ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb03194.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SUMMARYImmunohistological staining of frozen sections of normal human skin demonstrated the prescnce of significant numbers of mononuclear cells cxpressing novel epitopes associated with CD4‐positive suppressor‐inducer functions. The cells were located around superficial vessels and within the basal layers of the epidermis and hair follicles. The antigen identified by the various antibodies has been shown to be functionally important in the induction of various suppressor cells capable of abrogating Bcellresponses to pokeweed mitogen. The presence in the skin of cells with possible down‐regulatory functions in the immune response may be significant with respect to surveillance against neoplasms and control of appropriate responses to infectious a

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb03195.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SUMMARYRecombinant human IFN‐γ, used for treatment of melanoma and renal carcinoma, was found to induce HLA‐DR expression on human keratinocytesin vivo.HLA‐DR antigens bound to keratinocytes of the basal and suprabasal layers of the epidermis were observed after intramuscular or intravenous injections of 0.5 mg/kg body weight IFN‐y, 3 times a week. Keratinocyte‐bound HLA‐DR antigens were ftrst observed at the beginning of the third or fourth week of treatment, but HLA‐DQ and HLA‐DP antigens were never detected on keratinocytes. The intracytoplasmic constant (lO chain of the class II molecules was also not detectable within the keratinocytes. Paticnts who received IFN‐α2therapy, did not exhibit keratinocyte‐

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb03196.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SUMMARYMucosal involvement in linear IgA disease was assessed clinically and immunologically using direct and indirect immunofluorescence (IF) techniques. There was clinical evidence of oral mucosal involvement in all 10 patients examined and conjunctival disease in six. Direct IF findings correlated well with clinical oral disease, with all patients demonstrating linear IgA deposits in the basement membrane zone of oral mucosa, However, this was not true of conjunctiva where no linear IgA could be demonstrated. Conjunctiva did provide a good substrate for indirect immunofluorescence using patients' sera, and showed that five of the ro patients had circulating anti‐basement membrane zone Ig

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb03197.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SUMMARYPhospholipid‐sensitive, Ca++‐dependent protein kinase activity was investigated in the cytosol of melanoma cells, A protein kinase system was partially purified, and enzyme activity was found to be modulated by palmitoyl‐carnitine.In order to link the actions of palmitoyl‐carnitine on phospholipid‐sensitive protein kinase activity and the already reported role of protein kinase C in cell division, we studied the action of palmitoyl‐carnitine on melanoma cell growth by measuring colony forming ability in a soft agar culture system. Palmitoyl‐carnitine was found to inhibit cell growth in a dose‐dependent manner.These findings suggest that palmitoyl‐carnitine (or long‐chain acylcarnitine), a naturally occurring metabolite, may play a key role in the onset of cell division.We suggest that the action of palmitoyl‐carnitine on phospholipid‐dependent protein kinase activity is in part related to the molecular events linking protein kinase C activity and the ionic events in the in

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb03198.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SUMMARYSixteen patients with chronic idiopathic urticaria were skin tested with their own serum, 10 with autologous plasma and five with serum that had been heated to 56° C to inactivate complement. Eight showed a weal and flare response to whole serum, four to plasma and five to heat‐treated serum. All serum‐positive patients showed the same response to their own plasma and to heated serum, indicating that the mediator concerned is not generated by clotting and is not dependent on a functioning complement pathway. Three control subjects were negative to autologous serum, plasma and heated serum. Local tachyphylaxis was demonstrated in five serum‐positive patients on reinjection of the same site with autologous serum on 3 consecutive days. This raises the possibility that the serological mediator may be acting by mast cell degranulation or directly on receptors in blood vessels and that repeated injections could induce a change in the number of receptors. Passage of whole autologous serum from four serum‐positive patients through ultrafiltration membranes showed that fractions with a molecular weight of less than 30,000 daltons were still able to produce a positive skin test response, but those less than 1000 daltons were not. All serum fractions from two serum‐negative patients and three normal controls were negative. Whole autologous serum from five serum‐positive patients and two control subjects was separated by column chromatography. On skin testing with pooled fractions, the greatest response was produced by fractions of 10,000–15,000 daltons in the serum‐positive patients, but there was no response

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb03199.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SUMMARYWe carried out ophthalmological examinations, and histopathological examinations of skin biopsies in 32 male patients affected by X‐linked ichthyosis.We found corneal opacities in only five patients, and their presence was not related to the age of the patients. Skin histology revealed a reduction of the granular layer in nine cases, a finding previously thought to be typical of autosomal dominant ichthyosis vulgari

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb03200.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SUMMARYForty‐nine patients with atopic dermatitis entered a double blind lacebo controlled cross‐over study of mupirocin, a new topical antistaphylococcal antibiotic. Forty‐five patients were evaluable. Quantitative bacteriological assessment before treatment showed that heavy colonization of the skin with Staphylococcus aureus was present in nearly all patients even in the absence of overt infection. However, the bacterial count was significantly reduced by 2 weeks' treatment with topical mupirocin, but not by the placebo. Moreover, a significant reduction of clinical severity was also observed after treatment with mupirocin, which was maintained over the following 4 weeks, although recolonization occurred during this period, with bacterial counts rising to pre‐treatment levels. Despite recolonization, clinical deterioration was not observed during the trial period. No serious side‐effects were observed.Phage typing showed that 50% of patients carried more than one bacterial phage type. Recolonization in eight patients (17%) was with a ‘new’ strain that had not previously

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb03201.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SUMMARYWe investigated the in vivo effect of recombinant interferon‐γ (IFN‐γ) and tumour necrosis factor α (TNF‐α) treatment of mice on the development of the delayed‐type hypersensitivity (DTH) reaction and lichenoid tissue reaction (LTR) following the local injection of cloned autoreactive T cells. Both the DTH reaction and the LTR were significantly enhanced by pre‐treatment with IFN‐γ, but not with TNF‐ã. Induction of class II MHC antigens on keratinocytes was not essential for the enhancement by IFN‐γ. Administration of anti‐IFN‐γ antibody reduced the DTH reaction and LTR, although complete inhibition was not observed with our treatment regimen. The ability of IFN‐γ to increase the number of the cloned T cells invading the epidermisin vivo, is in keeping with our previous observation that IFN‐γ treatment of cultured keratinocytes markedly increased the adherence reaction between

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb03202.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SUMMARYThe clinical, histological and immunological effects of long‐term treatment with thymopentin (TP‐5), administered 50 mg i.v. three times a week on alternate days, in four patients with Sezary syndrome is reported.In all four cases reduction of itching, oedema, scaling and thickening, and clearing of erythroderma were noted after 2 months treatment. Peripheral blood Sezary cells decreased in three cases. Reduction or suspension of the drug was followed by a clinical relapse.A loss of epidermotropism and a reduction in cell infiltrates were observed together with a dramatic reduction in epidermal and dermal Langerhans cells.An increase in the proliferative response to mitogens and in IFN‐y production, and the expression of activation antigens in PHA stimulated cultures occurred after 3 months. HNK‐ 1 + cells increased both in the peripheral blood and in the dermis following a transient increase in IL‐2 receptors, suggesting that clinical response in TP‐5 treated patients may be mediated by an incrased production of IL‐2 and consequent generation of cytotoxic cells or release of lymphokines able to augmen

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb03203.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY