ISSN:1424-8581    

DOI:10.1159/000134055

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

We developed an improved, simple method of generating chromosome-region-specific probes from only a few microdissected chromosomes. One to five dissected fragments from a defined chromosomal region were processed with a PEG/proteinase K cycling deproteinization step and directly amplified with a two-step amplification system using a degenerate oligonucleotide primed shuttle polymerase chain reaction (DOP-Shuttle-PCR). This modified method offered three advantages over previously reported methods: relaxation of the highly condensed chromosomal DNA, reduction of the risk of endogenous and exogenous contamination, and high efficiency amplification of template DNA. High intensity in the fluorescence in situ hybridization (FISH) signals from normal metaphase chromosomes, as well as regional specificity of these probes, corresponding to regions on R-banded chromosomes, were observed.

ISSN:1424-8581    

DOI:10.1159/000134056

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Physical gene mapping by in situ hybridization is a difficult task in an all-acrocentric mouse karyotype, because all of the chromosomes are morphologically very similar. These difficulties can be overcome by using the many different meta-centric Robertsonian translocation (Rb) chromosomes derived from wild mice. Here we describe the establishment of two Moloney murine leukemia virus-transformed suspension cell lines, WMP-1 and WMP-2, derived from wild mice of the strain WMP/WMP. These mice carry nine pairs of metacentric Rb chromosomes containing chromosomes 1 to 18. Chromosome 19 and the sex chromosomes are the only acrocentric chromosomes. Furthermore, a heterozygous reciprocal translocation between chromosomes 13 and 17 involved in two Rb chromosomes is present in this stock and provides additional marker chromosomes. The chromosome designation of these mice is Rb(10.17)9MplRb(13.15)10MplT(13.17)1Lub.

ISSN:1424-8581    

DOI:10.1159/000134057

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

From a human fetal lung cDNA library we isolated and characterized a human cDNA highly homologous to Rp8, a rat gene associated with programmed cell death. This cDNA, termed PDCD2 (programmed cell death-2), contained an open reading frame of 1032 nucleotides encoding 344 amino acids; it revealed 81% identity in DNA sequence and 83% identity in amino acid sequence with rat Rp8. The gene represented by PDCD2 was expressed in all human tissues examined. We assigned the PDCD2 locus to chromosomal band 6q27 by fluorescence in situ hybridization (FISH).

ISSN:1424-8581    

DOI:10.1159/000134058

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Previously isolated human placental cDNA clones represent a fusion of specific RNA sequences encoded by two genes: lysosomal chitobiase (CTB) and G-protein γ5 subunit (GNG5, Fisher and Aronson, 1992a, 1992b). Both genes have now been mapped to 1p by PCR analysis of somatic cell hybrids and further refined to 1p22 by fluorescence in situ hybridization (FISH) using a YAC clone that contains both the chitobiase and 75 genes

ISSN:1424-8581    

DOI:10.1159/000134059

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

To understand the mechanisms that affect aneuploidy, fluorescence in situ hybridization (FISH), using chromosome-specific centromeric probes, was employed to screen a large population of human sperm for numerical errors. To determine the true rate of disomy for chromosomes 1, 12, 15, and 18, two-color FISH was performed, and for the gonosomes, three-color FISH. The use of multiple, differently colored probes allows one to distinguish a true disomic sperm from a diploid cell. A minimum of 10,000 sperm nuclei from each of five donors was scored per set of centromeric probes, giving a total of 165,330 sperm nuclei. The disomy frequencies for autosomes 1, 12, 15, and 18 were found to be similar, with a mean of 0.10% (range, 0.05%–0.16%) for chromosome 1, 0.16% (0.10%–0.25%) for chromosome 12, 0.11% (0.07%–0.20%) for chromosome 15, and 0.11% (0.08%–0.17%) for chromosome 18. For the sex chromosomes, the mean frequency of disomy was found to be 0.43% (range, 0.23%–0.71%), with XX disomy accounting for 0.07% (0.03%–0.10%), YY disomy 0.21% (0.10%–0.43%), and XY disomy 0.15% (0.08%–0.24%). The incidence of disomic sperm for the sex chromosomes was significantly increased, compared to the frequency of disomy for the autosomes (χ2 = 218.61 P < 0.0001). Diploidy was observed in 0.05%-0.47% of the sperm nuclei counted. Interdonor heterogeneity for disomy frequencies was found to exist for the sex chromosomes and for chromosomes 1 and 15, suggesting significant variation

ISSN:1424-8581    

DOI:10.1159/000134060

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

The human α-synuclein gene (SNCA) was previously identified as the non-Aβ component of Alzheimer’s disease amyloid precursor (NACP). A cosmid clone containing this gene has been isolated and mapped by FISH and high-resolution fluorescence banding to human chromosome 4q21.3

ISSN:1424-8581    

DOI:10.1159/000134061

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Human and mouse cDNAs for a 265-kDa protein which binds specifically to phosphorylated cAMP-response element binding protein (CREB), have recently been isolated. A cDNA probe for the mouse CREB binding protein was used to localize this gene (CREBBP) to human chromosome 16p13.3 by fluorescence in situ hybridization (FISH).

ISSN:1424-8581    

DOI:10.1159/000134062

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Sequence-tagged sites (STSs) are key elements in efforts to construct and integrate the various physical maps of a genome. We report the development of 21 STSs assigned to cosmids mapped to specific locations on human chromosome 19 by fluorescence in situ hybridization (FISH). At least one STS has been assigned to each band. The PCR primers generate a single product from genomic DNA. Each product is a single copy probe for Southern blots of human genomic DNA. YACs have been identified with 18 of these STSs.

ISSN:1424-8581    

DOI:10.1159/000134063

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Comparative gene mapping analysis in mammals suggests that the major histocompatibility complex (MHC) genes map syntenic with genes, such as glyoxylase 1 (GLO1). In man, the MHC locus and other genes of this syntenic group map to chromosome band 6p21.3, and in mouse, these genes map to chromosome 17. In the hamster, however, only the GLOl gene has been localized; GLO1 maps to chromosome 1, suggesting that the genes within the MHC locus also map to this chromosome. We have localized the hamster MHC class I genes to chromosome band 1q17→q18 by fluorescence in situ hybridization (FISH). These results suggest that GLOl and other syntenic genes also lie within this chromosome regio

ISSN:1424-8581    

DOI:10.1159/000134064

出版商:S. Karger AG    

年代:1995

数据来源: Karger