摘要:

SUMMARYA method is described which permits the direct measurement of the activity of individual cytoplasmic enzymesin vivo.Those demonstrated include the cytoplasmic enzymes lactatc dehydrogenase, malate dehydrogenase, pyruvate kinase, glutamate oxalacetate transaminase and NADP‐dependent isocitrate dehydrogenase; whereas, the mitochondrial glutamate dehydrogenase and NAD‐dependent isocitrate dehydrogenase were not measurable. The reactions observed in our experiments seem to be mediated by an active transport of hydrogen across the cell membrane.In an extension of this technique to human epidermisin situ, we have shown an increased level of the intracellular LDH activity in psoriatic lesions, and demonstrated a marked reduction following the systemic administration of a single dose of prednisolone or methotrexate. Our method should find application in the following fields:1The study of regulatory mechanisms within the cell.2The elucidation of the cellular pathology in diseases such as psoriasis.3Investigation of the effects of pharmacological agents at a cellular le

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1974.tb12936.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

SUMMARYBiopsies of stratum corneum were taken from twenty‐four human volunteers, who had been patch tested for 3 h with two experimental detergent solutions coded A and B.The histochemical method for acid phosphatase demonstrated that both solutions damaged the stratum corneum, A more than B. It would seem that the staining reaction for acid phosphatase in the stratum corneum is a marker of the response of cells to damage by low irritancy compound

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1974.tb12937.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

SUMMARYThe human skin surface outside the areas bearing dermatoglyphics has been studied and an attempt made to explain the surface pattern in simple geometric and functional terms.The basic geometrical unit is a rectangle or square which in turn is subdivided by one or two diagonals. The triangles and diamonds thus formed undergo varying degrees of collapse under tension. These collapse units are sometimes composed of smaller polygonal compressible sub units with a distinctly honeycomb appearance. The hexagonal shape of the individual horny cell is an ideal smallest structural unit within this geometric system. The regional differences are considerable, but can be explained by the regional differences in the mechanical requirements. These features are of considerable functional significance for the stretching and deformation of the essentially inelastic stratum corneum

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1974.tb12938.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

SUMMARYA further instance of familial scleroderma is reported. Three sisters in a female sibship of six were involved. Possible reasons why scleroderma in families may be more common than previously supposed are discussed.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1974.tb12939.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

SUMMARYSeven patients with a bullous photodermatitis from nalidixic acid are described. Bullae develop characteristically on the dorsal aspects of the feet and on the lower legs, but also elsewhere, in patients taking the drug who are exposed to a high level of ambient sunlight. At times, the clinical picture may be confused with porphyria cutanea tarda. Abnormal reactions to sunlight may continue for several months after the acute stage, whether or not the drug is stopped, but some patients may continue to take the drug if direct sunlight on the skin is avoided.Investigations showed that long wave ultraviolet radiation appears to be responsible for the reaction and it proved possible to induce photosensitivity in three volunteers who took the drug for I week.The Honorary Editors ofThe Proceedings of the Royal Society of Medianskindly gave permission for details in the abridged version of this paper to be published.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1974.tb12940.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

SUMMARYPrevious studies from our laboratory suggested that architectural alterations of the cell membranes may have a major significance in the pathogenesis of psoriasis. Consequently, the membrane‐bound Mg++activated ATP‐hydrolytic activity was investigated in normal and psoriatic epidermis under the electron microscope.The present study revealed that, in contrast to normal epidermis, only minimal ATP‐hydrolytic activity is present on the cell membranes of psoriatic keratinocytes. This finding may reflect either a diminished attachment of substrate on the altered cell surface or an enzyme defect of the cell membrane. In both cases the reduced interaction between ATP and membrane‐bound ATP‐hydrolysing enzymes represents a functional membrane disorder of the psoriatic keratinocyte, presumably resulting in an insufficient utilization of ATP for active transport mechanisms on the cel

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1974.tb12941.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

SUMMARYRadio‐autographic techniques have been applied to the study of three hyperplastic human epidermal diseases; psoriasis, seborrhoeic keratoses and Bowen's disease. In each, a different alteration of nucleic acid and protein synthesis has been found when the abnormal tissue is compared to clinically normal contiguous skin. Psoriasis was characterized by stimulation of DNA, RNA and protein synthesis, while in mature seborrhoeic keratoses DNA synthesis was decreased at the same time as RNA and protein synthesis were increased. In Bowen's disease DNA and protein synthesis were enhanced, but the number of cells involved in RNA synthesis was decreased.Similar methods have been applied to psoriatic epidermis during treatment with vitamin A acid ointment. The medication resulted in inhibiton of DNA synthesis at a time when RNA and protein synthesis were enhance

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1974.tb12942.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

SUMMARYThe labelling indices of the epithelium of the lesions of oral lichen planus (LP) have been estimated, usingin vitrolabelling with tritiated thymidine. The relationship between this epithelial labeling index and the underlying inflammatory cell density was also examined. The total labelling activity in the LP lesion was comparable with that of normal mucosa but was redistributed as follows. The labelling index of the basal epithelial layer was increased above that of normal controls and the labeling in the suprabasal layer was decreased. Thus 50% of mitoses occur basally in involved atrophic LP epithelium. The implications of this redistribution of mitotic activity are discussed.The labelling index of epithelium was reduced where the inflammatory cell density was exceptionally high, but no simple inverse relationship between labelling index and inflammatory cell density was apparent. This supports the concept that the primary event in lichen planus seems to occur in the basal epithehal layer followed by an inflammatory cell response in the underlying connective tissue. Subsequent secondary damage to the epithelium, including inhibition of mitosis, may be produced by this inflammatory infiltrate only if it is very dense.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1974.tb12943.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

SUMMARYThe prevalence ofCorynebacterium acneson the foreheads of male and female children of caucasoid and negroid origin has been investigated using a semi‐quantitative technique. Few organisms were recovered from children of less than 12 years of age. The increase in count occurred earliest in Negroid girls and latest in caucasoid boys. This may be related to the age of pubert

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1974.tb12944.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

SUMMARYA case of purpura hyperglobulinaemica of Waldenström is reported. Immunofluorescence of skin and subcutaneous tissue using monospecific fluorescein‐tagged anti‐human IgA, IgG, IgM and complement showed deposition of IgA in the walls of intradermal vessel in affected skin. It is suggested that these findings help in the elucidation of the pathogenesis of the pur

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1974.tb12945.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY