摘要:

SUMMARYWe have investigated the effects of PUVA therapy on human Langerhans cell (LC) immunophenotype and function. Epidermal sheets were obtained from exposed, and control shielded, forearm skin at the end of a course of PUVA therapy, in patients receiving treatment routinely for a variety of dermatoses. PUVA therapy decreased the overall number of HLA‐DR+CD1a+LCs in epidermal sheets, and in epidermal cell (EC) suspensions examined using a fluorescence activated cell sorter (FACS). PUVA therapy also reduced the overall EC allostimulatory capacity in the allogeneic epidermal cell‐lymphocyte reaction (ELR), and the capacity of ECs to present tetanus toxoid to, and augment concanavalin A‐mediated stimulation of, lymphocytes in the autologous ELR. Depressed allostimulation by ECs from PUVA‐treated skin could not be restored by indomethacin (added to block prostaglandin synthesis). The reductions in LC numbers and EC allostimulatory capacity varied according to dose, and time since cessation, of PUVA therapy, and in individual patients were of comparable degree. By contrast, the allostimulatory capacity of residual LCs from PUVA‐treated skin (purified using the FACS) did not differ from that of purified control LCs. PUVA‐induced suppression of cutaneous immune responses, therefore, results at least in part from an overall impairment of EC antigen‐presenting capacity. Residual HLA‐DR+CDla+LCs in PUVA‐treated skin which retain their alloantigen‐presenting function may represent a subgroup of PUVA‐resistant LCs; alternatively, these cells may be as yet unaffected because they have only recently migrat

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb04157.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThis study examined the effects of low‐protein diet on the population density, morphology and histochemical characteristics of Langerhans cells. Weaned at 18 days old, BALB/c mice were divided into two groups: one group received a high‐protein diet (20% casein) and the other was fed an isocaloric low‐protein diet (caesin 4%). After 14 days, the mice were killed and the skin of the ears was removed for investigation. Langerhans cells were visualized using ADPase and anti‐la immunoperoxidase techniques. In protein malnourished mice, the density of ADPase and la‐positive Langerhans cells was significantly reduced, while morphometric assessment of their cross‐sectional area showed a significant reduction of total cell area, cell body area and degree of arborization. Quantitative cytophotometric analysis revealed a reduction of ADPase ectoenzyme activity and decreased concentration of membrane Ia antigen. We postulate that these changes affect Langerhans cell functions, and in turn influence the immune mechanisms

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb04158.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYIn an attempt to identify more clearly the origin of cells in human basal cell carcinoma xenografts in mice, paraffin and frozen sections were subjected toin situDNA hybridization with biotin labelled human and murine DNA probes. Human skin and mouse skin sections were used as controls. As expected, the implanted epithelium reacted with the human DNA probe and the surface epithelium and most of the stromal cells reacted with the murine probe. However, the stroma immediatety surrounding the implanted epithelium contained cells of human origin mixed with murine cells. Occasional murine cells (presumed inflammatory) were present in the human implanted epithelium. Assessment showed no correlation between the degree of differentiation of the implanted epithelium and the ratio of human/murine cells in the contiguous stroma. This technique provides a sensitive test for identifying human cells in xenografts and may be useful in assessing the role of stromal cells in the differentiation of a variety of carcinomas.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb04159.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYTwenty children (mean age 9 weeks) with infantile seborrhoeic dermatitis (ISD) were investigated with cultures for bacteria,Pityrosporum ovaleand other fungi, and 20 healthy children served as controls.P. ovale and Staphylococcus aureuswere the dominant organisms.P. ovalewas cultured in 18 of 20 infants with ISD as compared to 4 of 20 controls.S. aureuswas cultured in 14 of 20 infants with ISD as compared to 1 of 20 controls. The role ofS. aureusin ISD is not known, but it could be a secondary invader as is supposed in atopic dermatitis (AD). Even ifP. ovalemay be suspected as the aetiological agent of ISD further studies are needed to clarify the exact role of the organism in ISD.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb04160.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe final α‐keratin compositions attained in the outer horny layer of 16 dyskeratoses have been compared with the series of compositions which is produced during normal epidermal differentiation. In each case, the abnormal outer horny layer composition corresponded with that of normal basal, spinous, granular or inner horny cells, with, in some cases, the addition of α‐keratins characteristic of hyperproliferating/regenerating keratinocytes. The results have implications for the aetiology of these diseases. In addition, the distinction between the α‐keratin patterns in certain of the conditions was sufficiently clear to allow the use of the technique as a non‐invasive aid to

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb04161.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYWe have investigated the differences between the sexes in the development of contact sensitivity induced by dinitrochlorobenzene (DNCB). Ten male and 12 female subjects were sensitized with DNCB (30 μg applied on a 1 cm patch test disc) and challenged 1 month later with doses of 8.8, 12.5, 17.7 and 25 μg. The responses were measured after 48 h as increase in skinfold thickness with Harpenden callipers. Females showed a larger response at all challenge doses studied, and the slope of the log‐dose response curve was significantly steeper in females. We conclude that there are significant differences in delayed type hypersensitivity between males and fema

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb04162.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYFish handlers frequently suffer from hand warts. The clinical form and HPV type in these lesions were studied. Eleven individuals (10 fishmongers and one fisherman) with multiple hand warts were examined clinically and samples from their warts examined by Southern blot and reverse blot analysis. Clinically, with one exception, the warts were of the common type. HPV DNA was detected in all but one individual. HPV4 was found in one sample, HPV1 related virus in three, a virus hybridizing with both HPV27 and HPV2 in five (four individuals) and HPV7 in seven (six individuals). More than one type was detected in four individuals. HPV7 infection was related to the greater length of time spent in handling fish. These findings indicate that HPV7 is not, as was previously thought, found exclusively in those handling butcher meat and suggest that environmental conditions may be a factor in the clinical manifestation of HPV7 infection. The exact nature of a virus designated HPV2/27 and the significance of its presence in these fish handlers remains uncertain.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb04163.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYFifty‐two lesions of solar keratosis from 40 patients were studied prospectively for the presence of Darier‐like changes and acantholysis. Twenty‐seven per cent of specimens showed some degree of these changes. The affected lesions tended to be larger and were less common on the hands, but were otherwise clinically indistinguishable from other solar keratoses. Histotogically, the specimens with Darier‐like changes were significantly thicker and more dysplastic. The autoradiographic labelling index was not different for the lesions with these changes indicating similar cell kinetic characteristics.It is suggested that acantholytic separation of epidermal cells within a solar keratosis may signify that the lesion has a greater invasive potential than a solar keratosis not showing this

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb04164.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYDirect and indirect immunofluorescent studies (DIF, IIF) were performed on 24 pemphigus vulgaris patients who were in a state of clinical remission. The tests were repeated after an interval of 6 months. All the patients were on maintenance therapy with oral prednisone. The DIF in eight patients showed negative results among whom seven remained negative. Six patients out of 24 showed weakly positive fluorescence and ten patients showed strong positive fluorescence. The IIF was negative in 17 patients and positive in seven patients who also showed positive DIF. During a follow‐up period of 20 months, one of eight patients with negative DIF relapsed compared with two of six patients with weak positive DIF and five of 10 patients with strong DIF. Five patients with strong DIF for IgG also had C3, of whom three relapsed, compared with five of 19 patients who were negative for C3. Four of seven patients with positive IIF relapsed compared with four of 17 with negative IIF. It is suggested that repeated DIF tests in pemphigus patients, who are in clinical remission, may serve as an indicator for the immunological activity and be of help in the management of these case

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb04165.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYDiphencyprone is a potent topical sensitizer, but is non‐mutagenic in the Ames test (unlike dinitroclorobenzene) and remains relatively stable in solution (unlike squaric acid dibutyl ester).Seventeen patients with total loss of scalp hair (eight alopecia totalis, nine alopecia universalis) were treated by maintaining on one side of the scalp an allergic contact dermatitis induced by 2,3 diphenylcyclopropenone‐I (‘diphencyprone’), and on the other side an irritant contact dermatitis using tretinoin gel (Retin A). After 20 weeks, treatment with tretinoin was stopped and diphencyprone was applied bilaterally for a further 10 weeks.Satisfactory regrowth of terminal hair on the scalp was achieved in only one patient. Eyebrow, eyelash and beard regrowth was achieved in one individual whilst in another, moderate, but not cosmetically satisfactory, scalp regrowth took place. In no patient did regrowth take place at tretinoin treated sites until after diphencyprone was subs

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb04166.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY