has been molecularly cloned from the human pituitary gland, a breast tumor cell line (MCF 7), and an ovarian tumor. The nucleotide sequence of this cDNA was determined, and its expression in various human tumors and tumor cell lines was demonstrated. In this study, we localized the gene encoding the GnRH receptor to human chromosome 4, using polymerase chain reaction (PCR) analysis of genomic DNA from human × hamster somatic cell hybrids. The gene was sublocalized to chromosome band 4q13 using fluorescence in situ hybridization with the GnRH receptor gene (GNRHR)

ISSN:1424-8581    

DOI:10.1159/000134035

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

We have isolated a human cDNA clone homologous to the canine beta-signal sequence receptor gene, which codes for an endoplasmic reticulum (ER) membrane protein associated with protein translocation across the ER membrane. Northern blot analysis revealed its ubiquitous expression in all organs examined. We also localized the human beta-signal sequence receptor gene (SSR2) to chromosome bands 1q21→q23 by fluorescence in situ hybridizatio

ISSN:1424-8581    

DOI:10.1159/000134036

出版商:S. Karger AG    

年代:1995

数据来源: Karger