摘要:

SUMMARYUsing a new method devised by our laboratory, the ultrastructure of dermal glycosaminoglycan in an involved area of lichen myxoedematosus was examined.Although histochemical and biochemical studies have indicated simply an accumulated deposition of hyaluronic acid in the lesion, the glycosaminoglycan ultrastructure within it was distinaly different from that in normal skin. The glycosaminoglycan structure of normal skin was similar to the proteoglycan aggregate model described by Rosenberg (1975). As confirmed by the enzymatic digestion procedure, it represents the ultrastructure of hyaluronic acid bound to glycosaminoglycans such as dermatan sulphate or chondroitin sulphate. In contrast, hyaluronic acid filaments observed in lesions of lichen myxoedematosus contained no glycosaminoglycan subunits.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01281.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYEpidermal thymidine incorporation, as a measure of DNA synthesis, and ornithine decarboxylase activity were estimated in hairless albino mice following phototoxic reactions induced by topical anthracene+UV‐A, and topical 8‐methoxypsoralen (8‐M0P)+UV‐A, Both treatments caused depression of epidermal thymidine incorporation to 26% of control values at 4 h; this depression persisted through 24 h following 8‐MOP+UV‐A. Animals treated with anthracene + UV‐;A showed a fourfold increase in thymidine incorporation at 48 h, declining at 72 and 96 h; after 8‐MOP+UV‐A increased thymidine incorporation was observed between 4 and 10 days, when a plateau of 96 h duration was observed. After treatment with anthracene‐f‐UV‐A, epidermal ornithine decarboxylase activity (ODC) was maximal at 4 h, and exhibited a rapid decline, with normal levels at 48 h. Following 8‐MOP, UV‐A dose‐dependent ODC induction occurred: this was later than that induced by anthracene + UV‐A with no detectable activity at 4 or 12 h, and maximum activity at 24 h, the elevation persisting through 96 h. The relationship between ODC induction and epidermal hyperproliferation followin

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01282.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYWe have previously reported the effect of hydrocortisone (HC) on the adenylate cyclase system of pig epidermis. HC had no effect on the basal level of cyclic AMP but there was an increased adrenalineinduced cyclic AMP accumulation when epidermal slices were incubated for more than 6 h with HC. This incubation did not alter the responsiveness to histamine. We are interested in the effects on epidermal cells of HC, which may act through the adenylate cyclase system. It is well documented that adrenaline, histamine and adenosine stimulate adenylate cyciase and cause an accumulation of cyclic AMP in pig epidermis. An increased cyclic AMP results in the inhibition of mitosis and epidermal outgrowth. Employing the rate of epidermal outgrowth and mitotic index as indicators of cyclic AMP effect, we studied the effect of HC on the adenylate cyclase system. The pretrcatment with HC (100 μM) magnified the inhibitory effect of adrenaline on epidermal outgrowth and mitosis, whereas the inhibitory effeet of histamine or adenosine was not affected by HC. It is suggested that HC induced an increased responsiveness of adenylate cyclase to adrenaline, resulting in a magnified inhibitory effect of adrenaline on epidermal outgrowth and mitosis

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01283.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYTreatment with an oral aromatie retinoid (etretinate) and with PUVA significantly reduced the elevated levels of putrescine, spermidine and spermlne in psoriatic lesions. Both treatments also significantly reduced the spermidine/spermine ratio, which is considered to be an indicator of proliferation activity. Although both regimens produced a roughly parallel reduction of epidermal polyamines the initial fall of putrescine was much more rapid in patients receiving retinoid. This may indicate that one of the primary targets of retinoids could be ornithine deearboxylase.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01284.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYA technique is described for the selective killing of fibroblasts in primary cultures and subcultures of human keratinocytes using a monoclonal antibody raised against human fibroblasts. The antibody kills fibroblasts by complement mediated cytotoxicity but is not toxic to keratinocytes.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01285.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYAn effect of colchicine on the active Arthus reaction in rabbits was investigated in vivo with simultaneous evaluation ofin vitrochemotacdc activity of polymorphonuclear leukocytes. Arthus reactions were induced by intracutancous injection of ovalbumin into rabbits prcimmunized with ovalbumin. Colchicine, 1 mg/kg or 2 mg/kg, injected intraperitoneally one hour prior to the induction of the Arthus reaction, suppressed erythema and induration at 12 h, when histology showed decreased numbers of infiltrated polymorphonuclear leukocytes and no apparent vascular damage. Perivascular deposits of C3, suggestive of the presence of immune complexes, persisted to 24 h.In vitrochcmotaxis of polymorphonuclear leukocytes using Boyden chamber techniques revealed suppression of the activity in the colchicinc–treatcd animals at 6 and 12 h.These findings suggest that colchicine inhibits vascular injury by interfering with directional chemotaxis of polymorphonuclear leukocytes to the lesional site

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01286.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYThe effects of treatment with topical 2,4–dinitrochlorobenzene (DNCB) were observed in fifty–one patients with alopecia areata of at least 9 months duration. Patients were sensitized either by the application of 500 μg of DNCB in acetone to the forearm, or by painting affected areas of the left side of the scalp with a 1 % solution. A mild to moderate dermatitis was maintained by weekly applications of DNCB.When the sensitizing dose was applied directly to the scalp, significantly more patients showed poor reactivity as judged by the eczematous response obtained, although the two routes of sensitization had comparable effects upon regrowth of hair.Hair regrew significantly more frequently in females. The likelihood of regrowth was reduced in patients with hair loss of long duration and in those with immunological disturbances such as autoantibodies or low T lymphocyte numbers and responses. The relationship of these factors to the disease and to the response to DNCB treatment is discu

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01287.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYA simple method was developed for the determination of steroid sulphatase activity in a skin biopsy for routine use. In this method, 6 mg of minced tissue is incubated in i ml of Krebs‐Ringer bicarbonate buffer with radioactive dehydroepiandrosterone sulphate (20,000 c.p.m., S.A. 1.1 Ci/mmol) for 4 h. After the incubation the liberated unconjugated dehydroepiandrosterone and its possible metabolites are separated from the conjugated compound by extraction with ethyl acetate‐ethyl ether 10:90 (v/v). The organic phase is counted in a scintillation counter. The results are expressed as c.p.m. values per 6 mg tissue wet weight.Steroid sulphatase activity was measured in skin biopsies from nine control subjects and from thirteen patients with various types of ichthyosis. In the different groups studied, the ranges of the c.p.m. values were as follows: controls (n = 9) 377–1802; X‐linked ichthyosis (n = 5) 140–214; ichthyosis vulgaris (n = 5) 607–1320; ichthyosiform erythroderma (n = 2) 2146–2214; lamellar ichthyosis (n = i) 2185. The reagent blank varied from 180 to 259 c.p.m. In X‐linked ichthyosis the c.p.m. values were always less than in the corresponding reagent blank, indicating that no enzjrme activity was present in the tissue. In other types of ichthyosis, steroid sulphatase activity was normal. The method described is easy to accomplish in any clinical laboratory where scintillation count

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01288.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYTwo patients with urticaria evoked at the site of contact of skin with water have been studied. Protection of the skin from contact with water by prior application of petrolatum ointment prevented wealing, but removal of the stratum corneum enhanced wealing.Organic solvents did not themselves evoke wealing, but they enhanced the reaction to subsequent challenge by water.That the release of acetylcholine is an essential step in the pharmacogenesis of wealing in aquagenic urticaria is indicated by the suppressive effect of locally‐applied scopolamine on water–evoked wealing.Aquagenic urticaria is also associated with elevated blood histamine levels and degranulation of mast cells in the water–challenged skin.The relationship of acetylcholine and histamine to each other and to contact of water with the skin remains unce

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01289.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYIn this study we have shown that in pili annulati all the normal layers within each cuticle cell are present, and show a normal distribution of cystine. The presence of well ordered macrofibrils, composed of microfibrils embedded in a cystine rich matrix, in both regions of the hair shaft indicates that normal keratinization can and does take place. We have demonstrated the presence of electronopaque, cystine positive material at the intermacrofibrillar spaces and suggest that the presence of the cortical holes is in part due to insufficient cortical material to occupy the space available, and in part due to the dissolution of the intermacrofibrillar material, possibly by water or other chemical agents, diffusing through an apparently more permeable cuticle.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01290.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY