ISSN:1424-8581    

DOI:10.1159/000133536

出版商:S. Karger AG    

年代:1993

数据来源: Karger

摘要:

Otf-3 (Octamer-binding transcription factor 3) is an octamer binding protein encoded by the murine gene Otf-3. Otf-3 belongs to a multigenic family and maps to the mouse Chromosome 17 between the Q and T regions within the major histocompatibility complex (MHC). We report the mapping of the human homologue: OTF3, to human chromosome 6p21.3→p22 within or close to the human MHC class I region. Furthermore, one OTF3-like copy (OTF3L) is localized to 12p1

ISSN:1424-8581    

DOI:10.1159/000133537

出版商:S. Karger AG    

年代:1993

数据来源: Karger

摘要:

Cytogenetic analysis of two local populations of Microlepidogaster leucofrenatus showed a basic diploid chromosome number (2N) of 54 in both populations. Some fishes were found to have a 2N = 55 or 56 chromosomes due to the presence of one or two large heterochromatic B chromosomes. Specimens of M. leucofrenatus from the Poço Grande stream had 24 metacentrics, 24 submetacentrics, four subtelocentrics, and one submetacentric homomorphic pair in males and one submetacentric/subtelocentric heteromorphic pair in females, whereas individuals of this species from the Marumbi River had 22 metacentrics, 24 submetacentrics, four subtelocentrics, two acrocentrics, and one submetacentric/subtelocentric heteromorphic pair in females. The occurrence of the heteromorphic pair in the females was due to the presence of an extra C-banded segment on the W chromosome. Ag-NORs in both populations were located interstitially on the short arm of the largest metacentric pair. The Poço Grande population had less constitutive heterochromatin than did the Marumbi River population. The speciation process in this fish species is discussed on the basis of heterochromatin distributio

ISSN:1424-8581    

DOI:10.1159/000133538

出版商:S. Karger AG    

年代:1993

数据来源: Karger

摘要:

Light- and electron-microscopic analyses of chromosomal pairing and recombination in F1 and first-backcross generation mice of the C57BL/6J × Mus spretus cross revealed a variety of meiotic irregularities that could contribute to meiocyte loss and infertility. Pachytene anomalies included univalency, partially paired bivalents, homolog-length inequalities, nonhomologous pairing, and associations of asynapsed autosomal segments with the X chromosome. These phenomena were most prevalent in Fi males, which are invariably sterile. Although F[ females were qualitatively fertile, breeding data indicated significant reproductive impairment. Molecular analyses of X-linked and pseudoautosomal loci in sterile and fertile backcross males revealed that the failure of X-Y pairing and recombination is correlated with heterozygosity within the pseudoautosomal regions of the X and Y chromosomes. In addition to impairing fertility, the synaptic disturbances (such as localized asynapsis and nonhomologous pairing) observed in F1 individuals can potentially alter recombinational patterns, thereby contributing to the genetic-map distortion observed with this interspecific cross. Together, the cytogenetic and reproductive data suggest that sex-related differences in the gametogenic process, quantitative differences in the incidence of synaptic irregularities in female and male meiosis, and phenomena associated with the X and Y chromosomes comprise the etiological basis of the sex-biased F1 sterility. The differential gender-related effects of these cytogenetic phenomena may constitute the underlying basis of Haldane’s rule in mamma

ISSN:1424-8581    

DOI:10.1159/000133539

出版商:S. Karger AG    

年代:1993

数据来源: Karger

摘要:

A new version of our previously published PRINS (PRimed IN Situ labeling) method is presented. It represents a significant improvement in the detection of specific DNA sequences in situ. The idea is to perform the reaction repeatedly, rather than just once. This change of strategy results in a localized accumulation of sequence-specific labeled DNA, resulting in up to a 15-fold amplification of the signal as compared to the standard PRINS method. Interestingly, the retention of the labeled DNA is so good that it stays within the chromosomal band where it is synthesized, provided that the reaction is not performed an excessive number of times. The key trick is the performance of the procedure on small glass slides in PCR tubes, thereby avoiding the use of cover slips. In addition, use of the small glass slides seems to give less variance when the signals are quantified in a fluorescence microscope.

ISSN:1424-8581    

DOI:10.1159/000133540

出版商:S. Karger AG    

年代:1993

数据来源: Karger

摘要:

The human genes encoding the interleukin 8 receptors type A and B were assigned to chromosome 2 by polymerase chain reaction amplification and by Southern analysis of a panel of human × rodent somatic cell hybrid DNAs. The IL8R genes were further localized by in situ hybridization to band 2q35

ISSN:1424-8581    

DOI:10.1159/000133541

出版商:S. Karger AG    

年代:1993

数据来源: Karger

摘要:

Human erythrocyte integral membrane protein band 7 (also termed protein 7.2b or stomatin) is involved in the Na+/K+ permeability of red cells. A cDNA clone coding for this protein was used as a probe to determine the chromosomal localization of the gene (EPB72). Southern blot analysis of somatic cell hybrid DNA panels localized the human EPB72 gene to chromosome band 9q34.1.

ISSN:1424-8581    

DOI:10.1159/000133542

出版商:S. Karger AG    

年代:1993

数据来源: Karger

摘要:

The applicability of fluorescence in situ DNA hybridization to hair root preparations for the detection of numerical chromosome anomalies is demonstrated. This simple method yields a high number of scorable nuclei, allowing rapid identification of epithelial cell chromosome composition. In clinical genetics, this technique may become useful in cases where other types of cells are difficult to obtain.

ISSN:1424-8581    

DOI:10.1159/000133543

出版商:S. Karger AG    

年代:1993

数据来源: Karger

ISSN:1424-8581    

DOI:10.1159/000133544

出版商:S. Karger AG    

年代:1993

数据来源: Karger

ISSN:1424-8581    

DOI:10.1159/000133545

出版商:S. Karger AG    

年代:1993

数据来源: Karger