摘要:

SUMMARYBiopsies from twenty‐nine malignant skin tumours and four benign dermatoscs and fourteen samples of normal skin were stained for cell surface membrane or cytoplasmic expression of HLA heavy chain and light chain (β2microglobulin) by use of the immunoperoxidase technique on frozen sections using monoclonal antibodies.Results showed a toss of β2microglobulin from the surface of malignant cells in twenty‐four out of twenty‐nine cutaneous malignancies and the presence of HLA heavy chain in all twenty‐nine primary and secondary tumours. Expression of both heavy and light chains was observed in the four benign lesions and in normal epidermis in all samples examined. This would appear to be the first report of dissociation of heavy and light HLA chain expression in various forms of cutaneous m

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb04611.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYSkin calcium‐binding protein (SCaBP) is a low molecular weight protein of unknown function which is localized in the cytoplasm of epidermal basal cells. We have examined SCaBP immunoreactivity in basal cell carcinoma (BCC) since BCC cells retain some of the characteristics of basal cells, including the capacity for proliferation and migration from the dermo‐epidermal junction. We also examined immunoreactivity to basement membrane zone (BMZ) antigens (type IV collagen, laminin and bullous pemphigoid antigen) in BCC. We found that all BCC cells showed SCaBP immunoreactivity regardless of the histological type or position of the cells. BMZ antigens were expressed around tumourous buds, although bullous pemphigoid antigen may also present a cytoplasmic immunoreactivity. Our results suggest that SCaBP immunoreactivity is independent of the basal position of the cell, but may be related to the proliferative capacity and the undifferentiated state of the c

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb04612.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYEpithelial cells can be distinguished from various non‐epithelial cells by the presence of keratin‐type intermediate‐sized filaments, which can be detected by immunofluorescence microscopy, using antibodies to α‐keratin. In the present study, two types of antibodies were obtained. One of them was specific for α‐keratin (mol. wt. 49,000 to 69,000 daltons) in whole epidermis, and the other for α‐keratin (mol. wt. 62,000 and 69,000 daltons) in prickle and granular cells but not in basal cells. Four cases of so‐called mixed tumour of the skin were studied by immunofluorescence microscopy using these antibodies. Tumour cell nests of cuboidal and polygonal cells, tubular structures and keratinous cysts reacted with these antibodies, as did individually‐dispersed tumour cells within the myxoid and chondroid matrix. These results indicate that all the tumour cells of the so‐called mixed tumour of the skin are of epithelial origin. Differences in staining intensity between these tumour cells and their specificity for these two antibodies are discussed with reference to keratin differentia

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb04613.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYThe distribution of cytoplasmic microtubules in cultured guinea‐pig keratinocytes was investigated using immunofluorescence (IF) microscopy with monospecific anti‐tubulin antibodies and electron microscopy (EM). In culture, adherent cells displayed networks of thin fluorescent fibres, while a homogeneous and/or granular cytoplasmic IF was shown in the cells of upper layers as well as in trypsinized cells. By EM many microtubules were shown in adherent cells but there were fewer or none in the upper layers. An increase in calcium ion (Ca2+) concentration and the addition of an ionophore (X537A) to the culture medium caused disassembly of microtubules. This effect was cancelled by a calmodulin inhibitor. Cryostat sections of normal human and guinea‐pig epidermis stained with anti‐tubulin antibodies showed a homogeneous and/or granular cytoplasmic IF from basal to granular layers but no detectable IF was seen in the horny layer. These results suggest that keratinocytes contain a cellular pool of tubulin in various states of polymerization and that microtubule disassembly may occur during differentiation, probably being regulated by Ca2+‐calmodulin

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb04614.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYUsing a special selection technique, normal guinea‐pig melanocytes were maintained in highly purified but sparse cultures (˜104cells/25 cm2culture vessel) which showed little proliferative activity. The applicability of the Pomerantz tyrosinase assay was tested in this in vitro model system using three different approaches, namely crude cell extracts and viable cell cultures eitherin situor in suspension. The latter modifications both proved too insensitive, whereas crude cell extracts allowed accurate measurements of the basal tyrosinase activity and its stimulation by various agents. In unstimulated cultures basal tyrosinase activities ranged from 3O% to 7OO%(mean 260%) above the blank values; intra‐assay and inter‐assay variability were 4.2% and 77.5% respectively. Stimulation with α‐MSH (10‐5m, 10‐6m), β‐MSH (10‐5m), choleratoxin(10‐11M)and cAMP(10‐4M) plus theophylline(10‐4m) resulted in an increase of tyrosinase activity 30–65% above basal values. Melanotropin potentiating factor (10‐8m) enhanced the effects of α‐MSH (10‐6m) by 20%. This assay modification provides a sensitive tool for comparative studies of melanogenesis in normal melanocytes, malignant melanocytes

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb04615.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYSuction blister fluid was collected from normal human volunteers before (SBF) and 2 h after (SBF 8‐MOP) oral 8‐mcthoxypsoralen (0.6 mg/kg) ingestion without irradiation. In SBF 8‐MOP the concentration of 8‐MOP was 150 ng/ml, and fluorescent metabolites were also present. The toxicity of SBF 8‐MOP was then determined with and without UV‐A irradiation in the diploid strain D7of the yeastSaccharomyces cerevisiacwhich is suitable for the detection of lethal, mutagenic and recombinogenic events. It was compared with that of SBF, SBF with 8‐MOP addedin vitro(150 ng/ml) and 8‐MOP (150 ng/ml) in water. SBF showed no effect with UV‐A doses up to 360 kj m‐2; SBF 8‐MOP showed a slight decrease in survival and a dose‐dependent increase in nuclear mutations, mitotic gene conversion and crossing over; SBF with 8‐MOP addedin vitroshowed increased photobiological activity compared with SBF 8‐MOP. This photobiological activity was increased by a factor of six when using 8‐MOP in water. These results indicate a different bioavailability of 8‐MOP in water and in interstitial fluid containing proteins. The metabolites of 8‐MOP in human skin did not increase the photobiological activity of the drug. We conclude that the 8‐MOP present in human skin during PUVA therapy is mutagenic and

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb04616.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYThe unit area trichogram was defined in the frontal and occipital areas often normal men and ten normal women and the results compared with the findings in ten male and fifteen female patients with androgenic alopecia. The epilation technique provided accurate data of normal hair density (higher than previously reported) and the phases of hair growth. The measurement of hair diameter showed that of 7603 hairs, 1241 were less than 40 μ in diameter, of which only 21 were more than 80 mm in length. Variation in the diameter of individual hair fibres was recorded. The number of hairs less than 40 μ diameter per cm2of scalp was increased in both men and women with androgenic alopecia. It was concluded that the number of hairs greater than 40 μ in diameter per cm2(meaningful density) reflected most closely the amount of hair usefully contributing to an individual's clinical appearan

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb04617.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYSynthetic and natural retinoids were analysed in epidermis, dermis, subcutis and serum of twenty‐seven patients treated with etretinate (0·6–1·0 mg/kg/day) for 1–36 months. The concentrations of etretinate (including its major metabolite) in serum and normal‐appearing epidermis were 150–600 ng/ml and 50–350 ng/g, respectively. The serum and epidermal values were significantly correlated (P<0·05). The drug progressively accumulated in the subcutis attaining a maximum value of 15,500 ng/g. After the treatment, etretinate disappeared from the epidermis within i week. By contrast, the drug remained in the subcutis for several months after cessation of treatment. The epidermal composition of endogenous retinoids changed during etretinate therapy as reflected in an increased ratio of 3‐dehydroretinol to

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb04618.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYSix patients with keratoacanthoma were successfully treated with intralesional injection of bleomycin. This new therapy produced a marked shortening of the clinical course without any complications.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb04619.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYA 61‐year‐old woman is presented with essential cryoglobulinaemia and a 25‐year history of recurrent ulceration of the lower legs. She developed multiple benign angiomatous nodules on the face, earlobes and neck. Histologically, they simulated an angiosarcoma and are therefore of considerable diagnostic impor

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb04620.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY