摘要:

SUMMARYIn order to study the biological and possible pathological roles of cyclic adenosine 3′,5′‐monophsphate (cyclic AMP) in the skin, it is mandatory to measure cyclic AMP in 50–100 μg of microdissected epidermis, dermis or appendages.In the present study, we ofter a method of extracting cyclic AMP from less than 100 μg of tissue, removing contaminating nucleotides and scaling down Gilman's method to fit the analysis of small amounts of tissue. Cyclic AMP levels in the dermis, epidermis, and hair follicles (bulbs) were approximately 1, 2 and 3.5 pmols/μg dry weight tissue respectively.This procedure is applicable to the measurement of cyclic AMP levels in limited foci of healthy or dis

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb03072.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYThe cyclic adenosine 3′,5′‐monophosphate (cyclic AMP) content of pig skin was measured several seconds to minutes after removal of the skin from the body. It increased very rapidly, reached a maximum by 2 min after removal (4 times higher than the initial level), then decreased very slowly.Propranolol injected into the animal before or added after the removal of the skin did not suppress this phenomenon. The practical significance of this finding (increase of cyclic AMP level in skin after ischaemia) is obvious—in order to measure cyclic AMP levelin vivo, the sample must be frozen immediately to avoid an ‘artificial’ increase in

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb03073.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYSlices of human skin obtained with‐a keratome were pre‐incubated with [3H]adenine to label the ATP pool from which cyclic AMP was subsequently formed. The accumulation of radioactive cyclic AMP was measured as an index of adenyl cyclase activity. The data showed that both the ability to incorporate [3H] into ATP and adenyl cyclase activity were significantly lower in psoriatic plaques than in uninvolved skin of the psoriatic patients, or in normal skin of control subjects. The response of adenyl cyclase to the stimulation of 3.3 μM adrenaline was less than five fold in psoriatic plaques as compared to twelve to thirty‐two fold in the uninvolved skin. The response to the stimulation of prostaglandin E2 (5 μg/ml) showed no significant difference between the plaque and normal skin. The adenyl cyclase activity in uninvolved skin of psoriatic patients appeared normal. Propranolol (10 μM) blocked the stimulatory effect of adrenaline but not that of PGE2in normal skin. These resulsuggest that the adenyl cyclase system of the skin has different regulatory sites for adrenaline and PGE2, and that the enzyme is defective in the epidermis of the psoriatic plaque, especially at the adrenaline regulatory site. A preliminary report on this study was given at the Second International Conference on Cyclic AMP at Vancouver, Canada, 8‐11

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb03074.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYSkin lesions and clinically normal skin of thirteen patients with systemic lupus erythematosus (SLE) were examined, by the use of immunofluorescent techniques, to determine the presence of Ciq, C3, C3proactivator (C3PA), properdin, immunoglobulins (IgG, IgA, and IgM), and fibrin. IgM deposition was present in all thirteen skin lesions and in all eleven normal areas tested, whereas IgG deposition occurred in eleven of the lesions but in only six normal areas. IgA was the least frequently encountered immunoglobulin.C1qdeposition occurred in all thirteen skin lesions, and C3deposition was present in twelve. Seven of nine and eight of eleven clinically normal areas demonstrated C1qand C3deposition, respectively. Although less intense than C1qand C3deposition, properdin deposition occurred in eight of the thirteen skin lesions tested but in only two of nine normal areas. C3PA deposition was of greater intensity than was properdin, but was seen in only five lesions and three clinically normal areas. Seven skin lesions and two normal areas also demonstrated fibrin deposition. Although alternate pathway activation is. apparent, our findings suggest that the classical pathway (C1to C9) is the primary complement pathway involved in slli skin.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb03075.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYGrowth and survival of cutaneous neoplasms are dependent on the development of a nutrient‐supply ing vascular lifeline. A subcutaneous lymphoma is described which mimicked a vascular neoplasm because of the extensive telangiectasia overlying the tumour. Fragments of the lymphoma inserted in direct apposition to the hamster cheek pouch membrane or separated from the membrane by a microporous filter both induced dramatic neovascularization. Control materials failed to induce significant vascular changes. A diffusablc Tumour Angiogenic Factor may play a vital role in tumour survival and portends a therapeutic potentia

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb03076.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYOne hundred and twenty‐three children who had napkin dermatitis, with or without a secondary sensitization eruption, in infancy were reviewed 5–13 years later. Of the seventy‐one who had a predominantly psoriasiform secondary eruption, twelve (17%) had psoriasis at review—three (4%) had atopic eczema. None of the forty treated for a predominantly seborrhoeic secondary eruption had psoriasis at review—15 (37%) had atopic eczema. The psoriasiform group had the highest incidence of psoriasis and the lowest incidence of atopy among first degree relatives. The converse incidence was found in the seborrhoeic group.It is suggested that infants who develop psoriasiform napkin dermatitis have a psoriatic

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb03077.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYThis report reviews data relating to the nickel hazard from implanted prostheses. It is shown that nickel is released from stainless steel prostheses by the action of sweat, blood and physiological saline solution. In laboratory animals, solubilized nickel is localized in the tissue near the implant.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb03078.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYStudies on primary cultures of normal adult melanocyies from the ear skin of a black strain of guinea pigs are reported. Melanocytes have a low proliferation rate and tend to form cohesive groups on a glass surface. The cells undergo division while maintaining close contacts with each other whereas DNA synthesis is rare in melanocytes with multiple contacts.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb03079.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYStarting with large numbers of small split‐thickness explains of human skin, it is possible to grow ample numbers ol keratinocytesin vitroas primary cell cultures to permit biochemical studies without the need of subculturing technique

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb03080.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYThe changes that take place in skin explantsin vitroat the interface between migrating epidermal cells and dermis have been examined by light and electron microscopy and by immunofluorcscent antibody techniques. In the first 24 h epidermal cell migration occurred in the absence of any of the usual components of this junctional zone. After 48 h of incubation antigenic determinants of the basal zone were detected and basal lamina was demonstrated ultrastructurally. It is proposed that migrating epidermal cells do not need a complex and specialized junctional zone and that these cells are themselves responsible for the elaboration of new basal lamina.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb03081.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY