摘要:

SUMMARYHuman skin was irradiatedin vivowith a single UVB dose (100 mJ/cm2or 200 mJ/cm2) to examine simultaneously the antigen‐presenting function and interleukin‐1 (IL‐1) production capacity of irradiated epidermal cells (EC). Suction blisters were produced on irradiated areas on days 0, 3 and 7 after UVB. Irradiated EC were harvested and co‐cultured with autologous T lymphocytes in the presence of antigens (PPD, HSV) or mitogen (ConA). Culture supernatants were tested for IL‐1 activity using the thymocyte comitogenity assay. We found that a single 200 mJ/cm2dose of UVB caused an immediate suppression of the antigen‐presenting function of EC, but no alteration in their IL‐1 production capacity or surface marker expression (ATPase, CD1). PPD‐and HSV‐induced lymphocyte proliferation was decreased 70‐80% and ConA‐driven proliferation 30% when compared to non‐irradiated EC. However, this suppression was restored on days 3 and 7 after UVB irradiation, this being coexistent with an increased capacity of EC to produce IL‐1. It remains to be elucidated whether the immediate UVB‐induced photoimmunosuppression observed in the present study is due to inhibitory mediators or impaired memb

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01342.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYDNA histograms of skin and blood specimens from 64 patients with known or suspected cutaneous T‐cell lymphoma (CTCL) have been examined and compared with normal blood mononuclear cells and skin biopsy samples from 50 patients with various benign cutaneous conditions (i.e. patch test infiltrates, eczema, psoriasis, lichen planus, atopic dermatitis) in an attempt to establish whether DNA measurements by flow cytorntry may improve the early recognition of CTCL. The results indicate that right‐skewed G0/G1 peaks are seen frequently in both benign disorders and known and suspected CTCL. Such peaks may reflect increased stainability of DNA due to chromatin dispersion during cell activation and/or cell proliferation and do not constitute reliable evidence of malignancy. In contrast, discrete aneuploid DNA peaks are confined to malignant lesions, but are seen almost exclusively in the advanced stages in which the diagnosis can be establignohed easily based on routine histological criteria. These data indicate that DNA measurements by flow cytometry is of only limited help in the early recognition of CTCL and support the view that the lymphoid infiltrate in early CTCL may be reactive (rather than neoplastic) or alternatively may contain only minor populations of abnormal (malignant) cells which cannot be detected by currently available DNA measurement techniq

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01343.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYSignificantly elevated activity of peripheral blood mononuclear cell cAMP specific phosphodiesterase (PDE) activity has been previously reported in adults with atopic dermatitis and in the cord blood of children born to atopic parents. We have been unable to confirm such a significant elevation in children with atopic dermatitis, and we have not found any correlation with dermatitis severity, age or total serum IgE.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01344.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYKeratinocyte expression of the monocyte/macrophage surface antigens defined by OKM1 and OKM5 antibodies (Ortho Diagnostics) was examined using the peroxidase anti‐peroxidase immunohistochemical technique. A range of inflammatory cutaneous disorders were investigated, including lichen planus, psoriasis and atopic dermatitis. Positive suprabasal keratinocyte expression of OKM5 antigen was observed in all disorders, while keratinocyte staining with OKM1 antibody was consistently negative. These results provide further evidence that keratinocytes may play an important role in cutaneous immune responses. Furthermore, they are consistent with the recent observation that HLA‐DR positive keratinocytes may modulate cutaneous immunological reactions by inducing T‐cell unresponsiv

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01345.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYEight patients with intensely pruritic lesions of chronic idiopathic prurigo nodularis and three patients with neurodermatitis circumscripta were investigated using the indirect immunofluoresence method. Results showed similarities in epidermal hyperplasis but not in nerve proliferation and neuropeptide immunoreactivity. Increased numbers of calcitonin generelated peptide (CGRP) and substance P immunoreactive nerve fibre bundles were detected in specimens taken from prurigo nodularis lesions, but no increased immunoreactivity could be seen in specimens taken from patients having neurodermatitis circumscripta compared to normal skin. The neuropeptides, CGRP and substance P, may be responsible for the intense itching of prurigo nodularis lesions.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01346.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYRoutinely used formal saline fixation reduces the number of demonstrable mast cells in human skin by up to 30% compared with paried specimens fixed in Carnoys medium. Using metachromatic (toluidene blue), orthochromatic (alcian blue/safranin), enzymatic (chloroacetate esterase reaction) and immunofluorescence (berberine and fluorescein conjugated avidin) staining techniques, mast cells were demonstrated and quantified. Alcian blue/safranin and fluorescein‐conjugated avidin were both superior to the other staining methods used. We recommend the use of Carnoys medium fixed tissue stained with either alcian blue/safranin or conjugated avidin for optimal visualization and assessment of mast cells in human ski

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01347.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYA condition of multiple benign and sometimes self‐healing infundibular tumours of the skin is described. It affects the sun‐exposed areas of the head and neck in Caucasians. The individual lesions consist of a cluster of thick pockets, representing in many details the supra‐sebaceous portions of the follicular unit. Follicular poroma, tumours of the deeper part of the follicular infundibulum, virus warts, inverted follicular keratosis and prurigo nodules do not correspond adequately with the appearances described in the present series of six

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01348.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYIsotretinoin differs from acitretin and R0137410 by its striking sebostatic effect in acne after oral, but not topical, administration. The reason for this is not yet understood.Previous studies indicate that cellular retinoic acid binding protein (CRABP) might be implicated in the action of synthetic retinoids. We, therefore, compared the three retinoids for their ability to increase epidermal CRABP levels after systemic and topical treatment. Oral treatment with acitretin and R0137410 led to a striking increase of epidermal CRABP (from 2.6±0.9 to 16.2±2.9,P<0.004 and from 2.5±1.2 to 21.5±3.4 pmol/mg protein,P<0.004, respectively), while isotretinoin failed to induce a comparable rise (3.2±1.6 before and 3.7±0.7 pmol/mg protein after treatment), although it displayed in all patients a striking sebostatic effect. After topical application, the increase of CRABP was comparable for all three compounds. The interaction of isotretinoin with the epidermis seems to be different from other synthetic retinoids only after systemic treatment, a finding that parallels clinical observa

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01349.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYPatients with localized morphoea have an increased incidence of auto‐immune disorders, but this is not related to the age of onset, duration of disease nor the site or number of lesions. The patients have an increased incidence of tissue antibodies and these are more common when more than one lesion is present. The relatives of the patients have also been found to have an increased incidence of auto‐immune disorders.These findings suggest an immunological basis for the disease and indicate that the patients should be investigated and followed up to detect the evidence of other auto‐immune diso

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01350.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYPlasma dehydroepiandrosterone sulphate, androstenedione, testosterone (T), dihydrotestosterone (DHT), and sex hormone binding globulin (SHBG) have been measured in 64 females and 26 males aged less than 25 years and with acne vulgaris. Oestradiol was measured in the males. Free T and free DHT were calculated. Acne was graded on three sites and the sebum excretion rate (SER) was measured in most patients. With the possible exception of free DHT, none of the plasma steroids or SHBG correlated with acne severity or with SER. Free DHT in the females showed a possible, but weak, correlation with total acne (r=0.25,P=0.07), but comparison with male data showed that this was not causative. The role of androgens in acne is permissive and plasma androgen measurements usually have no place in its management.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01351.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY