摘要:

SUMMARYOur clinical and immunological studies of 114 cases of systemic sclerosis, 54 of Raynaud's disease and 46 of other connective tissue diseases, centered on the diagnostic and prognostic significance of anticentromere antibodies (ACA). The ACA occurred in 21 of 84 patients with acrosclerosis, in four of 54 patients with Raynaud's disease but in none of 30 patients with diffuse scleroderma or transitional form, acrosclerosis‐diffuse scleroderma, or 46 cases of other connective tissue diseases. The ACA‐positive patients had no contracture or immobilization of the fingers, the indurations and/or indurative oedema were confined to fingers and usually no other types of ANA were detected. However, systemic involvement and the course of the disease were comparable in ACA‐negative and ACA‐positive acrosclerosis patients.The studies indicate that there is a subset of acrosclerosis with minimal indurations confined to the fingers, and ACA appears to be its serological marker. We propose to use the term CREST for this subset, which to date has not been exactly defined and is regarded by some authors as synonymous with acrosc

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1985.tb02351.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYThe purpose of this study was to investigate whether 8‐methoxypsoralen (8‐MOP) plus long‐wave ultraviolet (UV‐A) light irradiation (PUVA) had a direct effect on the expression of epidermal cell and basement membrane zone (BMZ) markers without altering intracellular metabolic pathways. Cryostat sections of guinea‐pig skin were covered with an 8‐MOP solution and irradiatedin vitrowith UV‐A light. Treated sections were stained subsequently with various membrane markers including pemphigus sera, pemphigoid sera (BMZ marker) and a group of biotin‐labellcd lectins (Concanavalin‐A, peanut agglutinin, soybean agglutinin,Ulex europeusagglutinin (UEA) and wheat germ agglutinin). Fluorescent staining with all reagents except for UEA was reduced appreciably in intensity by PUVA radiation at higher energy doses (20 μ/ml of 8‐MOP plus 20–30 J/cm2of UV‐A). Radiation with lower energy doses did not affect membrane marker staining. Weakened or abolished marker staining was restored to normal when scavengers of oxygen intermediates such as superoxide dismutase (540–5400 units/ml) or sodium azide (10–200 mM) were added to the 8‐MOP solution during UV‐A irradiation. UEA staining was negative in both PUVA‐ irradiated and untreated guinea‐pig skin. PUVA at higher energy doses appeared to cause tissue damage by photodynamic reactions, resulting in

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1985.tb02352.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYDermo‐epidermal separation in dystrophic epidermolysis bullosa (DEB) is thought to result from an abnormality of anchoring fibrils which, in normal skin, are stained selectively by the periodic acid‐thiosemicarbazide‐silver proteinate technique. This method was applied to non‐lesional skin from four patients with generalized recessive DEB, five with localized recessive DEB and one with dominant DEB. No anchoring fibrils were present in the subjects with generalized recessive DEB, but they were morphologically normal in the case of dominant DEB and in three patients with mild localized recessive DEB. Two patients with a more severe form of localized recessive DEB possessed attenuated anchoring fibrils which lacked the normal banding of the mid‐portion. The lamina densa, normally unlabelled, was consistently stained in patients with generalized recessive DEB, suggesting a biochemical abnormality of this

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1985.tb02353.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYThe concentrations of vitamin A and total carotenoids were measured in serum and skin of 61 patients with acne vulgaris, atopic dermatitis, ichthyosis vulgaris or lichen planus, and compared with those in 37 healthy subjects. The mean serum concentrations of retinol and retinol‐binding protein were significantly decreased in patients with acne (P<0.01) and slightly increased in those with ichthyosis (P<0.05), but were otherwise normal. Serum carotenoid levels did not differ between patients and controls. Superficial shave biopsies from both involved and uninvolved skin were examined for the presence of retinol (vitamin A1), dehydroretinol (vitamin A2) and total carotenoids. The mean retinol concentration was increased in lichen planus lesions (P<0.05) and decreased in both acne skin (involved and uninvolved) and in lesions of atopic dermatitis (P<0.05). The mean dehydroretinol concentration was markedly increased in lesions of atopic dermatitis and lichen planus (P<0.01). No consistent abnormalities were found in skin of patients with ichthyosis vulgaris. The mean carotenoid concentration in the patients' skin did not differ significantly from that in the controls. The reduced retinol level in the skin of acne patients is probably explained by diminished supply of vitamin A from the blood. The abnormal ratio of retinol to dehydroretinol in lesions of lichen planus and atopic dermatitis is possibly due to changes in cutaneous vitamin A metabolism associated with epidermal hyperproliferation and inflammatio

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1985.tb02354.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYWe studied the elicitation of contact sensitivity toCandida albicansantigen in guinea‐pigs with experimental cutaneous candidiasis and in humans, using commercially available potent 1: 100C. albicansantigen (Torii) by patch testing on abraded skin. In guinea‐pigs, non‐immune animals became patch test‐reactive 4–5 days after topical application of viableC. albicans, either under occlusion or without occlusive dressings, concurrently with the demonstrability of delayed responses to intradermally injected 1: 10 000Candidaantigen. In humans, all healthy adults who showed delayed hypersensitivity reactions to intradermally injected 1:10 000C albicansantigen demonstrated positive patch‐test reactions to 1:100C. albicansantigen. There was a significant correlation between the magnitude of responses to these tests. In contrast, no positive patch test reactions were elicited to the 1: 100C. albicansantigen on neonatal skin, emphasizing the lack of irritability of this test agent. These results also indicate that in humans contact sensitivity toCandidaantigen occurs during later life becauseC. albicansis a ubiquitous organism. The practical value of thisCandidapatch test for evaluation of patients' immune function was assessed by a prospective study in patients with various skin disorders. The results obtained suggested some potential value of the test for evaluation of cell‐mediated immune function of patients with regard to ubiquitous re

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1985.tb02355.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYIn toxic epidermal necrolysis (TEN), as in the ‘epidermal type’ of erythema multiforme, the necrotic epidermis is infiltrated with mononuclear cells. We studied the epidermal infiltrate in seven cases of TEN. About half the cells obtained from pieces of cleaved epidermis dissociated by trypsin were non‐epithelial. On cytologic analysis, 80% of these foreign cells exhibited markers of macrophages, 15% were granulocytes and only 5% were lymphocytes (almost exclusively OKT8 T lymphocytes). Semi‐thin sections of early prenecrotic lesions showed exocytosis of mononuclear cells within the epidermis with features of satellite cell necrosis and formation of colloid bodies. Almost all these mononuclear cells were macrophages as evidenced by endogenous peroxidase‐positive granules. These findings suggest that some kind of macrophage‐mediated cytotoxicity may play a role in the necrosis of epidermal cells

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1985.tb02356.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYIn a study of 46 patients with nodular prurigo (NP), potential metabolic causes of pruritus, such as anaemia, hepatic dysfunction, uraemia and myxoedema, were present in 50%. Focal causes of pruritus were important in 37% and included insect bites, venous stasis, folliculitis and nummular eczema. Psycho‐social disorders were recorded in over 50% of patients and were considered relevant in 33%. Clinical and histological appearances ranged from classical NP to chronic lichenified eczema. Neural hyperplasia was not a prominent featur

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1985.tb02357.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYA semi‐quantitative, immunoperoxidase monoclonal antibody technique was used to study the mononuclear cells surrounding 32 basal cell carcinoma specimens from 30 patients. Tumours were analysed in subgroups based on recurrence, size and ulceration. T cell counts were high (>3 out of 4) for all groups while T helper/inducer and T suppressor/cytotoxic cell counts were equal (approx. 2). Macrophage counts were low for all groups, about 1.2, while B cell and Ia positive cell counts were high (>3). T/B cell and T helper/suppressor cell ratios approached one for the tumours as a whole as well as the sub‐groups. The relative importance and contribution of cell mediated vs. humoral immunity in keeping basal cell carcinomas in check is discus

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1985.tb02358.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYTwenty‐five patients with immunologically classified B cell chronic lymphocytic leukaemia (CLL) were examined for skin lesions. Six showed evidence of cutaneous involvement, and histological examination of skin biopsies form these patients revealed perivascular lymphocytic infiltration with some diffuse involvement in three. Immunohistological analysis with a range of B and T cell specific monoclonal antibodies revealed that in all cases the infiltrate was predominantly T cell in origin and that epidermotropism in three cases was also associated with T cell components. Six control patients with macroscopically normal skin were also biopsied and these biopsies subjected to the same assessment. All were norma

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1985.tb02359.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYMalignant histiocytosis is a rare, and usually fatal, tumour which may involve the skin. The clinical and pathological changes are described in five patients who presented with skin nodules as the initial feature. We witnessed spontaneous healing of lesions, a previously described phenomenon which may cause diagnostic confusion. It is suggested that patients presenting with malignant histiocytosis confined to the skin form a sub‐group with a more favourable prognosi

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1985.tb02360.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY