ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00647.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryCollagen gene expression was studied in dermal fibroblasts derived from the inflammatory and sclerotic skin lesions of patients with localized or generalized morphoea. The levels of mRNA for type I collagen in early‐passage fibroblasts derived from inflammatory lesions were higher than those obtained from the uninvolved skin, whereas those fibroblasts obtained from sclerotic lesions were unaltered. No alteration in type I collagen mRNA levels was observed in late‐passage fibroblasts derived from the inflammatory lesions. The relative rate of collagen to total protein synthesis in earlypassage fibroblasts derived from inflammatory lesions was higher than that of fibroblasts from uninvolved skin, while no alteration or a slight decrease was observed in fibroblasts from the sclerotic lesions. The data suggest that the inflammatory reactions induce increased collagen synthesis by fibroblasts in the skin in sclerode

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00648.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe presence of intraepidermal collagen type VII has recently been used to define a subgroup of patients with mild dystrophic epidermolysis bullosa (DEB). This subgroup demonstrates virtual resolution of blistering during infancy despite often severe neonatal blistering. Using the antibody LH7.2, we have detected intraepidermal collagen type VII in five cases with DEB. These represent a much wider spectrum of clinical features and of intraepidermal and basement membrane zone (BMZ) staining patterns. Only one of our cases had features consistent with reported cases. Sequential skin biopsies from this case showed a marked change towards normal within 6 months of birth, paralleling the clinical improvement. The other four cases had sparse epidermal deposits of collagen type VII and included an affected foetus with autosomal recessive DEB. These findings suggest that the frequency of intraepidermal LH7.2 in DEB may be much higher than previously thought. The presence of abundant intraepidermal collagen type VII is of prognostic significance and can disappear over months. We recommend that biopsies of infants suspected of having EB are taken during the neonatal period.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00649.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryCalmodulin levels in cultured skin fibroblasts from patients with progressive systemic sclerosis (PSS) and healthy controls were measured by their ability to activate cyclic AMP‐phosphodiesterase. Calmodulin levels were significantly increased in PSS fibroblasts compared with normal control fibroblasts. The changes in calmodulin content of PSS fibroblasts were also assessed by a radioimmunoassay. These findings suggest that an elevated of calmodulin may play a role in the pathogenesis of PS

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00650.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummarySpecialized immunological assays are required for the accurate diagnosis of bullous dermatoses such as bullous pemphigoid (BP), epidermolysis bullous acquisita and bullous lupus erythematosus. The aim of this study was to analyse and compare the sensitivity of indirect immunofluorescence (IF) on salt‐split skin and immunoblotting for the detection of circulating autoantibodies in BP. Of the BP patients selected for the study, 74/79 (94%) had circulating autoantibodies detected by at least one of the two methods. Both methods had comparable sensitivity and detected BP‐specific autoantibodies in 82–85% of the patients. Because 20% of the patients were found to be positive by only one of the methods, both methods should be used in the diagnosis of BP. Indirect IF on salt‐split skin is easier to perform and is preferable in routine analysis, but Western blotting may be used as a complementary assay with sera showing no reactivity on salt‐s

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00651.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThirty patients with psoriatic arthropathy (PA) were age‐and sex‐matched with patients with plaque psoriasis alone to investigate a possible link between Sjögren's syndrome (SS) and PA. The results of clinical examination, Schirmer's test, maximal stimulated salivary flow rate, haematological and serological investigations were compared in the two groups. Median maximal stimulated salivary flow rate was significantly reduced in patients with PA suggesting that salivary glands are affected in this condition. One patient with PA ha

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00652.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummarySweating and vasoconstrictive responses to intradermal injections of adrenaline were studied in the normal‐looking back skin of young adult males with atopic dermatitis (AD) and in non‐atopic controls using five concentrations of adrenaline, and further in a subgroup of subjects with dry‐looking AD skin using two concentrations of adrenaline. The sweating responses were measured with an evaporimeter and the area of blanching determined with planimetry. The maximal sweating response in the AD group was elicited with the suprathreshold concentration of 5 × 10−6mol/l and in the non‐atopic group with a 10‐fold higher concentration. At the three higher concentrations the sweat rates levelled out in both groups and remained at a lower level in AD subjects. The sweating responses in dry‐looking AD skin were lower than in non‐atopics (P<0.001) and were also lower than in those with AD normal‐looking skin (P<0.05) with both concentrations.In contrast to sweating, the vasoconstrictive reactions showed similar dose‐dependent increases with all tested concentrations in AD patients with normal‐looking skin and in the non‐atopic controls. In patients with dry AD skin the vasoconstrictive responses were also similar to thos

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00653.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe effect of iron was studied in rats in a ROS‐initiated model of acute skin inflammation. Iron dextran was administered i.v. 24 h before the induction of the inflammatory response by intradermal injection of glucose oxidase attached to polyethylene glycol (GOD–PEG). Iron exacerbated the response at 24 and 48 h (P>0.001). Histologically, a similar picture was seen to that without iron except for an increase in tissue oedema and matrix destruction including the skin glands. Associated with iron loading was an increase in Perls stainable iron in the skin (P>0.025) and liver (P>0.001). However, skin inflammation without iron loading also increased skin iron levels (P>0.025). Total serum iron was decreased in iron‐loaded and GOD–PEG animals (P>0.01) and the unbound iron binding capacity (UIBC) increased (

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00654.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryA 56‐year‐old man presented with a 30‐year history of a slowly enlarging lesion on the sole of his right foot. A biopsy showed an anastomosing network of small cuboidal cells with the formation of occasional sweat ductal lumina and a marked fibrovascular stroma. The histological findings were interpreted as consistent with the diagnosis of an eccrine syringofibroadenoma. Using immunohistochemistry all the tumour cells were positively stained by the pan‐cytokeratin antibody Lu‐5 and an antibody to the cytokeratins 1/5/10/11. In addition the luminal ductal cells expressed cytokeratin 19 and CEA. Tumour cells were negative for cytokeratins 1, 7, 8, 13 and 18 and did not express vimentin and GCDFP‐15. The results indicate that the eccrine syringofibroadenoma is differentiated towards the dermal e

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00655.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe effect of prostaglandin inhibition, using topical indomethacin, on anthralin inflammation was studied. Indomethacin gel and gel base were applied to opposite flexor forearm skin sites of 11 volunteers for 2 h and then washed off. Anthralin and UVB were then applied to the gel‐treated skin and the anthralin‐ and UVB‐induced erythema and oedema were measured at 2, 6, 24 and 48 h using Harpenden callipers and a reflectance erythrometer. Prostaglandin inhibition was demonstrated by a significant reduction of UVB erythema at the indomethacin‐treated sites compared to the gel‐base‐treated sites. There was a small but significant reduction in anthralin erythema, but not oedema, at the indomethacin‐treated sites compared to the gel‐base sites. This study demonstrates that prostaglandins, and other inflammatory mediators because the inhibitory effect was small, are involved in anthral

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00656.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY