摘要:

SUMMARYThe optimum experimental conditions have been investigated for the measurement of the birth‐rate in human psoriatic epidermis using the vinca alkaloids vinblastine and vincristine. The dose response characteristics of the two drugs were assessed over a 2.5 h collection period; although the dose response characteristics differed between the two agents, a dose of 1μg injected intradermally was considered to be optimal for both. The linearity of metaphase correction was assessed for both agents, taking biopsies for up to 10 h after injection. It was found that there was a delay period for up to an hour which must be avoided during the analysis to prevent an underestimate of the birth rate. However, linearity was then demonstrated for up to 4 h after injection. Metaphase degeneration was assessed by isolating a cohort of unlabelled cells in the G2phase and tracing their fate over the next 10 h. It was found that the minimum life of an arrested metaphase was of the order of 4 h, and then metaphases degenerated at the rate of approximately 0.4% per hour. It is recommended that metaphase arrest experiments in psoriatic epidermis be confined to 4 h although, if required, correction factors are available to correct for experiments which are lengthened. Finally it is shown that flux into DNA synthesis exceeds the flux into mitosis by four or five times; several explanations are considered, and it is thought that the most likely explanation involves the death of psoriatic cells in the G2pha

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb00943.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYA range of doses of vincristine (0.5–10 μg) or colcemid (5–100 μg) injected intradermally into normal volunteers failed to yield any significant accumulation of arrested mitoses. This was despite an apparently successful block of cells in metaphase by the highest three doses of each drug. Possible reasons for the failure to see accumulation are discussed. The results suggest that normal human epidermis is not a suitable system for application of the metaphase‐arrest technique using intradermal in

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb00944.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYEpidermal proliferative kinetics were studied in a patient with pityriasis rubra pilaris. Elevated proliferative indices (flash labelling and mitotic indices) were apparent compared with normal epidermis and measurements of the birth rate indicate that the rate of epidermal cell production was similar to that found in psoriasis, and equalled 11.8 cells/1000 germinative cells/h; this suggests a cell cycle time of 60 h.Contemporaneous measurements of the rate of entry into DNA synthesis showed values similar to the rate of entry into mitosis; hence there is no G2cell loss as appears to occur in the hyperproliferative psoriatic epidermis.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb00945.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYSerum levels of retinol‐binding protein (the specific carrier of vitamin A) were measured in eleven patients with pityriasis rubra pilaris and in some of their close relatives. The level of retinol‐binding protein was markedly reduced in every patient, and in some of the relatives. It is postulated that defective synthesis of retinol‐binding protein is a biochemical marker for pityriasis rubra pilaris, probably transmitted as a Mendelian dom

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb00946.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYMorphometric analysis of lymphoid cells in the skin was used to differentiate between cutaneous T cell lymphomas (CTCL), i.e. mycosis fungoides (MF) and Sézary syndrome (SS), and chronic benign skin diseases. In electronmicrographs of the skin lesions from twenty patients with CTCL (group I), fourteen patients with chronic benign skin diseases (group II) and twenty‐nine patients suspected of CTCL (group III), the degree of nuclear indentation of lymphoid cells, expressed as the nuclear contour index (NCI), was measured.Analysis of the NCI histograms of the infiltrating cells of group I and group II permitted us to derive classification criteria for allocating all patients correctly with a high probability (≥95%) in the appropriate group. Only one case was classified with a low probability (71%). The classification criteria are based on the presence of cerebriform mononuclear cells (CMC) with highly indented nuclei (NCI≥11·5) and the frequency distribution of CMC in the skin infiltrates expressed as the 25th and the 70th percentiles of the NCI histograms (P25 + P70).When these criteria were tested on twenty‐nine patients suspected of CTCL, twenty cases were classified as malignant, nine as benign. During the follow‐up period, out of the twenty patients classified as malignant, seventeen patients appeared to have or develop MF, whereas two patients had lymphomatoid papulosis and one patient is still suspected of MF. Of the nine patients classified as benign, eight patients were proven to have benign skin diseases whereas one developed MF in the follow‐up period of up to 4 years.The classification results based on morphometry proved to be more sensitive than those based on DNA cytophotometry. It is concluded that morphometric analysis of lymphoid cells in the skin is of diagnostic relevance in the differential diag

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb00947.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYFlow cytometry was used to measure the DNA content of epidermal keratinocytes from psoriatic patients. Gross deviations were found in the lesions and minor but significant changes in the uninvolved skin. Statistical analysis revealed that the rather large variation in the DNA distributions of the lesions was due to inter‐individual differences rather than to inter‐individual differences. The duration of the S‐phase seemed to be prolonged in the lesion, but the length of the overall cell cycle might be of the same order as that of normal

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb00948.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYUpper limb buds of mouse embryos (day 11 + 3 h of development) were cultured for 6 days. During this time the epidermis develops from a two‐layered stage, consisting of a basal cell layer and a periderm cell layer, to a multilayered squamous epithelium with a stratum granulosum and a stratum corneum. To investigate the cell kinetics of epidermis and periderm during epidermogenesis the limb buds were labelled with3H‐thymidine at different stages of development. The migration of labelled cells was studied on day 3in vitro. In the first period of development, before a stratum granulosum has differentiated, each individual cell layer of the epidermis has a cell cycle of its own, i.e. once it has developed each cell layer grows independent of the other. The switching from horizontal to vertical proliferation starts on day 4 of culture with the appearance of the stratum granulosum and is completed on day 5 when a corneal layer begins to develop. With the appearance of the stratum corneum the limb bud shows the typical proliferation of the adult epidermis, which is regenerating only from the basal layer.The labelling behaviour of periderm cells also shows that these cells have a cell cycle of their own and are not formed by cells migrating from the epidermis in an upward direct

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb00949.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYA type of reaction of the skin to superficial trauma is described which differs from ordinary dermographism. This is more easily evoked by rubbing and consists of an erythematous band in which diffuse wealing develops which can be palpated and more easily demonstrated by stretching the skin. Ordinary dermographism is excluded since a scratch as usually employed to demonstrate this produces erythema and not a well‐defined raised weal. Forty patients who showed this reaction are described and it was also seen in three out of 100 normal subjects teste

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb00950.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYMinimal erythema dose (MED), immediate pigment darkening (IPD) and skin typing were performed on 130 patients with malignant melanoma and on seventy‐three controls. The tumours were histologically classified according to Clark as superficial spreading melanoma, nodular melanoma, and lentigo maligna melanoma. The tumours of twelve patients were not possible to classify. Patients belonging to skin type IV and patients with lentigo maligna melanoma had a significantly higher mean age. The IPD reaction was significantly higher in skin type II than in skin types III and IV both in patients and in controls. IPD and MED showed no significant correlation to age and sex. MED was significantly lower among patients with malignant melanoma than controls. These data indicate that light sensitivity is of aetiological importance in malignant melanom

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb00951.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYThe optical performance of some commercially‐available tinted lenses is discussed. There is a wide variation in the UV absorbing power of sunglasses although adequate protection against solar UV‐A can be achieved with some, but not all, polarizing material. The darkness of a tint is not an indication of its likely performance within the ultraviolet bands and it is possible that lenses could be produced that lacked any significant tint yet absorbed strongly throughout the UV reg

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1981.tb00952.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY