ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb01768.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

summaryUsing a method which allowed us to study the morphological consequences of the expression and the inhibition of proteases in living tissues, we demonstrated that the primary detectable cellular event in cantharide acantholysis is the dissolution of the dense plaque, leading to the detachment of tonofilaments from desmosomes. This process is inhibited by neutral serine protease inhibitors.This suggests that the desmosome‐tonofilament complex, more precisely the desmosomal dense plaque, is the primary target of activated proteases during cantharide acantholysis, and can be disrupted by a specific epidermal protease‐anti protease system. Cantharide acantholysis may be a useful model for studying desmosomal turno

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb01769.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

summaryUsing a hypomitotic agent, triamcinolone acetonide, and a hypermitotic agent, retinyl propionate, we investigated the relationship between epidermal mitotic activity and stratum corneum renewal time of topically treated skin as determined by the dansyl chloride staining technique.Treatment with the base cream resulted in a reduction in renewal time compared with an untreated control site. The predicted increase in renewal time with the hypomitotic agent and reduction with the hypermitotic agent was only observed when daily treatment was commenced 2 weeks prior to and continued after dansyl chloride staining and not when treatment was started after staining.These results indicate that in order to use cell renewal methods to demonstrate changes in mitotic activity brought about by topical treatments, it is necessary to pre‐treat the skin with the test material to establish full epidermal equilibrium at the changed mitotic state before labelling with dansyl chloride. Meaningful claims for effects on cell renewal of specific cosmetic ingredients should only be made after comparison with a base cream treated site, both having been allowed to equilibrate, rather than on the basis of comparison with untreated ski

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb01770.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

summaryThe tissue distribution and species expression of the antigens in adult linear IgA disease (LAD) and chronic bullous dermatosis of childhood (CBDC) was studied and compared with those of bullous pemphigoid (BP) and epidermolysis bullosa acquisita (EBA) antigens.Positive sera from five adult LAD patients, 15 CBDC patients, three BP patients and two EBA patients were studied, using skin and mucosal tissues of humans and animals as substrates.The tissue distribution and phylogenetic distribution of adult LAD antigen and CBDC antigen were the same, suggesting that they are identical diseases. The antigens were both demonstrable only in stratified squamous epithelia and amnion of mammalian species. They were absent from transitional and columnar epithelium.The EBA antigen was not expressed in pig tissues or human placenta, unlike adult LAD and CBDC antigens. Expression of adult LAD, CBDC and BP antigens was observed in all tissues except bladder, which lacked LAD and CBDC antigens.These results suggest that the antigens in adult LAD and CBDC are the same and differ from BP and EBA antigens.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb01771.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

summaryTwenty patients with severe plaque psoriasis were selected to receive either low‐dose cyclosporin A (CyA) or placebo (CyA vehicle) in a double‐blind randomized trial at two centres.Within 4 weeks the mean reduction in the Psoriasis Area and Severity Index (PASI) in 10 patients receiving CyA (mean dose 5.5 mg/kg/day) differed significantly from the mean reduction in 10 patients receiving placebo. In eight patients given placebo a switch to CyA therapy resulted within 4 weeks in a mean reduction in PASI of 90%. In a total 15 out of 18 patients given CyA (83%) (mean dose 5.6 mg/kg/day) there was an improvement of ≥ 75% in PASI within 4 weeks. In a 2‐month tapering off phase a lower mean CyA dose (3 mg/kg/day) was effective in maintaining the reduced PASI scores in seven of nine patients. Four out of five CyA treated patients who entered a post‐treatment observation phase had a relapse (PASI score ≥ 50% of score at baseline) after a mean interval of 6.5 weeks.The most important side‐effects were mild reversible hypertension in 5 of 18 patients (28%), and reversible elevated serum creatinine levels in 7 of 18 patients (39%). We consider that further studies are justified in severe chronic psoriasis to establish suitable regimens for maintenance of remission in psoriasis with low‐doses of CyA or a combination of CyA with other anti

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb01772.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

summaryThe relationship between body weight and blood pressure, total serum cholesterol, triglycerides, uric acid and glucose was investigated in 203 psoriatic patients in comparison with 904 healthy controls. In the psoriatic patients, these parameters were clearly related to their body weight. Those psoriatic patients of normal weight exhibited lower mean values of all the parameters compared with overweight psoriatic patients while they did not differ from control subjects of normal weight. Our results suggest that only overweight psoriatic patients exhibit the metabolic abnormalities frequently reported in the literature, while psoriatic patients of normal weight do not differ from the general population in this respect.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb01773.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

summaryOne hundred and four consecutive patients with psoriasis who were being treated in two Dublin dermatology hospital units answered a questionnaire. Social and emotional morbidity was present for many patients despite access to modern treatment facilities. Fifty five percent of patients had never experienced a complete remission from their psoriasis. A large percentage of patients avoided common social activities, for example swimming and sports; 50% felt that psoriasis had inhibited their sexual relationships and 11% of patients said they would avoid having children in case their offspring should develop the condition. Thirty‐six per cent felt the hospital doctor spent most of the consultation time writing prescriptions and 59% of patients had never been given an explanatory pamphlet or book about psoriasis.To help patients to come to terms with what is for many a lifelong condition, greater efforts should be made to improve the communication at consultation, and provide more patient educatio

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb01774.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

summarySeventy one papules in the early stages of inflammatory development were isolated from acne vulgaris affected skin and their content of micro‐organisms characterized. The progress of lesions prior to excision was monitored by tracing an area of the upper back onto a transparent acetate sheet. This template was used the next day, and in some cases after 3 days, to identify inflamed lesions of less than 1 day and 2–3 days duration. These were biopsied, and pilosebaceous units isolated by micro‐dissection, homogenized and microbial populations studied by viable counting and microscopy.Propionibacteria colonised 68% of‘1 day’duration lesions and 79% of‘3 day’duration lesions; staphylococci colonized 19% and 32% respectively andPityrosporumspp.(Malassezia furfur)were found in 52% and 68%. Although the prevalence of each microbial group was higher in the more chronic lesions, these differences were not statistically significant. The microbial profile of inflamed lesions was similar both qualitatively and quantitatively to non‐inflamed lesions studied previously. These results call into question the role of micro‐organisms as the initiators of inflammation

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb01775.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

summaryTwenty nine patients with cicatrizing conjunctivitis were studied; 17 with a clinical diagnosis of cicatricial pemphigoid, five with a clinical diagnosis of pseudopemphigoid caused by long‐term application of topical medication and seven who had a cicatrizing conjunctivitis from other causes. Biopsies from clinically uninvolved bulbar conjunctiva were taken for direct immunofluorescence and blood was taken for indirect immunofluorescence using normal human conjunctiva, oral mucosa and skin as substrates.On direct immunofluorescence,in vivobound immunoglobulins were found along the basement membrane in 10 of the 17 patients with cicatricial pemphigoid, one of the five with pseudopemphigoid and two of the seven with a cicatrizing conjunctivitis associated with other diseases. Circulating anti‐basement membrane zone antibodies were found only when conjunctiva was used as a substrate. These were present in seven of the patients with cicatricial pemphigoid, three of those with pseudopemphigoid and two of those with a cicatrizing conjunctivitis caused by other diseases.These results indicate that direct immunofluorescence is a useful, but not absolute diagnostic marker for ocular cicatricial pemphigoid. The results in the pseudopemphigoid group argue that this is an immunologically mediated disorder indistinguishable from spontaneous cicatricial pemphigoid and probably triggered by the drugs. The presence of circulating antibodies should allow for precise identification of the antigen involved in cicatricial pemphigoid using SDS electrophoresis and Western blot analy

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb01776.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

summaryIntestinal calcium absorption increased significantly following 14 days of treatment with photochemotherapy (PUVA) in seven patients with chronic psoriasis. Irradiation with UVA alone over a similar period did not result in significant changes in calcium absorption in an additional seven prosiatic patients. These findings suggest that the increase in serum 25‐OH vitamin D levels previously reported in patients treated by PUVA may have important biological consequence

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1988.tb01777.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY