摘要:

SUMMARYThe presence of amyloid P component (AP) in dermal deposits of cutaneous atnyloidosis was demonstrated by a direct immunofluorescence technique using an antibody to serum amyloid P component (SAP). AP was also shown, for the first time, to be a constituent of normal human skin. It was present at the periphery of dermal elastic tissue fibres, in basement membranes of dermal blood vessels and surrounding eccrine sweat glands but was absent from the dermo‐epidermal basement membrane. The staining pattern in cutaneous amyloidosis was morphologically distinctive and readily distinguishable from staining of thickened vascular basement membranes in porphyria. Itnmunofluorescence with anti‐SAP is simple and specific and may become the procedure of choice in the differential diagnosis of amyloido

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01195.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYA cell line of a squamous cell carcinoma of human skin (HSC‐1) has been establishedin vitroand successfully maintained prolifcrativc in continuous tissue culture for ahout 21/2years since March 1978. Another cell line (HSC‐1b) has been establishedin vitrofrom the recurrence of the same cancer. It has been maintained for about 8 months since December, 1979. 3T3 feeders were used in the initial cultures in both cases. The cells grow in a monolayerin vitroand are epithelioid with anaplastic features. Chromosome analysis of HSC‐i, frozen and thawed HSC‐1, the transplanted tumour in a nude mouse and HSC‐1b all showed hypotetraploidy with the modal number 80, which reconfirms that HSC‐1 derives from the original skin tumour.The cell line is available to other in

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01196.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYSpontaneous genome mutations in ten patients with porphyria cutanea tarda (PCT) amounted to 1 02% as compared to 0.36% in the control group of healthy individuals (P<0.01). Spontaneous structural chromosome aberrations in patients were seen in 2.39% of the examined cells versus 0.70% in the cells of controls (P<0.01). The increased percentage of cells with spontaneous genome and chromosome mutations in patients with PCT cannot be associated with alcohol only.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01197.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYBiochemical investigations on the lipid and dioxin content of the cysts and comedo‐like lesions of three children exposed to tetrachloro‐2,3,7,8‐p‐dibenzodioxin (TCDD) have been performed.Gas chromatography and mass spectrometry failed to demonstrate the presence of dioxin despite the high sensitivity of the method. Further investigation by thin layer and gas chromatography‐mass spectrometry showed that the lipids of the chloracne lesions in these children were exclusively epidermal in origin.These findings support the hypothesis that exposure to dioxin produces a hyperproliferative reaction of the cutaneous epithelium with squamous metaplasia of the cells lining the ducts of sk

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01198.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYT lymphocyte numbers and functions were measured in forty‐six patients with alopecia areata and thirty healthy controls. In patients with alopecia areata, lymphocyte reactivity to extracts of scalp and hair follicles was not detected by3H thymidine incorporation.3H Thymidine incorporation by lymphocytes cultured with PPD, Varidasc and C.albicanswas significantly reduced in cells from patients compared with controls and correlated with the extent of hair loss and the presence of antithyroid antibodies. Decreased lymphocyte responses to PPD and Varidase were not due to the presence of suppressor monocytes because removal of monocytes with carbonyl iron did not increase lymphocyte reactivity. However, responsiveness to Calbicanswas augmented by removal of monocytes.T cell numbers were significantly lower than in controls (P<0.005); patients with auto‐antibodies had fewer T cells than patients without them.The relationship of reduced T cell function and auto‐immunity to alopecia areata is disc

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01199.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYThe prevalence of auto‐antibodies against thyroid constituents, gastric parietal cells, smooth muscle cells, mitochondria, reticulin, nuclear constituents and rheumatoid factor in 108 patients with alopecia areata was compared with that found in a previous survey of the local population. Female patients had a significantly increased prevalence of anti‐thyroid antibodies which were present in 30% overall and in 44% of the youngest age group (11–17 years). Smooth muscle antibodies were more frequent in female patients but the increase was not significant. Male patients had a significantly increased prevalence of thyroid and gastric parietal cell antibodies (11.4% each). In females, antithyroid antibodies were associated with extensive hair loss: they were found in 42% of female patients with total alopecia and only 20% of males with total hair loss.A family history of alopecia areata was obtained from 24% of patients; 10% had relatives with thyroid disease and 10% had diabetic relatives. These findings confirm the association between alopecia areata and the other auto‐immune d

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01200.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYTo determine the significance of circulating immune complexes in dermatitis herpetiformis, serum samples from thirty patients with active disease were tested by a CIq binding radioassay, while serum samples from twenty‐one of these patients were tested by a monoclonal rheumatoid factor (mRF) inhibition radioassay. By direct immunofluorescence, all patients demonstrated typical IgA deposition in dermal papillae. Using the Ci q binding assay, only seven of forty‐two serum samples had elevated Ci q binding activity, while by the mRF inhibition assay, thirteen of twenty‐five samples had elevated immune complex levels. Nine of these latter thirteen positive serum samples, however, were minimally elevated. Thus, IgG and/or IgM containing immune complexes are infrequently present, or at very low levels, in sera of patients with active dermatitis herpetif

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01201.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYSkin lesions were produced by application of 50% potassium iodide to twelve patients with dermatitis herpetiformis (DH). Perivascular cellular infiltrates were found to be characteristic of developing lesions. The cells were mainly round cells; α‐naphthyl acetate esterase staining revealed that in 24‐h lesions the mean percentage of T‐lymphocytes was 43%, that of mononuclear phagocytes 6% and that of non‐T/non‐M cells (mainly B‐lymphocytes), 44%. The percentage of the latter was highest (mean 81%) in 6‐h specimens, suggesting that these cells are participating in the early stages of lesion formation. The infiltrating cells in dermal papillae and within subepidermal vesicles were predominantly polymorphonuclear leukocytes (mean 86%), with some mononuclear phagocytes and non‐T/non‐M cells.Immunofiuorescence examination confirmed that fibrin deposition is characteristic of the initial lesions of DH and showed that the same is true of fibronectin. Seven out of eight patients had fibronectin deposits in dermal papillae. IgA was found in all and Ci in most of the specimens and, with the exception of papillary vesicles and blister cavities, tbe intensity of IgA and C3 fluorescence showed no marked alterations during the deve

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01202.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYPhagocytosed immune compiexes in polymorphonuclear leukocytes of nineteen patients with Raynaud's phenomenon were detected by scoring the cells for IgG and C3 inclusions. Parallel estimations of circulating immune complexes were made by liquid phase CIq binding and polyethylene glycol (PEG) precipitation methods. The results were compared with the degree of cold stress‐induced vasospasm measured by quantified infra‐red thermography. Thirteen of nineteen patients with Raynaud's phenomenon compared with only one of seventeen normal controls showed significantly elevated levels of phagocytosed IgG. The correlation of IgG and C3 scores for individual patients was high and the IgG and C3 scores correlated well with the thermographic assessment of the severity of the Raynaud's phenomenon. Two of the patients had elevated levels of circulating immune complexes detectable by raised levels of CIq binding, four patients had raised IgG and three patients raised IgM compiexes detectable by PEG precipitation. None of the controls had elevated levels of circulating immune complexes detectable by either method. There was no correlation between the levels of circulating and phagocytosed immune comple

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01203.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYLundh tests of pancreatic exocrine function were performed on twenty unselected patients with systemic sclerosis. Three patiems had very low levels of tryptic activity in their ititestinal juice and only nine had results which were unequivocally normal.Eight patients had biochemical steatorrhoea, but in six this was associated with intestinal bacterial overgrowth and a seventh had primary biliary cirrhosis. The remaining patient had no cause for steatorrhoea other than the marked pancreatic insufficiency which had been demonstrated.Although pancreatic damage may contribute to malabsorption in systemic sclerosis, it appears to be less important than other factors such as intestinal bacterial overgrowth.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb01204.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY