ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1994.tb16138.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1994.tb16139.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1994.tb16140.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryA proposed role for antigen‐presenting dermal dendrocytes in the pathogenesis of many dermal inflammatory skin diseases remains speculative. We therefore sought to determine the phenotype and functional characteristics of antigen‐presenting cells isolated from normal human dermis. Normal adult human skin was incubated overnight with dispase at 4°C, the epidermis was removed, and the residual dermal preparation was then minced and digested with a mixture of hyaluronidase, collagenase, and DNAase at 37°C, prior to filtration through mesh. Dermal cell suspensions thus obtained were stained using specific monoclonal antibodies, and analysed by fluorescence micro‐ scopy or flow cytometry. Mean values were as follows: CD45+leucocytes 39%, HLA‐DR+cells 39%,Ulex europaeusagglutinin I+endothelial cells 26%, CD1a+cells 3.9%, CD11b+cells 16%, CDllc+cells 6%. Mitomycin C‐treated crude dermal cell suspensions induced allostimulation of peripheral blood mononuclear cells in a 7‐day culture, as assessed by3H‐TdR incorporation. Depletion of CDla+Langerhans‐like cells from the dermal cell preparation, by 95, 74 and 90% in three separate experiments using immunomagnetic beads, reduced3H‐TdR incorporation at optimal responder‐to‐ stimulator cell ratios by 90, 64, and 87%, respectively. Our findings suggest that, in normal human dermis, the great majority of the alloantigen‐presenting capacity resides in the CDla+Langerhans cell‐like dendritic antigen‐presenting cell population, and not to any great extent in either CDla−macrophage‐like cells, or HLA‐DR+endothelial cells. The relationship of the CDla+dermal antigen presenting cells to the Langerhans ce

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1994.tb08451.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryCeramides (sphingolipids) are the main polar lipids of the stratum corneum and play an important role in skin barrier function, cell adhesion and epidermal differentiation. In view of the function of ceramides in normal skin, this study aimed to assess their levels in patients with various types of hereditary ichthyosis, in which epidermal homeostasis is markedly abnormal. Stratum corneum samples were collected from 80 patients and 23 normal controls, and the intercellular and lipid envelope ceramides were analysed by high‐performance thin‐layer chromatography. The covalently bound ceramides (ceramides A and B) of the lipid envelope were present in all patients studied, and showed no significant differences from control samples. Total ceramides (ceramides 1–6) were decreased in bullous ichthyosiform erythroderma, which is presumably a secondary phenomenon similar to that seen in patients with atopic dermatitis. Patients with non‐erythrodermic lamellar ichthyosis showed a marked decrease in levels of the important acylceramide, ceramide 1, whereas those with other types of autosomal recessive ichthyosis (limited lamellar ichthyosis and non‐bullous ichthyosiform erythroderma) had mean levels similar to the controls. Ceramide 1 deficiency may therefore define a subgroup within the autosomal recessive ichthyoses. Sjögren‐Larsson syndrome (SLS) shows a deficiency of both acyl‐ceramides (ceramides 1 and 6), which would seem likely to disrupt the normal skin barrier function. Furthermore, glucosyl‐ceramides (cerebrosides) are known to be deficient in the neural tissue of patients with SLS. The relationship of these ceramide abnormalities to the underlying fatty alcohol oxidoreductase defect remains uncertain, but they may provide an interesting link between the nerve damage and cutaneous abnormalities seen in this rare

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1994.tb08452.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryTrichoepitheliomas (TE) are benign follicular neoplasms which are frequently confused with basal cell carcinomas (BCC). It is important to distinguish these entities precisely, as the treatments and prognoses are different. To this end, we stained a series of formalin‐fixed, paraffin‐embedded specimens of unequivocal TE and BCC with antibodies directed againstbcl‐2, an oncogene associated with programmed cell death, and known to be overexpressed in some malignant tumours. The TE showed staining of tumour cells limited to the outermost layer of the proliferation. The BCC tumour cells demonstrated diffuse staining throughout the tumour nodules. This difference in staining pattern was then applied to more equivocal cases, and seemed clearly to separate the entities. The observed findings may prove to be of diagnostic help in distinguishing borderline cases, and also offer some possible explanations for the biological differences between these neop

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1994.tb08453.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1994.tb16141.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SUMMARYWe present epidemiological data for 5197 families with psoriasis. Errors in reporting have been analysed. Analysis of the data provides indications of random mating with respect to whether the partner has the skin disease or not. Data on psoriasis among parents, siblings and children are provided, and particular attention has been paid to the age at onset of psoriasis. Psoriasis was present in the parents of about 36% of the probands. In families in which one or both parents have psoriasis, the occurrence of the disease among the siblings does not provide any information which differentiates between a dominant and recessive mode of inheritance, but is compatible with both. In families in which neither parent had psoriasis, provided there was a proband with psoriasis, the probability of the siblings suffering from psoriasis was close to 0.25, indicating a recessive mode of inheritance. The distribution of psoriasis among the parents of all probands, and among the children of probands, was also compatible with this mode of inheritance. The prevalence of psoriasis in the elderly was estimated to be about 5%, and the gene frequency in the whole population 25%.These findings show that, with regard to first‐degree relatives, the inheritance of psoriasis can fit an autosomal recessive model. The concept of a recessive mode of inheritance may be used as a basis for genetic counsellin

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1994.tb08454.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryWe have examined the action of cantharidin on the skin of patients with Darier's disease, and used immunohistological techniques to determine the distribution of desmosomal components, keratin intermediate filaments, and proteases in cantharidin‐induced blisters. Cantharidin induced acantho‐ lysis, but the presence of acantholysis did not trigger the development of the characteristic warty, dyskeratotic papules in patients with Darier's disease.The distribution of desmosomal components. keratins and proteases within the acantholytic keratinocytes in the cantharidin‐induced blisters was similar to that previously found in acantholytic cells within lesions of Darier's disease: peripheral staining for extracellular desmosomal components was reduced: some desmosomal components were detected diffusely in the acantholytic cells: basal cell keratin markers were expressed by some suprabasal acantholytic cells, and plasminogen was detected in association with acantholytic cells. Cleavage of desmosomes did not reveal the underlying abnormality in Darier's di

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1994.tb08455.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1994.tb16142.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY